LOCUS BBa_K1461229 2834 bp DNA linear 22-Nov-2024 DEFINITION resettable single counter system using ecf11 ACCESSION BBa_K1461229 VERSION BBa_K1461229.1 FEATURES Location/Qualifiers stem_loop 690..733 /label=stem_loop misc_feature 679..758 /label=BBa_B0010 terminator 679..758 /label=BBa_B0010 stem_loop 774..793 /label=T7 TE misc_feature 800..800 /label=stop polyA_site 794..807 /label=polya misc_feature 767..807 /label=BBa_B0012 terminator 767..807 /label=BBa_B0012 terminator 679..807 /label=BBa_B0015 promoter 1..35 /label=BBa_J23101 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..670 /label=BBa_K1461005 stem_loop 690..733 /label=stem_loop misc_feature 679..758 /label=BBa_B0010 terminator 679..758 /label=BBa_B0010 stem_loop 774..793 /label=T7 TE misc_feature 800..800 /label=stop polyA_site 794..807 /label=polya misc_feature 767..807 /label=BBa_B0012 terminator 767..807 /label=BBa_B0012 terminator 679..807 /label=BBa_B0015 misc_feature 44..807 /label=BBa_K1461202 misc_feature 1..807 /label=BBa_K1461212 promoter 816..895 /label=BBa_K1461002 misc_feature 908..911 /label=conserved RBS 904..915 /label=BBa_B0034 CDS 922..1663 /label=BBa_K1461008 stem_loop 1683..1726 /label=stem_loop misc_feature 1672..1751 /label=BBa_B0010 terminator 1672..1751 /label=BBa_B0010 stem_loop 1767..1786 /label=T7 TE misc_feature 1793..1793 /label=stop polyA_site 1787..1800 /label=polya misc_feature 1760..1800 /label=BBa_B0012 terminator 1760..1800 /label=BBa_B0012 terminator 1672..1800 /label=BBa_B0015 stem_loop 2717..2760 /label=stem_loop misc_feature 2706..2785 /label=BBa_B0010 terminator 2706..2785 /label=BBa_B0010 stem_loop 2801..2820 /label=T7 TE misc_feature 2827..2827 /label=stop polyA_site 2821..2834 /label=polya misc_feature 2794..2834 /label=BBa_B0012 terminator 2794..2834 /label=BBa_B0012 terminator 2706..2834 /label=BBa_B0015 CDS 1809..1896 /label=end of LacI coding region (inactive) misc_feature 1809..2008 /label=BBa_R0010 misc_feature 1897..1934 /label=CAP binding site promoter 1945..1950 /label=-35 promoter 1969..1974 /label=-10 misc_feature 1974..2008 /label=LacI binding site misc_feature 1981..1981 /label=start promoter 1809..2008 /label=BBa_R0010 misc_feature 2021..2024 /label=conserved RBS 2017..2028 /label=BBa_B0034 CDS 2035..2697 /label=BBa_K1461007 stem_loop 2717..2760 /label=stem_loop misc_feature 2706..2785 /label=BBa_B0010 terminator 2706..2785 /label=BBa_B0010 stem_loop 2801..2820 /label=T7 TE misc_feature 2827..2827 /label=stop polyA_site 2821..2834 /label=polya misc_feature 2794..2834 /label=BBa_B0012 terminator 2794..2834 /label=BBa_B0012 terminator 2706..2834 /label=BBa_B0015 misc_feature 2017..2834 /label=BBa_K1461207 misc_feature 1809..2834 /label=BBa_K1461224 ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgcgcatt accgcgagcc tgcgcacctt ttgccatctg agcaccccgc atagcgatag 121 caccaccagc cgcctgtgga ttgatgaagt gaccgcggtg gcgcgccagc gcgatcgcga 181 tagctttatg cgcatttatg atcattttgc gccgcgcctg ctgcgctatc tgaccggcct 241 gaacgtgccg gaaggccagg cggaagaact ggtgcaggaa gtgctgctga aactgtggca 301 taaagcggaa agctttgatc cgagcaaagc gagcctgggc acctggctgt ttcgcattgc 361 gcgcaacctg tatattgata gcgtgcgcaa agatcgcggc tgggtgcagg tgcagaacag 421 cctggaacag ctggaacgcc tggaagcgcc ggtggatcgc accctggatt atagccagcg 481 ccaggaacag cagctgaaca gcgcgattca gaacctgccg accgatcagg cgaaagtgct 541 gcgcatgagc tattttgaag cgctgagcca tcgcgaaatt agcgaacgcc tggatatgcc 601 gctgggcacc gtgaaaagct gcctgcgcct ggcgtttcag aaactgcgca gccgcattga 661 agaaagctaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 721 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 781 cttcgggtgg gcctttctgc gtttatatac tagaggcctc cacaccgctc gtcacatcct 841 gtgatccact cttcatcccg ctacgtaaca cctctgcatc gcgaaccaaa accagtacta 901 gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca 961 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt 1021 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta 1081 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga 1141 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta 1201 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag 1261 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat 1321 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg 1381 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat 1441 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc 1501 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa 1561 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg 1621 gatgaactat acaaacgacg aaaactacgc tgctgtctaa taatactaga gccaggcatc 1681 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1741 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1801 tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 1861 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 1921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 1981 aattgtgagc ggataacaat ttcacacata ctagagaaag aggagaaata ctagatgggc 2041 ccgctgcatc atccggatga tgcgaccctg attagctatg cggcaggcgc actgagccag 2101 gtgattgcgg tggtgaccgc agcgcatctg gaacgttgcg cagaatgccg tgcacgtctg 2161 cgtcaggcag aacagattgg tggcgtgctg atgcagcaga gcattagccg tgtggtgccg 2221 ctgaaaagcc gtatggcgat gctggcacgc ctggatgaac aggaaaccag cgtggatagc 2281 gtgatgcatg cgatgccggc agcgaaccat gatccggatc tgctgccgca ttgcatgcat 2341 gcgcattttg gccgccatct gagcaccctg aaatggaaaa ccctgattcc gggcgtgcag 2401 cgcgtgagcg cacagggcat tgaacagggc aacctgatgc tgctgaaaat tgcaccaggc 2461 gtgagcatgc cggtgcatag ccatgaaagc ggtgaaatga ccatggtgct gaaaggcgcg 2521 tatcatgatg tgcagggtga atttggcctg aacgatgtgg cagatctgga tagccatatt 2581 cagcatcagc cgattgcgta tccggatcgc gaatgcattt gcgtgctggc ggcagaaagc 2641 aaactgcgtt ttcatggctg gatggcacgt atgatgcagc cgttttttgg catttaatac 2701 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2761 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2821 tttctgcgtt tata //