##gff-version 3
##sequence-region BBa_K1461229 1 2834
BBa_K1461229	.	CDS	922	1663	.	+	0	ID=BBa_K1461008;Name=BBa_K1461008
BBa_K1461229	.	ribosome_entry_site	904	915	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1461229	.	conserved_region	908	911	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461229	.	terminator	679	807	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461229	.	terminator	767	807	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	767	807	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461229	.	polyA_site	794	807	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461229	.	stem_loop	774	793	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461229	.	stop_codon	800	800	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461229	.	terminator	679	758	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	679	758	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461229	.	stem_loop	690	733	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461229	.	terminator	1672	1800	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461229	.	terminator	1760	1800	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	1760	1800	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461229	.	polyA_site	1787	1800	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461229	.	stem_loop	1767	1786	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461229	.	stop_codon	1793	1793	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461229	.	terminator	1672	1751	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	1672	1751	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461229	.	stem_loop	1683	1726	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461229	.	engineered_region	1809	2834	.	+	0	ID=BBa_K1461224;Name=BBa_K1461224
BBa_K1461229	.	engineered_region	2017	2834	.	+	0	ID=BBa_K1461207;Name=BBa_K1461207;Parent=BBa_K1461224
BBa_K1461229	.	CDS	2035	2697	.	+	0	ID=BBa_K1461007;Name=BBa_K1461007;Parent=BBa_K1461207
BBa_K1461229	.	ribosome_entry_site	2017	2028	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461207
BBa_K1461229	.	conserved_region	2021	2024	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461229	.	terminator	2706	2834	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461207
BBa_K1461229	.	terminator	2794	2834	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	2794	2834	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461229	.	polyA_site	2821	2834	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461229	.	stem_loop	2801	2820	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461229	.	stop_codon	2827	2827	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461229	.	terminator	2706	2785	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	2706	2785	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461229	.	stem_loop	2717	2760	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461229	.	promoter	1809	2008	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1461224
BBa_K1461229	.	CDS	1809	1896	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1461229	.	promoter	1969	1974	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1461229	.	non_covalent_binding_site	1974	2008	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1461229	.	engineered_region	1809	2008	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1461229	.	promoter	1945	1950	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1461229	.	start_codon	1981	1981	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1461229	.	non_covalent_binding_site	1897	1934	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1461229	.	terminator	2706	2834	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461229	.	terminator	2794	2834	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	2794	2834	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461229	.	polyA_site	2821	2834	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461229	.	stem_loop	2801	2820	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461229	.	stop_codon	2827	2827	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461229	.	terminator	2706	2785	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	2706	2785	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461229	.	stem_loop	2717	2760	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461229	.	promoter	816	895	.	+	0	ID=BBa_K1461002;Name=BBa_K1461002
BBa_K1461229	.	engineered_region	1	807	.	+	0	ID=BBa_K1461212;Name=BBa_K1461212
BBa_K1461229	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1461212
BBa_K1461229	.	engineered_region	44	807	.	+	0	ID=BBa_K1461202;Name=BBa_K1461202;Parent=BBa_K1461212
BBa_K1461229	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461202
BBa_K1461229	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461229	.	CDS	62	670	.	+	0	ID=BBa_K1461005;Name=BBa_K1461005;Parent=BBa_K1461202
BBa_K1461229	.	terminator	679	807	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461202
BBa_K1461229	.	terminator	767	807	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	767	807	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461229	.	polyA_site	794	807	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461229	.	stem_loop	774	793	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461229	.	stop_codon	800	800	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461229	.	terminator	679	758	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461229	.	engineered_region	679	758	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461229	.	stem_loop	690	733	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1461229
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcgcattaccgcgagc
ctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcgg
tggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccgg
cctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagcttt
gatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatc
gcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatag
ccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctat
tttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctgg
cgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaac
acctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcact
ggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtg
atgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcac
tactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatg
cccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttg
aaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcc
ttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcct
tcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtcta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgca
aaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga
gcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttg
tgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctgcatcatcc
ggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaa
cgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtg
tggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgc
gatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctg
aaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaa
ttgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatga
tgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggat
cgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgtttt
ttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata