BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1461202 1 BBa_K1461202 ecf11 (+RBS, +d.term) 2014-09-24T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of RBS (BBa_B0034), ecf11 (BBa_K1461005),and double terminator (BBa_B0015). false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2386280 1 BBa_K1461005 component2386287 1 BBa_B0015 component2386279 1 BBa_B0034 annotation2386280 1 BBa_K1461005 range2386280 1 19 627 annotation2386279 1 BBa_B0034 range2386279 1 1 12 annotation2386287 1 BBa_B0015 range2386287 1 636 764 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1461224 1 BBa_K1461224 anti ecf11 generator (pLac) 2014-09-26T11:00:00Z 2015-05-08T01:10:33Z Assembly. This generator codes anti ecf11 (BBa_K1461007), which prevents ecf11 (BBa_K1461005) from activating the expression of ecf11 promoter (BBa_K1461002). This part works as a reset factor. false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2387514 1 BBa_R0010 component2387531 1 BBa_K1461207 annotation2387531 1 BBa_K1461207 range2387531 1 209 1026 annotation2387514 1 BBa_R0010 range2387514 1 1 200 BBa_K1461229 1 BBa_K1461229 resettable single counter system using ecf11 2014-09-28T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part can be used to characterize the reset-function of our &#963;-Re Counter project. Arabinose-induction causes GFP expression, and IPTG-induction extinguishes the GFP expression. false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2418537 1 BBa_B0034 component2418545 1 BBa_B0015 component2418528 1 BBa_B0015 component2418534 1 BBa_K1461212 component2418538 1 BBa_K1461008 component2418564 1 BBa_K1461224 component2418535 1 BBa_K1461002 component2418558 1 BBa_B0015 annotation2418534 1 BBa_K1461212 range2418534 1 1 807 annotation2418537 1 BBa_B0034 range2418537 1 904 915 annotation2418564 1 BBa_K1461224 range2418564 1 1809 2834 annotation2418528 1 BBa_B0015 range2418528 1 679 807 annotation2418535 1 BBa_K1461002 range2418535 1 816 895 annotation2418538 1 BBa_K1461008 range2418538 1 922 1663 annotation2418545 1 BBa_B0015 range2418545 1 1672 1800 annotation2418558 1 BBa_B0015 range2418558 1 2706 2834 BBa_K1461007 1 BBa_K1461007 anti ecf11 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Syringae. This part is from Pseudomonas Syringae RpoE. ecf11 is a sigma factor which activates ecf11 promoter (BBa_K1461002) false false _1840_ 0 18036 9 It's complicated true None. false Takefumi Yoshikawa BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K1461002 1 BBa_K1461002 ecf11 promoter 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Syringae. This part is from Pseudomonas Syringae RpoE. ecf20 (BBa_K1461005) activates the expression of the downstream genes of this promoter. false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1461005 1 BBa_K1461005 ecf11 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Syringae. This part is from Pseudomonas Syringae RpoE. ecf11 is a sigma factor which activates ecf11 promoter (BBa_K1461002) false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1461207 1 BBa_K1461207 anti ecf11 (+RBS, +d.term) 2014-09-25T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of RBS (BBa_B0034), anti ecf20 (BBa_K1461007), and double terminator (BBa_B0015). false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2386625 1 BBa_K1461007 component2386632 1 BBa_B0015 component2386624 1 BBa_B0034 annotation2386625 1 BBa_K1461007 range2386625 1 19 681 annotation2386624 1 BBa_B0034 range2386624 1 1 12 annotation2386632 1 BBa_B0015 range2386632 1 690 818 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_K1461212 1 BBa_K1461212 constitutive ecf11 generator 2014-09-25T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is arabinose-induced ecf11 generator. false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2401084 1 BBa_J23101 component2401095 1 BBa_K1461202 annotation2401095 1 BBa_K1461202 range2401095 1 44 807 annotation2401084 1 BBa_J23101 range2401084 1 1 35 BBa_K1461008 1 BBa_K1461008 GFP (AAV tag +) 2014-10-12T11:00:00Z 2015-05-08T01:10:33Z PCR. This part is GFP(mut3b) (BBa_E0040) with an AAV degradation tag added by PCR. false false _1840_ 0 18036 9 Not in stock false None. false Takefumi Yoshikawa BBa_K1461005_sequence 1 atgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaa BBa_K1461212_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1461007_sequence 1 atgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1461229_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461202_sequence 1 aaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1461002_sequence 1 gcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccag BBa_K1461207_sequence 1 aaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461008_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataa BBa_K1461224_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z