LOCUS BBa_K1461230 2929 bp DNA linear 16-Dec-2024 DEFINITION reset function using ecf11 ACCESSION BBa_K1461230 VERSION BBa_K1461230.1 FEATURES Location/Qualifiers stem_loop 785..828 /label=stem_loop misc_feature 774..853 /label=BBa_B0010 terminator 774..853 /label=BBa_B0010 stem_loop 869..888 /label=T7 TE misc_feature 895..895 /label=stop polyA_site 889..902 /label=polya misc_feature 862..902 /label=BBa_B0012 terminator 862..902 /label=BBa_B0012 terminator 774..902 /label=BBa_B0015 promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 157..765 /label=BBa_K1461005 stem_loop 785..828 /label=stem_loop misc_feature 774..853 /label=BBa_B0010 terminator 774..853 /label=BBa_B0010 stem_loop 869..888 /label=T7 TE misc_feature 895..895 /label=stop polyA_site 889..902 /label=polya misc_feature 862..902 /label=BBa_B0012 terminator 862..902 /label=BBa_B0012 terminator 774..902 /label=BBa_B0015 misc_feature 139..902 /label=BBa_K1461202 misc_feature 1..902 /label=BBa_K1461211 promoter 911..990 /label=BBa_K1461002 misc_feature 1003..1006 /label=conserved RBS 999..1010 /label=BBa_B0034 CDS 1017..1758 /label=BBa_K1461008 stem_loop 1778..1821 /label=stem_loop misc_feature 1767..1846 /label=BBa_B0010 terminator 1767..1846 /label=BBa_B0010 stem_loop 1862..1881 /label=T7 TE misc_feature 1888..1888 /label=stop polyA_site 1882..1895 /label=polya misc_feature 1855..1895 /label=BBa_B0012 terminator 1855..1895 /label=BBa_B0012 terminator 1767..1895 /label=BBa_B0015 stem_loop 2812..2855 /label=stem_loop misc_feature 2801..2880 /label=BBa_B0010 terminator 2801..2880 /label=BBa_B0010 stem_loop 2896..2915 /label=T7 TE misc_feature 2922..2922 /label=stop polyA_site 2916..2929 /label=polya misc_feature 2889..2929 /label=BBa_B0012 terminator 2889..2929 /label=BBa_B0012 terminator 2801..2929 /label=BBa_B0015 CDS 1904..1991 /label=end of LacI coding region (inactive) misc_feature 1904..2103 /label=BBa_R0010 misc_feature 1992..2029 /label=CAP binding site promoter 2040..2045 /label=-35 promoter 2064..2069 /label=-10 misc_feature 2069..2103 /label=LacI binding site misc_feature 2076..2076 /label=start promoter 1904..2103 /label=BBa_R0010 misc_feature 2116..2119 /label=conserved RBS 2112..2123 /label=BBa_B0034 CDS 2130..2792 /label=BBa_K1461007 stem_loop 2812..2855 /label=stem_loop misc_feature 2801..2880 /label=BBa_B0010 terminator 2801..2880 /label=BBa_B0010 stem_loop 2896..2915 /label=T7 TE misc_feature 2922..2922 /label=stop polyA_site 2916..2929 /label=polya misc_feature 2889..2929 /label=BBa_B0012 terminator 2889..2929 /label=BBa_B0012 terminator 2801..2929 /label=BBa_B0015 misc_feature 2112..2929 /label=BBa_K1461207 misc_feature 1904..2929 /label=BBa_K1461224 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatgc gcattaccgc gagcctgcgc 181 accttttgcc atctgagcac cccgcatagc gatagcacca ccagccgcct gtggattgat 241 gaagtgaccg cggtggcgcg ccagcgcgat cgcgatagct ttatgcgcat ttatgatcat 301 tttgcgccgc gcctgctgcg ctatctgacc ggcctgaacg tgccggaagg ccaggcggaa 361 gaactggtgc aggaagtgct gctgaaactg tggcataaag cggaaagctt tgatccgagc 421 aaagcgagcc tgggcacctg gctgtttcgc attgcgcgca acctgtatat tgatagcgtg 481 cgcaaagatc gcggctgggt gcaggtgcag aacagcctgg aacagctgga acgcctggaa 541 gcgccggtgg atcgcaccct ggattatagc cagcgccagg aacagcagct gaacagcgcg 601 attcagaacc tgccgaccga tcaggcgaaa gtgctgcgca tgagctattt tgaagcgctg 661 agccatcgcg aaattagcga acgcctggat atgccgctgg gcaccgtgaa aagctgcctg 721 cgcctggcgt ttcagaaact gcgcagccgc attgaagaaa gctaatacta gagccaggca 781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 901 tatactagag gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg 961 taacacctct gcatcgcgaa ccaaaaccag tactagagaa agaggagaaa tactagatgc 1021 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg 1081 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 1141 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca 1201 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg 1261 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag 1321 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 1381 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg 1441 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 1501 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt 1561 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 1621 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 1681 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa cgacgaaaac 1741 tacgctgctg tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 1801 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 1861 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcaatacg caaaccgcct 1921 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 1981 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 2041 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 2101 acatactaga gaaagaggag aaatactaga tgggcccgct gcatcatccg gatgatgcga 2161 ccctgattag ctatgcggca ggcgcactga gccaggtgat tgcggtggtg accgcagcgc 2221 atctggaacg ttgcgcagaa tgccgtgcac gtctgcgtca ggcagaacag attggtggcg 2281 tgctgatgca gcagagcatt agccgtgtgg tgccgctgaa aagccgtatg gcgatgctgg 2341 cacgcctgga tgaacaggaa accagcgtgg atagcgtgat gcatgcgatg ccggcagcga 2401 accatgatcc ggatctgctg ccgcattgca tgcatgcgca ttttggccgc catctgagca 2461 ccctgaaatg gaaaaccctg attccgggcg tgcagcgcgt gagcgcacag ggcattgaac 2521 agggcaacct gatgctgctg aaaattgcac caggcgtgag catgccggtg catagccatg 2581 aaagcggtga aatgaccatg gtgctgaaag gcgcgtatca tgatgtgcag ggtgaatttg 2641 gcctgaacga tgtggcagat ctggatagcc atattcagca tcagccgatt gcgtatccgg 2701 atcgcgaatg catttgcgtg ctggcggcag aaagcaaact gcgttttcat ggctggatgg 2761 cacgtatgat gcagccgttt tttggcattt aatactagag ccaggcatca aataaaacga 2821 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2881 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //