##gff-version 3
##sequence-region BBa_K1461230 1 2929
BBa_K1461230	.	promoter	911	990	.	+	0	ID=BBa_K1461002;Name=BBa_K1461002
BBa_K1461230	.	terminator	1767	1895	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461230	.	terminator	1767	1846	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	1778	1821	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461230	.	engineered_region	1767	1846	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461230	.	terminator	1855	1895	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	1862	1881	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461230	.	stop_codon	1888	1888	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461230	.	polyA_site	1882	1895	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461230	.	engineered_region	1855	1895	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461230	.	CDS	1017	1758	.	+	0	ID=BBa_K1461008;Name=BBa_K1461008
BBa_K1461230	.	engineered_region	1	902	.	+	0	ID=BBa_K1461211;Name=BBa_K1461211
BBa_K1461230	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K1461211
BBa_K1461230	.	engineered_region	139	902	.	+	0	ID=BBa_K1461202;Name=BBa_K1461202;Parent=BBa_K1461211
BBa_K1461230	.	terminator	774	902	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461202
BBa_K1461230	.	terminator	774	853	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	785	828	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461230	.	engineered_region	774	853	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461230	.	terminator	862	902	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	869	888	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461230	.	stop_codon	895	895	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461230	.	polyA_site	889	902	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461230	.	engineered_region	862	902	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461230	.	CDS	157	765	.	+	0	ID=BBa_K1461005;Name=BBa_K1461005;Parent=BBa_K1461202
BBa_K1461230	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461202
BBa_K1461230	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461230	.	terminator	774	902	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461230	.	terminator	774	853	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	785	828	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461230	.	engineered_region	774	853	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461230	.	terminator	862	902	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	869	888	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461230	.	stop_codon	895	895	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461230	.	polyA_site	889	902	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461230	.	engineered_region	862	902	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461230	.	terminator	2801	2929	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461230	.	terminator	2801	2880	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	2812	2855	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461230	.	engineered_region	2801	2880	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461230	.	terminator	2889	2929	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	2896	2915	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461230	.	stop_codon	2922	2922	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461230	.	polyA_site	2916	2929	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461230	.	engineered_region	2889	2929	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461230	.	ribosome_entry_site	999	1010	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1461230	.	conserved_region	1003	1006	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461230	.	engineered_region	1904	2929	.	+	0	ID=BBa_K1461224;Name=BBa_K1461224
BBa_K1461230	.	promoter	1904	2103	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1461224
BBa_K1461230	.	engineered_region	1904	2103	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1461230	.	CDS	1904	1991	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1461230	.	promoter	2064	2069	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1461230	.	start_codon	2076	2076	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1461230	.	non_covalent_binding_site	2069	2103	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1461230	.	non_covalent_binding_site	1992	2029	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1461230	.	promoter	2040	2045	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1461230	.	engineered_region	2112	2929	.	+	0	ID=BBa_K1461207;Name=BBa_K1461207;Parent=BBa_K1461224
BBa_K1461230	.	ribosome_entry_site	2112	2123	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461207
BBa_K1461230	.	conserved_region	2116	2119	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461230	.	terminator	2801	2929	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461207
BBa_K1461230	.	terminator	2801	2880	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	2812	2855	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461230	.	engineered_region	2801	2880	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461230	.	terminator	2889	2929	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461230	.	stem_loop	2896	2915	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461230	.	stop_codon	2922	2922	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461230	.	polyA_site	2916	2929	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461230	.	engineered_region	2889	2929	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461230	.	CDS	2130	2792	.	+	0	ID=BBa_K1461007;Name=BBa_K1461007;Parent=BBa_K1461207
>BBa_K1461230
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggatt
gatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgc
tgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggca
taaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgat
agcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatc
gcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagt
gctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaa
agctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactctt
catcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgtaaagg
agaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagt
ggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccat
ggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactt
tttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgt
gctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaaca
ttcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaa
agttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaatt
ggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaaga
gagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaa
actacgctgctgtctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgg
gcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgac
cgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcag
cagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtgg
atagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccg
ccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaac
ctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctga
aaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagcc
gattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgt
atgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata