BBa_K1461230
type: ComponentDefinition
persistent identity: BBa K1461230
display id: BBa_K1461230
version: 1
was generated by: iGEM to SBOL conversion
description: reset function using ecf11
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This part constitutively codes GFP. IPTG-induction extinguishes GFP expression, coding anti ecf11 (BBa_K1461007), which prevents the interaction of ecf11 (BBa_K1461005) and ecf11 promoter (BBa_K1461002).
discontinued: false
dominant: false
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_K1461224
component: BBa_B0015
component: BBa_K1461002
component: BBa_K1461211
component: BBa_K1461008
component: BBa_B0034
sequence annotation: BBa_K1461211
sequence annotation: BBa_B0015
sequence annotation: BBa_K1461002
sequence annotation: BBa_B0034
sequence annotation: BBa_K1461008
sequence annotation: BBa_K1461224
sequence: https://synbiohub.org/public/igem/BBa_K1461230_sequence/1

BBa_K1461224
type: Component
persistent identity: component2418607
display id: component2418607
version: 1
top level: BBa_K1461230
definition: BBa_K1461224
access: public

BBa_B0015
type: Component
persistent identity: component2418570
display id: component2418570
version: 1
top level: BBa_K1461230
definition: BBa_B0015
access: public

BBa_K1461002
type: Component
persistent identity: component2418578
display id: component2418578
version: 1
top level: BBa_K1461230
definition: BBa_K1461002
access: public

BBa_K1461211
type: Component
persistent identity: component2418577
display id: component2418577
version: 1
top level: BBa_K1461230
definition: BBa_K1461211
access: public

BBa_K1461008
type: Component
persistent identity: component2418581
display id: component2418581
version: 1
top level: BBa_K1461230
definition: BBa_K1461008
access: public

BBa_B0034
type: Component
persistent identity: component2418580
display id: component2418580
version: 1
top level: BBa_K1461230
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2418601
display id: component2418601
version: 1
top level: BBa_K1461230
definition: BBa_B0015
access: public

BBa_B0015
type: Component
persistent identity: component2418588
display id: component2418588
version: 1
top level: BBa_K1461230
definition: BBa_B0015
access: public

BBa_K1461211
type: SequenceAnnotation
persistent identity: annotation2418577
display id: annotation2418577
version: 1
top level: BBa_K1461230
location: https://synbiohub.org/public/igem/BBa_K1461230/annotation2418577/range2418577/1
component: BBa_K1461211

https://synbiohub.org/public/igem/BBa_K1461230/annotation2418577/range2418577/1
type: Range
persistent identity: range2418577
display id: range2418577
version: 1
top level: BBa_K1461230
direction: direction/reverse
start: 1
end: 902
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2418570
display id: annotation2418570
version: 1
top level: BBa_K1461230
location: https://synbiohub.org/public/igem/BBa_K1461230/annotation2418570/range2418570/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461230/annotation2418570/range2418570/1
type: Range
persistent identity: range2418570
display id: range2418570
version: 1
top level: BBa_K1461230
direction: direction/reverse
start: 774
end: 902
orientation: inline

BBa_K1461002
type: SequenceAnnotation
persistent identity: annotation2418578
display id: annotation2418578
version: 1
top level: BBa_K1461230
location: https://synbiohub.org/public/igem/BBa_K1461230/annotation2418578/range2418578/1
component: BBa_K1461002

https://synbiohub.org/public/igem/BBa_K1461230/annotation2418578/range2418578/1
type: Range
persistent identity: range2418578
display id: range2418578
version: 1
top level: BBa_K1461230
direction: direction/reverse
start: 911
end: 990
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418580
display id: annotation2418580
version: 1
top level: BBa_K1461230
location: https://synbiohub.org/public/igem/BBa_K1461230/annotation2418580/range2418580/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1461230/annotation2418580/range2418580/1
type: Range
persistent identity: range2418580
display id: range2418580
version: 1
top level: BBa_K1461230
direction: direction/reverse
start: 999
end: 1010
orientation: inline

BBa_K1461008
type: SequenceAnnotation
persistent identity: annotation2418581
display id: annotation2418581
version: 1
top level: BBa_K1461230
location: https://synbiohub.org/public/igem/BBa_K1461230/annotation2418581/range2418581/1
component: BBa_K1461008

https://synbiohub.org/public/igem/BBa_K1461230/annotation2418581/range2418581/1
type: Range
persistent identity: range2418581
display id: range2418581
version: 1
top level: BBa_K1461230
direction: direction/reverse
start: 1017
end: 1758
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2418588
display id: annotation2418588
version: 1
top level: BBa_K1461230
location: https://synbiohub.org/public/igem/BBa_K1461230/annotation2418588/range2418588/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461230/annotation2418588/range2418588/1
type: Range
persistent identity: range2418588
display id: range2418588
version: 1
top level: BBa_K1461230
direction: direction/reverse
start: 1767
end: 1895
orientation: inline

BBa_K1461224
type: SequenceAnnotation
persistent identity: annotation2418607
display id: annotation2418607
version: 1
top level: BBa_K1461230
location: https://synbiohub.org/public/igem/BBa_K1461230/annotation2418607/range2418607/1
component: BBa_K1461224

https://synbiohub.org/public/igem/BBa_K1461230/annotation2418607/range2418607/1
type: Range
persistent identity: range2418607
display id: range2418607
version: 1
top level: BBa_K1461230
direction: direction/reverse
start: 1904
end: 2929
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2418601
display id: annotation2418601
version: 1
top level: BBa_K1461230
location: https://synbiohub.org/public/igem/BBa_K1461230/annotation2418601/range2418601/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461230/annotation2418601/range2418601/1
type: Range
persistent identity: range2418601
display id: range2418601
version: 1
top level: BBa_K1461230
direction: direction/reverse
start: 2801
end: 2929
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1461002
type: ComponentDefinition
persistent identity: BBa K1461002
display id: BBa_K1461002
version: 1
was generated by: iGEM to SBOL conversion
description: ecf11 promoter
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Pseudomonas Syringae.
owned by: james
owned by: myers
mutable description: This part is from Pseudomonas Syringae RpoE. ecf20 (BBa_K1461005) activates the expression of the downstream genes of this promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1461002_sequence/1

BBa_K1461211
type: ComponentDefinition
persistent identity: BBa K1461211
display id: BBa_K1461211
version: 1
was generated by: iGEM to SBOL conversion
description: arabinose-induced ecf11 generator
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This generator codes ecf11, a sigma factor, constitutively.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_I13453
component: BBa_K1461202
sequence annotation: BBa_I13453
sequence annotation: BBa_K1461202
sequence: https://synbiohub.org/public/igem/BBa_K1461211_sequence/1

BBa_I13453
type: Component
persistent identity: component2401072
display id: component2401072
version: 1
top level: BBa_K1461211
definition: BBa_I13453
access: public

BBa_K1461202
type: Component
persistent identity: component2401083
display id: component2401083
version: 1
top level: BBa_K1461211
definition: BBa_K1461202
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2401072
display id: annotation2401072
version: 1
top level: BBa_K1461211
location: https://synbiohub.org/public/igem/BBa_K1461211/annotation2401072/range2401072/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1461211/annotation2401072/range2401072/1
type: Range
persistent identity: range2401072
display id: range2401072
version: 1
top level: BBa_K1461211
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_K1461202
type: SequenceAnnotation
persistent identity: annotation2401083
display id: annotation2401083
version: 1
top level: BBa_K1461211
location: https://synbiohub.org/public/igem/BBa_K1461211/annotation2401083/range2401083/1
component: BBa_K1461202

https://synbiohub.org/public/igem/BBa_K1461211/annotation2401083/range2401083/1
type: Range
persistent identity: range2401083
display id: range2401083
version: 1
top level: BBa_K1461211
direction: direction/reverse
start: 139
end: 902
orientation: inline

BBa_K1461224
type: ComponentDefinition
persistent identity: BBa K1461224
display id: BBa_K1461224
version: 1
was generated by: iGEM to SBOL conversion
description: anti ecf11 generator (pLac)
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This generator codes anti ecf11 (BBa_K1461007), which prevents ecf11 (BBa_K1461005) from activating the expression of ecf11 promoter (BBa_K1461002). This part works as a reset factor.
discontinued: false
dominant: false
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_R0010
component: BBa_K1461207
sequence annotation: BBa_R0010
sequence annotation: BBa_K1461207
sequence: https://synbiohub.org/public/igem/BBa_K1461224_sequence/1

BBa_R0010
type: Component
persistent identity: component2387514
display id: component2387514
version: 1
top level: BBa_K1461224
definition: LacI
access: public

BBa_K1461207
type: Component
persistent identity: component2387531
display id: component2387531
version: 1
top level: BBa_K1461224
definition: BBa_K1461207
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2387514
display id: annotation2387514
version: 1
top level: BBa_K1461224
location: https://synbiohub.org/public/igem/BBa_K1461224/annotation2387514/range2387514/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1461224/annotation2387514/range2387514/1
type: Range
persistent identity: range2387514
display id: range2387514
version: 1
top level: BBa_K1461224
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1461207
type: SequenceAnnotation
persistent identity: annotation2387531
display id: annotation2387531
version: 1
top level: BBa_K1461224
location: https://synbiohub.org/public/igem/BBa_K1461224/annotation2387531/range2387531/1
component: BBa_K1461207

https://synbiohub.org/public/igem/BBa_K1461224/annotation2387531/range2387531/1
type: Range
persistent identity: range2387531
display id: range2387531
version: 1
top level: BBa_K1461224
direction: direction/reverse
start: 209
end: 1026
orientation: inline

BBa_K1461008
type: ComponentDefinition
persistent identity: BBa K1461008
display id: BBa_K1461008
version: 1
was generated by: iGEM to SBOL conversion
description: GFP (AAV tag +)
created: 2014-10-12T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: PCR.
owned by: james
owned by: myers
mutable description: This part is GFP(mut3b) (BBa_E0040) with an AAV degradation tag added by PCR.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1461008_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K1461202
type: ComponentDefinition
persistent identity: BBa K1461202
display id: BBa_K1461202
version: 1
was generated by: iGEM to SBOL conversion
description: ecf11 (+RBS, +d.term)
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This part is a composite part of RBS (BBa_B0034), ecf11 (BBa_K1461005),and double terminator (BBa_B0015).
discontinued: false
dominant: false
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K1461005
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1461005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1461202_sequence/1

BBa_B0015
type: Component
persistent identity: component2386287
display id: component2386287
version: 1
top level: BBa_K1461202
definition: BBa_B0015
access: public

BBa_K1461005
type: Component
persistent identity: component2386280
display id: component2386280
version: 1
top level: BBa_K1461202
definition: BBa_K1461005
access: public

BBa_B0034
type: Component
persistent identity: component2386279
display id: component2386279
version: 1
top level: BBa_K1461202
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386279
display id: annotation2386279
version: 1
top level: BBa_K1461202
location: https://synbiohub.org/public/igem/BBa_K1461202/annotation2386279/range2386279/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1461202/annotation2386279/range2386279/1
type: Range
persistent identity: range2386279
display id: range2386279
version: 1
top level: BBa_K1461202
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1461005
type: SequenceAnnotation
persistent identity: annotation2386280
display id: annotation2386280
version: 1
top level: BBa_K1461202
location: https://synbiohub.org/public/igem/BBa_K1461202/annotation2386280/range2386280/1
component: BBa_K1461005

https://synbiohub.org/public/igem/BBa_K1461202/annotation2386280/range2386280/1
type: Range
persistent identity: range2386280
display id: range2386280
version: 1
top level: BBa_K1461202
direction: direction/reverse
start: 19
end: 627
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386287
display id: annotation2386287
version: 1
top level: BBa_K1461202
location: https://synbiohub.org/public/igem/BBa_K1461202/annotation2386287/range2386287/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461202/annotation2386287/range2386287/1
type: Range
persistent identity: range2386287
display id: range2386287
version: 1
top level: BBa_K1461202
direction: direction/reverse
start: 636
end: 764
orientation: inline

BBa_K1461207
type: ComponentDefinition
persistent identity: BBa K1461207
display id: BBa_K1461207
version: 1
was generated by: iGEM to SBOL conversion
description: anti ecf11 (+RBS, +d.term)
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This part is a composite part of RBS (BBa_B0034), anti ecf20 (BBa_K1461007), and double terminator (BBa_B0015).
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K1461007
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1461007
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1461207_sequence/1

BBa_B0015
type: Component
persistent identity: component2386632
display id: component2386632
version: 1
top level: BBa_K1461207
definition: BBa_B0015
access: public

BBa_K1461007
type: Component
persistent identity: component2386625
display id: component2386625
version: 1
top level: BBa_K1461207
definition: BBa_K1461007
access: public

BBa_B0034
type: Component
persistent identity: component2386624
display id: component2386624
version: 1
top level: BBa_K1461207
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386624
display id: annotation2386624
version: 1
top level: BBa_K1461207
location: https://synbiohub.org/public/igem/BBa_K1461207/annotation2386624/range2386624/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1461207/annotation2386624/range2386624/1
type: Range
persistent identity: range2386624
display id: range2386624
version: 1
top level: BBa_K1461207
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1461007
type: SequenceAnnotation
persistent identity: annotation2386625
display id: annotation2386625
version: 1
top level: BBa_K1461207
location: https://synbiohub.org/public/igem/BBa_K1461207/annotation2386625/range2386625/1
component: BBa_K1461007

https://synbiohub.org/public/igem/BBa_K1461207/annotation2386625/range2386625/1
type: Range
persistent identity: range2386625
display id: range2386625
version: 1
top level: BBa_K1461207
direction: direction/reverse
start: 19
end: 681
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386632
display id: annotation2386632
version: 1
top level: BBa_K1461207
location: https://synbiohub.org/public/igem/BBa_K1461207/annotation2386632/range2386632/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461207/annotation2386632/range2386632/1
type: Range
persistent identity: range2386632
display id: range2386632
version: 1
top level: BBa_K1461207
direction: direction/reverse
start: 690
end: 818
orientation: inline

BBa_K1461007
type: ComponentDefinition
persistent identity: BBa K1461007
display id: BBa_K1461007
version: 1
was generated by: iGEM to SBOL conversion
description: anti ecf11
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Pseudomonas Syringae.
owned by: james
owned by: myers
mutable description: This part is from Pseudomonas Syringae RpoE. ecf11 is a sigma factor which activates ecf11 promoter (BBa_K1461002)
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1461007_sequence/1

BBa_K1461005
type: ComponentDefinition
persistent identity: BBa K1461005
display id: BBa_K1461005
version: 1
was generated by: iGEM to SBOL conversion
description: ecf11
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Pseudomonas Syringae.
owned by: james
owned by: myers
mutable description: This part is from Pseudomonas Syringae RpoE. ecf11 is a sigma factor which activates ecf11 promoter (BBa_K1461002)
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1461005_sequence/1

https://synbiohub.org/public/igem/BBa_K1461230_sequence/1
type: Sequence
persistent identity: BBa K1461230 sequence
display id: BBa_K1461230_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461002_sequence/1
type: Sequence
persistent identity: BBa K1461002 sequence
display id: BBa_K1461002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461211_sequence/1
type: Sequence
persistent identity: BBa K1461211 sequence
display id: BBa_K1461211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461224_sequence/1
type: Sequence
persistent identity: BBa K1461224 sequence
display id: BBa_K1461224_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461008_sequence/1
type: Sequence
persistent identity: BBa K1461008 sequence
display id: BBa_K1461008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461202_sequence/1
type: Sequence
persistent identity: BBa K1461202 sequence
display id: BBa_K1461202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461207_sequence/1
type: Sequence
persistent identity: BBa K1461207 sequence
display id: BBa_K1461207_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461007_sequence/1
type: Sequence
persistent identity: BBa K1461007 sequence
display id: BBa_K1461007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461005_sequence/1
type: Sequence
persistent identity: BBa K1461005 sequence
display id: BBa_K1461005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z