This device generates mRNA coding GFP whose RBS (ribosome binding site) is covered with cis-repressor (cr) sequence. Arabinose-induction expresses taRNA (BBa_K1461003), which recovers the RBS from crRBS and activates the expression of GFP. This system is basic way to count in our σ-Re Counter project.
This part is arabinose-induced taRNA generator. This part works as a trigger of σ-ReCounter.
This part codes GFP, but the RBS of the transcripted mRNA is covered with cis-repressor (cr) sequence and the GFP coding sequence is not translated. taRNA (BBa_K1461003) recover the RBS and activates translation.
This part is a composite part of taRNA (BBa_K1461003) and double terminator (BBa_B0015).
This part is a composite part of crRBS (BBa_K1461000), GFP (BBa_E0040), double terminator (BBa_B0015). With promoter, mRNA which codes GFP will be transcripted, but not usually translated. taRNA (BBa_K1461003) activates the translation.
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
Double terminator consisting of BBa_B0010 and BBa_B0012
This part is ribosome-binding site (RBS) with cis-repressor (cr) sequence. The cis-repressor sequence is placed between the transcription start site and the RBS, and its complementarity with the RBS causes a stem-loop structure to form upon transcription. This secondary structure prevents binding of the 30S ribosomal subunit to the RBS, which inhibits translation.
This part hybridizes to crRBS (BBa_K1461000) and promotes the translation of the downstream genes of crRBS.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1