LOCUS BBa_K1461235 3482 bp DNA linear 28-Jun-2024 DEFINITION resettable counter device using ecf20 (w/o crRBS, taRNA) ACCESSION BBa_K1461235 VERSION BBa_K1461235.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 157..738 /label=BBa_K1461004 stem_loop 758..801 /label=stem_loop misc_feature 747..826 /label=BBa_B0010 terminator 747..826 /label=BBa_B0010 stem_loop 842..861 /label=T7 TE misc_feature 868..868 /label=stop polyA_site 862..875 /label=polya misc_feature 835..875 /label=BBa_B0012 terminator 835..875 /label=BBa_B0012 terminator 747..875 /label=BBa_B0015 misc_feature 139..875 /label=BBa_K1461201 misc_feature 1..875 /label=BBa_K1461209 promoter 884..963 /label=BBa_K1461001 misc_feature 976..979 /label=conserved RBS 972..983 /label=BBa_B0034 CDS 990..1709 /label=GFP protein CDS 990..1709 /label=BBa_E0040 stem_loop 1729..1772 /label=stem_loop misc_feature 1718..1797 /label=BBa_B0010 terminator 1718..1797 /label=BBa_B0010 stem_loop 1813..1832 /label=T7 TE misc_feature 1839..1839 /label=stop polyA_site 1833..1846 /label=polya misc_feature 1806..1846 /label=BBa_B0012 terminator 1806..1846 /label=BBa_B0012 terminator 1718..1846 /label=BBa_B0015 misc_feature 884..1846 /label=BBa_K1461219 misc_feature 1..1846 /label=BBa_K1461225 promoter 1855..1934 /label=BBa_K1461001 misc_feature 1947..1950 /label=conserved RBS 1943..1954 /label=BBa_B0034 CDS 1961..2542 /label=BBa_K1461004 stem_loop 2562..2605 /label=stem_loop misc_feature 2551..2630 /label=BBa_B0010 terminator 2551..2630 /label=BBa_B0010 stem_loop 2646..2665 /label=T7 TE misc_feature 2672..2672 /label=stop polyA_site 2666..2679 /label=polya misc_feature 2639..2679 /label=BBa_B0012 terminator 2639..2679 /label=BBa_B0012 terminator 2551..2679 /label=BBa_B0015 misc_feature 1855..2679 /label=BBa_K1461221 misc_feature 1..2679 /label=BBa_K1461233 CDS 2688..2775 /label=end of LacI coding region (inactive) misc_feature 2688..2887 /label=BBa_R0010 misc_feature 2776..2813 /label=CAP binding site promoter 2824..2829 /label=-35 promoter 2848..2853 /label=-10 misc_feature 2853..2887 /label=LacI binding site misc_feature 2860..2860 /label=start promoter 2688..2887 /label=BBa_R0010 misc_feature 2900..2903 /label=conserved RBS 2896..2907 /label=BBa_B0034 CDS 2914..3345 /label=BBa_K1461006 stem_loop 3365..3408 /label=stem_loop misc_feature 3354..3433 /label=BBa_B0010 terminator 3354..3433 /label=BBa_B0010 stem_loop 3449..3468 /label=T7 TE misc_feature 3475..3475 /label=stop polyA_site 3469..3482 /label=polya misc_feature 3442..3482 /label=BBa_B0012 terminator 3442..3482 /label=BBa_B0012 terminator 3354..3482 /label=BBa_B0015 misc_feature 2896..3482 /label=BBa_K1461206 misc_feature 2688..3482 /label=BBa_K1461223 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga acgaaaccga tccggatctg 181 gaactgctga aacgtattgg caacaacgat gcacaggcgg tgaaagaaat ggtgacccgc 241 aaactgccgc gcctgctggc gctggcgagc cgtctgctgg gcgatgcgga tgaagcgcgc 301 gatattgcgc aggaaagctt tctgcgcatt tggaaacagg cagcgagctg gcgcagcgaa 361 caggcgcgct ttgatacctg gctgcatcgc gtggcgctga acctgtgcta tgatcgcctg 421 cgccgtcgta aagaacatgt gccggtggat agcgaacatg cgtgcgaagc gctggatacc 481 cgcccggcgc cggatgaaca gctggaagcg agcgcgcaga gccgtcgcat ggcgcaggca 541 ctggatcagc tgccggatcg tcagcgcgaa gcgattgtgc tgcaatatta tcaggaactg 601 agcaacaccg aagcagcggc actgatgcag attagcgtgg aagcgctgga aagcctgctg 661 agccgcgcac gtcgcaacct gcgcagccat ctggcggaag cgccgggtgc ggatctgagc 721 ggccgtcgca aaccgtaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggcgcgga taaaaatttc 901 atttgcccgc gacggattcc ccgcccatct atcgttgaac ccatcagctg cgttcatcag 961 cgatactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag 1021 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg 1081 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg 1141 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct 1201 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag 1261 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg 1321 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta 1381 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat 1441 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca 1501 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg 1561 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc 1621 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac 1681 atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag 1741 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 1801 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agaggcgcgg 1861 ataaaaattt catttgcccg cgacggattc cccgcccatc tatcgttgaa cccatcagct 1921 gcgttcatca gcgatactag agaaagagga gaaatactag atgaacgaaa ccgatccgga 1981 tctggaactg ctgaaacgta ttggcaacaa cgatgcacag gcggtgaaag aaatggtgac 2041 ccgcaaactg ccgcgcctgc tggcgctggc gagccgtctg ctgggcgatg cggatgaagc 2101 gcgcgatatt gcgcaggaaa gctttctgcg catttggaaa caggcagcga gctggcgcag 2161 cgaacaggcg cgctttgata cctggctgca tcgcgtggcg ctgaacctgt gctatgatcg 2221 cctgcgccgt cgtaaagaac atgtgccggt ggatagcgaa catgcgtgcg aagcgctgga 2281 tacccgcccg gcgccggatg aacagctgga agcgagcgcg cagagccgtc gcatggcgca 2341 ggcactggat cagctgccgg atcgtcagcg cgaagcgatt gtgctgcaat attatcagga 2401 actgagcaac accgaagcag cggcactgat gcagattagc gtggaagcgc tggaaagcct 2461 gctgagccgc gcacgtcgca acctgcgcag ccatctggcg gaagcgccgg gtgcggatct 2521 gagcggccgt cgcaaaccgt aatactagag ccaggcatca aataaaacga aaggctcagt 2581 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 2641 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc 2701 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 2761 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 2821 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 2881 tcacacatac tagagaaaga ggagaaatac tagatgaccc cggaacgttt tgtgcatctg 2941 gcggatgcgt atggtgcaga tctgcaacgt tggccgagcg cagaacgtgc agcagcacag 3001 gcactgctgg attgcggcga tgcgcaggca gtggcagcgc tgcgtcaggc acattggctg 3061 gatagccagc tggatcgtta tcaggtgccg gcaccgagcc cggcactggc acagcgtatt 3121 attgcggcag cacaacagcc gggtgcaccg ttttggagcc gctatgcagg ttggctggca 3181 agcctgggtt gggtgggtgt gggcctgaca ggcgtggcag cgggtatgct ggcggtggca 3241 ctgagcctgc cattaagcac cagcgcagaa gcactgccaa gcgtgtttga tcagagcgat 3301 gcagaatttg tgctgagcat taacgcggaa gaagcggaac agtaatacta gagccaggca 3361 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 3421 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 3481 ta //