##gff-version 3
##sequence-region BBa_K1461235 1 3482
BBa_K1461235	.	engineered_region	2688	3482	.	+	0	ID=BBa_K1461223;Name=BBa_K1461223
BBa_K1461235	.	engineered_region	2896	3482	.	+	0	ID=BBa_K1461206;Name=BBa_K1461206;Parent=BBa_K1461223
BBa_K1461235	.	CDS	2914	3345	.	+	0	ID=BBa_K1461006;Name=BBa_K1461006;Parent=BBa_K1461206
BBa_K1461235	.	ribosome_entry_site	2896	2907	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461206
BBa_K1461235	.	conserved_region	2900	2903	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461235	.	terminator	3354	3482	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461206
BBa_K1461235	.	terminator	3354	3433	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461235	.	engineered_region	3354	3433	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461235	.	stem_loop	3365	3408	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461235	.	terminator	3442	3482	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461235	.	stop_codon	3475	3475	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461235	.	polyA_site	3469	3482	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461235	.	stem_loop	3449	3468	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461235	.	engineered_region	3442	3482	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461235	.	promoter	2688	2887	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1461223
BBa_K1461235	.	non_covalent_binding_site	2776	2813	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1461235	.	non_covalent_binding_site	2853	2887	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1461235	.	promoter	2824	2829	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1461235	.	engineered_region	2688	2887	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1461235	.	promoter	2848	2853	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1461235	.	start_codon	2860	2860	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1461235	.	CDS	2688	2775	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1461235	.	engineered_region	1	2679	.	+	0	ID=BBa_K1461233;Name=BBa_K1461233
BBa_K1461235	.	engineered_region	1855	2679	.	+	0	ID=BBa_K1461221;Name=BBa_K1461221;Parent=BBa_K1461233
BBa_K1461235	.	promoter	1855	1934	.	+	0	ID=BBa_K1461001;Name=BBa_K1461001;Parent=BBa_K1461221
BBa_K1461235	.	CDS	1961	2542	.	+	0	ID=BBa_K1461004;Name=BBa_K1461004;Parent=BBa_K1461221
BBa_K1461235	.	terminator	2551	2679	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461221
BBa_K1461235	.	terminator	2551	2630	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461235	.	engineered_region	2551	2630	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461235	.	stem_loop	2562	2605	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461235	.	terminator	2639	2679	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461235	.	stop_codon	2672	2672	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461235	.	polyA_site	2666	2679	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461235	.	stem_loop	2646	2665	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461235	.	engineered_region	2639	2679	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461235	.	ribosome_entry_site	1943	1954	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461221
BBa_K1461235	.	conserved_region	1947	1950	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461235	.	engineered_region	1	1846	.	+	0	ID=BBa_K1461225;Name=BBa_K1461225;Parent=BBa_K1461233
BBa_K1461235	.	engineered_region	1	875	.	+	0	ID=BBa_K1461209;Name=BBa_K1461209;Parent=BBa_K1461225
BBa_K1461235	.	engineered_region	139	875	.	+	0	ID=BBa_K1461201;Name=BBa_K1461201;Parent=BBa_K1461209
BBa_K1461235	.	terminator	747	875	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461201
BBa_K1461235	.	terminator	747	826	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461235	.	engineered_region	747	826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461235	.	stem_loop	758	801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461235	.	terminator	835	875	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461235	.	stop_codon	868	868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461235	.	polyA_site	862	875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461235	.	stem_loop	842	861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461235	.	engineered_region	835	875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461235	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461201
BBa_K1461235	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461235	.	CDS	157	738	.	+	0	ID=BBa_K1461004;Name=BBa_K1461004;Parent=BBa_K1461201
BBa_K1461235	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K1461209
BBa_K1461235	.	engineered_region	884	1846	.	+	0	ID=BBa_K1461219;Name=BBa_K1461219;Parent=BBa_K1461225
BBa_K1461235	.	CDS	990	1709	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K1461219
BBa_K1461235	.	CDS	990	1709	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1461235	.	promoter	884	963	.	+	0	ID=BBa_K1461001;Name=BBa_K1461001;Parent=BBa_K1461219
BBa_K1461235	.	terminator	1718	1846	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461219
BBa_K1461235	.	terminator	1718	1797	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461235	.	engineered_region	1718	1797	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461235	.	stem_loop	1729	1772	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461235	.	terminator	1806	1846	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461235	.	stop_codon	1839	1839	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461235	.	polyA_site	1833	1846	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461235	.	stem_loop	1813	1832	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461235	.	engineered_region	1806	1846	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461235	.	ribosome_entry_site	972	983	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461219
BBa_K1461235	.	conserved_region	976	979	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1461235
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacc
cgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaa
gctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggc
gctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctg
gatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgc
cggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagat
tagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcg
gatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagaggcgcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagc
tgcgttcatcagcgatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccc
aattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatac
ggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtt
atggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtta
tgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacc
cttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaact
ataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacat
tgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagac
aaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttg
taacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagaggcgcggataaaaatttcatttgcccgcgacggattccccgcc
catctatcgttgaacccatcagctgcgttcatcagcgatactagagaaagaggagaaatactagatgaacgaaaccgat
ccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgc
gcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcat
ttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgc
tatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccgg
cgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcg
cgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcg
ctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggcc
gtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaccccgg
aacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggc
actgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttat
caggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagcc
gctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggc
actgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagc
attaacgcggaagaagcggaacagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata