LOCUS BBa_K1461236 3767 bp DNA linear 05-Nov-2024 DEFINITION resettable counter device using ecf11 (w/o crRBS, taRNA) ACCESSION BBa_K1461236 VERSION BBa_K1461236.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 157..765 /label=BBa_K1461005 stem_loop 785..828 /label=stem_loop misc_feature 774..853 /label=BBa_B0010 terminator 774..853 /label=BBa_B0010 stem_loop 869..888 /label=T7 TE misc_feature 895..895 /label=stop polyA_site 889..902 /label=polya misc_feature 862..902 /label=BBa_B0012 terminator 862..902 /label=BBa_B0012 terminator 774..902 /label=BBa_B0015 misc_feature 139..902 /label=BBa_K1461202 misc_feature 1..902 /label=BBa_K1461211 promoter 911..990 /label=BBa_K1461002 misc_feature 1003..1006 /label=conserved RBS 999..1010 /label=BBa_B0034 CDS 1017..1736 /label=GFP protein CDS 1017..1736 /label=BBa_E0040 stem_loop 1756..1799 /label=stem_loop misc_feature 1745..1824 /label=BBa_B0010 terminator 1745..1824 /label=BBa_B0010 stem_loop 1840..1859 /label=T7 TE misc_feature 1866..1866 /label=stop polyA_site 1860..1873 /label=polya misc_feature 1833..1873 /label=BBa_B0012 terminator 1833..1873 /label=BBa_B0012 terminator 1745..1873 /label=BBa_B0015 misc_feature 911..1873 /label=BBa_K1461220 misc_feature 1..1873 /label=BBa_K1461226 promoter 1882..1961 /label=BBa_K1461002 misc_feature 1974..1977 /label=conserved RBS 1970..1981 /label=BBa_B0034 CDS 1988..2596 /label=BBa_K1461005 stem_loop 2616..2659 /label=stem_loop misc_feature 2605..2684 /label=BBa_B0010 terminator 2605..2684 /label=BBa_B0010 stem_loop 2700..2719 /label=T7 TE misc_feature 2726..2726 /label=stop polyA_site 2720..2733 /label=polya misc_feature 2693..2733 /label=BBa_B0012 terminator 2693..2733 /label=BBa_B0012 terminator 2605..2733 /label=BBa_B0015 misc_feature 1882..2733 /label=BBa_K1461222 misc_feature 1..2733 /label=BBa_K1461234 CDS 2742..2829 /label=end of LacI coding region (inactive) misc_feature 2742..2941 /label=BBa_R0010 misc_feature 2830..2867 /label=CAP binding site promoter 2878..2883 /label=-35 promoter 2902..2907 /label=-10 misc_feature 2907..2941 /label=LacI binding site misc_feature 2914..2914 /label=start promoter 2742..2941 /label=BBa_R0010 misc_feature 2954..2957 /label=conserved RBS 2950..2961 /label=BBa_B0034 CDS 2968..3630 /label=BBa_K1461007 stem_loop 3650..3693 /label=stem_loop misc_feature 3639..3718 /label=BBa_B0010 terminator 3639..3718 /label=BBa_B0010 stem_loop 3734..3753 /label=T7 TE misc_feature 3760..3760 /label=stop polyA_site 3754..3767 /label=polya misc_feature 3727..3767 /label=BBa_B0012 terminator 3727..3767 /label=BBa_B0012 terminator 3639..3767 /label=BBa_B0015 misc_feature 2950..3767 /label=BBa_K1461207 misc_feature 2742..3767 /label=BBa_K1461224 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatgc gcattaccgc gagcctgcgc 181 accttttgcc atctgagcac cccgcatagc gatagcacca ccagccgcct gtggattgat 241 gaagtgaccg cggtggcgcg ccagcgcgat cgcgatagct ttatgcgcat ttatgatcat 301 tttgcgccgc gcctgctgcg ctatctgacc ggcctgaacg tgccggaagg ccaggcggaa 361 gaactggtgc aggaagtgct gctgaaactg tggcataaag cggaaagctt tgatccgagc 421 aaagcgagcc tgggcacctg gctgtttcgc attgcgcgca acctgtatat tgatagcgtg 481 cgcaaagatc gcggctgggt gcaggtgcag aacagcctgg aacagctgga acgcctggaa 541 gcgccggtgg atcgcaccct ggattatagc cagcgccagg aacagcagct gaacagcgcg 601 attcagaacc tgccgaccga tcaggcgaaa gtgctgcgca tgagctattt tgaagcgctg 661 agccatcgcg aaattagcga acgcctggat atgccgctgg gcaccgtgaa aagctgcctg 721 cgcctggcgt ttcagaaact gcgcagccgc attgaagaaa gctaatacta gagccaggca 781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 901 tatactagag gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg 961 taacacctct gcatcgcgaa ccaaaaccag tactagagaa agaggagaaa tactagatgc 1021 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg 1081 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 1141 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca 1201 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg 1261 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag 1321 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 1381 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg 1441 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 1501 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt 1561 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 1621 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 1681 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact 1741 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1801 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1861 ttctgcgttt atatactaga ggcctccaca ccgctcgtca catcctgtga tccactcttc 1921 atcccgctac gtaacacctc tgcatcgcga accaaaacca gtactagaga aagaggagaa 1981 atactagatg cgcattaccg cgagcctgcg caccttttgc catctgagca ccccgcatag 2041 cgatagcacc accagccgcc tgtggattga tgaagtgacc gcggtggcgc gccagcgcga 2101 tcgcgatagc tttatgcgca tttatgatca ttttgcgccg cgcctgctgc gctatctgac 2161 cggcctgaac gtgccggaag gccaggcgga agaactggtg caggaagtgc tgctgaaact 2221 gtggcataaa gcggaaagct ttgatccgag caaagcgagc ctgggcacct ggctgtttcg 2281 cattgcgcgc aacctgtata ttgatagcgt gcgcaaagat cgcggctggg tgcaggtgca 2341 gaacagcctg gaacagctgg aacgcctgga agcgccggtg gatcgcaccc tggattatag 2401 ccagcgccag gaacagcagc tgaacagcgc gattcagaac ctgccgaccg atcaggcgaa 2461 agtgctgcgc atgagctatt ttgaagcgct gagccatcgc gaaattagcg aacgcctgga 2521 tatgccgctg ggcaccgtga aaagctgcct gcgcctggcg tttcagaaac tgcgcagccg 2581 cattgaagaa agctaatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 2641 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 2701 gctcaccttc gggtgggcct ttctgcgttt atatactaga gcaatacgca aaccgcctct 2761 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 2821 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 2881 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 2941 atactagaga aagaggagaa atactagatg ggcccgctgc atcatccgga tgatgcgacc 3001 ctgattagct atgcggcagg cgcactgagc caggtgattg cggtggtgac cgcagcgcat 3061 ctggaacgtt gcgcagaatg ccgtgcacgt ctgcgtcagg cagaacagat tggtggcgtg 3121 ctgatgcagc agagcattag ccgtgtggtg ccgctgaaaa gccgtatggc gatgctggca 3181 cgcctggatg aacaggaaac cagcgtggat agcgtgatgc atgcgatgcc ggcagcgaac 3241 catgatccgg atctgctgcc gcattgcatg catgcgcatt ttggccgcca tctgagcacc 3301 ctgaaatgga aaaccctgat tccgggcgtg cagcgcgtga gcgcacaggg cattgaacag 3361 ggcaacctga tgctgctgaa aattgcacca ggcgtgagca tgccggtgca tagccatgaa 3421 agcggtgaaa tgaccatggt gctgaaaggc gcgtatcatg atgtgcaggg tgaatttggc 3481 ctgaacgatg tggcagatct ggatagccat attcagcatc agccgattgc gtatccggat 3541 cgcgaatgca tttgcgtgct ggcggcagaa agcaaactgc gttttcatgg ctggatggca 3601 cgtatgatgc agccgttttt tggcatttaa tactagagcc aggcatcaaa taaaacgaaa 3661 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3721 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //