BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K1461234 1 BBa_K1461234 ecf11 promoter mesurement system (positive feedback) 2014-09-28T11:00:00Z 2015-05-08T01:10:34Z Assembly. This part has ecf11 positive feedback system. Arabinose-induction causes ecf11 expression, and once ecf11 expressed, then the positive feedback system is activated and generate ecf11 continually. ecf11 remains in the cell and continue to activate the expression of GFP under ecf11 promoter. false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2401835 1 BBa_K1461222 component2401823 1 BBa_K1461226 annotation2401823 1 BBa_K1461226 range2401823 1 1 1873 annotation2401835 1 BBa_K1461222 range2401835 1 1882 2733 BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_K1461236 1 BBa_K1461236 resettable counter device using ecf11 (w/o crRBS, taRNA) 2014-09-28T11:00:00Z 2015-05-08T01:10:34Z Assembly This part is a basic device in our &#963;-Re Counter project without riboregulator system. Once arabinoce is added, the cell generates ecf11, and ecf11 positive feedback is activated. The chassis continue to generate ecf11, which activates the expression of GFP. false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2401934 1 BBa_K1461234 component2401953 1 BBa_K1461224 annotation2401953 1 BBa_K1461224 range2401953 1 2742 3767 annotation2401934 1 BBa_K1461234 range2401934 1 1 2733 BBa_K1461007 1 BBa_K1461007 anti ecf11 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Syringae. This part is from Pseudomonas Syringae RpoE. ecf11 is a sigma factor which activates ecf11 promoter (BBa_K1461002) false false _1840_ 0 18036 9 It's complicated true None. false Takefumi Yoshikawa BBa_K1461224 1 BBa_K1461224 anti ecf11 generator (pLac) 2014-09-26T11:00:00Z 2015-05-08T01:10:33Z Assembly. This generator codes anti ecf11 (BBa_K1461007), which prevents ecf11 (BBa_K1461005) from activating the expression of ecf11 promoter (BBa_K1461002). This part works as a reset factor. false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2387514 1 BBa_R0010 component2387531 1 BBa_K1461207 annotation2387531 1 BBa_K1461207 range2387531 1 209 1026 annotation2387514 1 BBa_R0010 range2387514 1 1 200 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1461002 1 BBa_K1461002 ecf11 promoter 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Syringae. This part is from Pseudomonas Syringae RpoE. ecf20 (BBa_K1461005) activates the expression of the downstream genes of this promoter. false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K1461226 1 BBa_K1461226 ecf11 promoter measurement system 2014-09-28T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part can be used to characterize the combination of ecf11 (BBa_K1461005) and ecf11 promoter (BBa_K1461002), using GFP as reporter gene. false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2401454 1 BBa_K1461211 component2401467 1 BBa_K1461220 annotation2401454 1 BBa_K1461211 range2401454 1 1 902 annotation2401467 1 BBa_K1461220 range2401467 1 911 1873 BBa_K1461005 1 BBa_K1461005 ecf11 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Syringae. This part is from Pseudomonas Syringae RpoE. ecf11 is a sigma factor which activates ecf11 promoter (BBa_K1461002) false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_K1461220 1 BBa_K1461220 ecf11-induced GFP generator 2014-09-26T11:00:00Z 2015-05-08T01:10:33Z Assembly. This generator codes GFP (BBa_E0040), induced by ecf11 (BBa_K1461005). false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2387397 1 BBa_B0034 component2387399 1 BBa_E0040 component2387395 1 BBa_K1461002 component2387406 1 BBa_B0015 annotation2387399 1 BBa_E0040 range2387399 1 107 826 annotation2387397 1 BBa_B0034 range2387397 1 89 100 annotation2387395 1 BBa_K1461002 range2387395 1 1 80 annotation2387406 1 BBa_B0015 range2387406 1 835 963 BBa_K1461222 1 BBa_K1461222 ecf11 generator (positive feedback) 2014-09-26T11:00:00Z 2015-05-08T01:10:33Z Assembly. This generator codes ecf11 (BBa_K1461005), under ecf11 promoter (BBa_K1461002). This positive feedback in our &#963;-Re Counter project enables the chassis, E.coli, to remain memory. false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2387422 1 BBa_B0034 component2387420 1 BBa_K1461002 component2387423 1 BBa_K1461005 component2387430 1 BBa_B0015 annotation2387423 1 BBa_K1461005 range2387423 1 107 715 annotation2387420 1 BBa_K1461002 range2387420 1 1 80 annotation2387430 1 BBa_B0015 range2387430 1 724 852 annotation2387422 1 BBa_B0034 range2387422 1 89 100 BBa_K1461202 1 BBa_K1461202 ecf11 (+RBS, +d.term) 2014-09-24T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of RBS (BBa_B0034), ecf11 (BBa_K1461005),and double terminator (BBa_B0015). false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2386280 1 BBa_K1461005 component2386279 1 BBa_B0034 component2386287 1 BBa_B0015 annotation2386279 1 BBa_B0034 range2386279 1 1 12 annotation2386287 1 BBa_B0015 range2386287 1 636 764 annotation2386280 1 BBa_K1461005 range2386280 1 19 627 BBa_K1461211 1 BBa_K1461211 arabinose-induced ecf11 generator 2014-09-25T11:00:00Z 2015-05-08T01:10:33Z Assembly. This generator codes ecf11, a sigma factor, constitutively. false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2401083 1 BBa_K1461202 component2401072 1 BBa_I13453 annotation2401072 1 BBa_I13453 range2401072 1 1 130 annotation2401083 1 BBa_K1461202 range2401083 1 139 902 BBa_K1461207 1 BBa_K1461207 anti ecf11 (+RBS, +d.term) 2014-09-25T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of RBS (BBa_B0034), anti ecf20 (BBa_K1461007), and double terminator (BBa_B0015). false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2386625 1 BBa_K1461007 component2386632 1 BBa_B0015 component2386624 1 BBa_B0034 annotation2386625 1 BBa_K1461007 range2386625 1 19 681 annotation2386632 1 BBa_B0015 range2386632 1 690 818 annotation2386624 1 BBa_B0034 range2386624 1 1 12 BBa_K1461005_sequence 1 atgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaa BBa_K1461211_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1461007_sequence 1 atgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1461234_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461202_sequence 1 aaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461236_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1461002_sequence 1 gcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccag BBa_K1461207_sequence 1 aaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461220_sequence 1 gcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461224_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461226_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1461222_sequence 1 gcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z