LOCUS       BBa_K1461237            1873 bp    DNA     linear       22-Sep-2024
DEFINITION  sigma factors cross-talk assay 1
ACCESSION   BBa_K1461237
VERSION     BBa_K1461237.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..765
                     /label=BBa_K1461005
     stem_loop       785..828
                     /label=stem_loop
     misc_feature    774..853
                     /label=BBa_B0010
     terminator      774..853
                     /label=BBa_B0010
     stem_loop       869..888
                     /label=T7 TE
     misc_feature    895..895
                     /label=stop
     polyA_site      889..902
                     /label=polya
     misc_feature    862..902
                     /label=BBa_B0012
     terminator      862..902
                     /label=BBa_B0012
     terminator      774..902
                     /label=BBa_B0015
     misc_feature    139..902
                     /label=BBa_K1461202
     misc_feature    1..902
                     /label=BBa_K1461211
     promoter        911..990
                     /label=BBa_K1461001
     misc_feature    1003..1006
                     /label=conserved
     RBS             999..1010
                     /label=BBa_B0034
     CDS             1017..1736
                     /label=GFP protein
     CDS             1017..1736
                     /label=BBa_E0040
     stem_loop       1756..1799
                     /label=stem_loop
     misc_feature    1745..1824
                     /label=BBa_B0010
     terminator      1745..1824
                     /label=BBa_B0010
     stem_loop       1840..1859
                     /label=T7 TE
     misc_feature    1866..1866
                     /label=stop
     polyA_site      1860..1873
                     /label=polya
     misc_feature    1833..1873
                     /label=BBa_B0012
     terminator      1833..1873
                     /label=BBa_B0012
     terminator      1745..1873
                     /label=BBa_B0015
     misc_feature    911..1873
                     /label=BBa_K1461219
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgc gcattaccgc gagcctgcgc
      181 accttttgcc atctgagcac cccgcatagc gatagcacca ccagccgcct gtggattgat
      241 gaagtgaccg cggtggcgcg ccagcgcgat cgcgatagct ttatgcgcat ttatgatcat
      301 tttgcgccgc gcctgctgcg ctatctgacc ggcctgaacg tgccggaagg ccaggcggaa
      361 gaactggtgc aggaagtgct gctgaaactg tggcataaag cggaaagctt tgatccgagc
      421 aaagcgagcc tgggcacctg gctgtttcgc attgcgcgca acctgtatat tgatagcgtg
      481 cgcaaagatc gcggctgggt gcaggtgcag aacagcctgg aacagctgga acgcctggaa
      541 gcgccggtgg atcgcaccct ggattatagc cagcgccagg aacagcagct gaacagcgcg
      601 attcagaacc tgccgaccga tcaggcgaaa gtgctgcgca tgagctattt tgaagcgctg
      661 agccatcgcg aaattagcga acgcctggat atgccgctgg gcaccgtgaa aagctgcctg
      721 cgcctggcgt ttcagaaact gcgcagccgc attgaagaaa gctaatacta gagccaggca
      781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      901 tatactagag gcgcggataa aaatttcatt tgcccgcgac ggattccccg cccatctatc
      961 gttgaaccca tcagctgcgt tcatcagcga tactagagaa agaggagaaa tactagatgc
     1021 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
     1081 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
     1141 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
     1201 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
     1261 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1321 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     1381 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1441 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1501 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     1561 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1621 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     1681 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact
     1741 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1801 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1861 ttctgcgttt ata
//