LOCUS BBa_K1461239 3821 bp DNA linear 23-Dec-2024 DEFINITION σ-Re Counter (double, using ecf20) ACCESSION BBa_K1461239 VERSION BBa_K1461239.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 139..209 /label=BBa_K1461003 stem_loop 229..272 /label=stem_loop misc_feature 218..297 /label=BBa_B0010 terminator 218..297 /label=BBa_B0010 stem_loop 313..332 /label=T7 TE misc_feature 339..339 /label=stop polyA_site 333..346 /label=polya misc_feature 306..346 /label=BBa_B0012 terminator 306..346 /label=BBa_B0012 terminator 218..346 /label=BBa_B0015 misc_feature 139..346 /label=BBa_K1461200 misc_feature 1..346 /label=BBa_K1461208 promoter 355..389 /label=BBa_J23101 RBS 398..449 /label=rbs RBS 398..449 /label=BBa_K1461000 CDS 456..1037 /label=BBa_K1461004 stem_loop 1057..1100 /label=stem_loop misc_feature 1046..1125 /label=BBa_B0010 terminator 1046..1125 /label=BBa_B0010 stem_loop 1141..1160 /label=T7 TE misc_feature 1167..1167 /label=stop polyA_site 1161..1174 /label=polya misc_feature 1134..1174 /label=BBa_B0012 terminator 1134..1174 /label=BBa_B0012 terminator 1046..1174 /label=BBa_B0015 misc_feature 398..1174 /label=BBa_K1461203 misc_feature 355..1174 /label=BBa_K1461214 promoter 1183..1262 /label=BBa_K1461001 misc_feature 1275..1278 /label=conserved RBS 1271..1282 /label=BBa_B0034 CDS 1289..1870 /label=BBa_K1461004 stem_loop 1890..1933 /label=stem_loop misc_feature 1879..1958 /label=BBa_B0010 terminator 1879..1958 /label=BBa_B0010 stem_loop 1974..1993 /label=T7 TE misc_feature 2000..2000 /label=stop polyA_site 1994..2007 /label=polya misc_feature 1967..2007 /label=BBa_B0012 terminator 1967..2007 /label=BBa_B0012 terminator 1879..2007 /label=BBa_B0015 misc_feature 1183..2007 /label=BBa_K1461221 promoter 2016..2095 /label=BBa_K1461001 RBS 2104..2155 /label=rbs RBS 2104..2155 /label=BBa_K1461000 CDS 2162..2881 /label=GFP protein CDS 2162..2881 /label=BBa_E0040 stem_loop 2901..2944 /label=stem_loop misc_feature 2890..2969 /label=BBa_B0010 terminator 2890..2969 /label=BBa_B0010 stem_loop 2985..3004 /label=T7 TE misc_feature 3011..3011 /label=stop polyA_site 3005..3018 /label=polya misc_feature 2978..3018 /label=BBa_B0012 terminator 2978..3018 /label=BBa_B0012 terminator 2890..3018 /label=BBa_B0015 misc_feature 2104..3018 /label=BBa_K1461205 CDS 3027..3114 /label=end of LacI coding region (inactive) misc_feature 3027..3226 /label=BBa_R0010 misc_feature 3115..3152 /label=CAP binding site promoter 3163..3168 /label=-35 promoter 3187..3192 /label=-10 misc_feature 3192..3226 /label=LacI binding site misc_feature 3199..3199 /label=start promoter 3027..3226 /label=BBa_R0010 misc_feature 3239..3242 /label=conserved RBS 3235..3246 /label=BBa_B0034 CDS 3253..3684 /label=BBa_K1461006 stem_loop 3704..3747 /label=stem_loop misc_feature 3693..3772 /label=BBa_B0010 terminator 3693..3772 /label=BBa_B0010 stem_loop 3788..3807 /label=T7 TE misc_feature 3814..3814 /label=stop polyA_site 3808..3821 /label=polya misc_feature 3781..3821 /label=BBa_B0012 terminator 3781..3821 /label=BBa_B0012 terminator 3693..3821 /label=BBa_B0015 misc_feature 3235..3821 /label=BBa_K1461206 misc_feature 3027..3821 /label=BBa_K1461223 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagac ccaaatccag gaggtgattg gtagtggtgg ttaatgaaaa 181 ttaacttact actaccatat atctccagat actagagcca ggcatcaaat aaaacgaaag 241 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 301 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtttaca 361 gctagctcag tcctaggtat tatgctagct actagaggaa ctctaccatt cacctcttgg 421 atttgggtat taaagaggag aaaggtacct actagatgaa cgaaaccgat ccggatctgg 481 aactgctgaa acgtattggc aacaacgatg cacaggcggt gaaagaaatg gtgacccgca 541 aactgccgcg cctgctggcg ctggcgagcc gtctgctggg cgatgcggat gaagcgcgcg 601 atattgcgca ggaaagcttt ctgcgcattt ggaaacaggc agcgagctgg cgcagcgaac 661 aggcgcgctt tgatacctgg ctgcatcgcg tggcgctgaa cctgtgctat gatcgcctgc 721 gccgtcgtaa agaacatgtg ccggtggata gcgaacatgc gtgcgaagcg ctggataccc 781 gcccggcgcc ggatgaacag ctggaagcga gcgcgcagag ccgtcgcatg gcgcaggcac 841 tggatcagct gccggatcgt cagcgcgaag cgattgtgct gcaatattat caggaactga 901 gcaacaccga agcagcggca ctgatgcaga ttagcgtgga agcgctggaa agcctgctga 961 gccgcgcacg tcgcaacctg cgcagccatc tggcggaagc gccgggtgcg gatctgagcg 1021 gccgtcgcaa accgtaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1081 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1141 ggctcacctt cgggtgggcc tttctgcgtt tatatactag aggcgcggat aaaaatttca 1201 tttgcccgcg acggattccc cgcccatcta tcgttgaacc catcagctgc gttcatcagc 1261 gatactagag aaagaggaga aatactagat gaacgaaacc gatccggatc tggaactgct 1321 gaaacgtatt ggcaacaacg atgcacaggc ggtgaaagaa atggtgaccc gcaaactgcc 1381 gcgcctgctg gcgctggcga gccgtctgct gggcgatgcg gatgaagcgc gcgatattgc 1441 gcaggaaagc tttctgcgca tttggaaaca ggcagcgagc tggcgcagcg aacaggcgcg 1501 ctttgatacc tggctgcatc gcgtggcgct gaacctgtgc tatgatcgcc tgcgccgtcg 1561 taaagaacat gtgccggtgg atagcgaaca tgcgtgcgaa gcgctggata cccgcccggc 1621 gccggatgaa cagctggaag cgagcgcgca gagccgtcgc atggcgcagg cactggatca 1681 gctgccggat cgtcagcgcg aagcgattgt gctgcaatat tatcaggaac tgagcaacac 1741 cgaagcagcg gcactgatgc agattagcgt ggaagcgctg gaaagcctgc tgagccgcgc 1801 acgtcgcaac ctgcgcagcc atctggcgga agcgccgggt gcggatctga gcggccgtcg 1861 caaaccgtaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1921 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1981 cttcgggtgg gcctttctgc gtttatatac tagaggcgcg gataaaaatt tcatttgccc 2041 gcgacggatt ccccgcccat ctatcgttga acccatcagc tgcgttcatc agcgatacta 2101 gaggaactct accattcacc tcttggattt gggtattaaa gaggagaaag gtacctacta 2161 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg 2221 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg 2281 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact 2341 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca 2401 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt 2461 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt 2521 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa 2581 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg 2641 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga 2701 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta 2761 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct 2821 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata 2881 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2941 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3001 ggcctttctg cgtttatata ctagagcaat acgcaaaccg cctctccccg cgcgttggcc 3061 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 3121 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 3181 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag 3241 gagaaatact agatgacccc ggaacgtttt gtgcatctgg cggatgcgta tggtgcagat 3301 ctgcaacgtt ggccgagcgc agaacgtgca gcagcacagg cactgctgga ttgcggcgat 3361 gcgcaggcag tggcagcgct gcgtcaggca cattggctgg atagccagct ggatcgttat 3421 caggtgccgg caccgagccc ggcactggca cagcgtatta ttgcggcagc acaacagccg 3481 ggtgcaccgt tttggagccg ctatgcaggt tggctggcaa gcctgggttg ggtgggtgtg 3541 ggcctgacag gcgtggcagc gggtatgctg gcggtggcac tgagcctgcc attaagcacc 3601 agcgcagaag cactgccaag cgtgtttgat cagagcgatg cagaatttgt gctgagcatt 3661 aacgcggaag aagcggaaca gtaatactag agccaggcat caaataaaac gaaaggctca 3721 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3781 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //