##gff-version 3
##sequence-region BBa_K1461239 1 3821
BBa_K1461239	.	engineered_region	1	346	.	+	0	ID=BBa_K1461208;Name=BBa_K1461208
BBa_K1461239	.	engineered_region	139	346	.	+	0	ID=BBa_K1461200;Name=BBa_K1461200;Parent=BBa_K1461208
BBa_K1461239	.	mature_transcript_region	139	209	.	+	0	ID=BBa_K1461003;Name=BBa_K1461003;Parent=BBa_K1461200
BBa_K1461239	.	terminator	218	346	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461200
BBa_K1461239	.	terminator	306	346	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461239	.	polyA_site	333	346	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461239	.	engineered_region	306	346	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461239	.	stem_loop	313	332	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461239	.	stop_codon	339	339	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461239	.	terminator	218	297	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461239	.	stem_loop	229	272	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461239	.	engineered_region	218	297	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461239	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K1461208
BBa_K1461239	.	engineered_region	3027	3821	.	+	0	ID=BBa_K1461223;Name=BBa_K1461223
BBa_K1461239	.	engineered_region	3235	3821	.	+	0	ID=BBa_K1461206;Name=BBa_K1461206;Parent=BBa_K1461223
BBa_K1461239	.	CDS	3253	3684	.	+	0	ID=BBa_K1461006;Name=BBa_K1461006;Parent=BBa_K1461206
BBa_K1461239	.	ribosome_entry_site	3235	3246	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461206
BBa_K1461239	.	conserved_region	3239	3242	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461239	.	terminator	3693	3821	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461206
BBa_K1461239	.	terminator	3781	3821	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461239	.	polyA_site	3808	3821	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461239	.	engineered_region	3781	3821	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461239	.	stem_loop	3788	3807	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461239	.	stop_codon	3814	3814	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461239	.	terminator	3693	3772	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461239	.	stem_loop	3704	3747	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461239	.	engineered_region	3693	3772	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461239	.	promoter	3027	3226	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1461223
BBa_K1461239	.	start_codon	3199	3199	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1461239	.	CDS	3027	3114	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1461239	.	promoter	3187	3192	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1461239	.	non_covalent_binding_site	3192	3226	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1461239	.	non_covalent_binding_site	3115	3152	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1461239	.	engineered_region	3027	3226	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1461239	.	promoter	3163	3168	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1461239	.	promoter	2016	2095	.	+	0	ID=BBa_K1461001;Name=BBa_K1461001
BBa_K1461239	.	engineered_region	355	1174	.	+	0	ID=BBa_K1461214;Name=BBa_K1461214
BBa_K1461239	.	promoter	355	389	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1461214
BBa_K1461239	.	engineered_region	398	1174	.	+	0	ID=BBa_K1461203;Name=BBa_K1461203;Parent=BBa_K1461214
BBa_K1461239	.	terminator	1046	1174	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461203
BBa_K1461239	.	terminator	1134	1174	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461239	.	polyA_site	1161	1174	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461239	.	engineered_region	1134	1174	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461239	.	stem_loop	1141	1160	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461239	.	stop_codon	1167	1167	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461239	.	terminator	1046	1125	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461239	.	stem_loop	1057	1100	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461239	.	engineered_region	1046	1125	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461239	.	CDS	456	1037	.	+	0	ID=BBa_K1461004;Name=BBa_K1461004;Parent=BBa_K1461203
BBa_K1461239	.	ribosome_entry_site	398	449	.	+	0	ID=BBa_K1461000;Name=BBa_K1461000;Parent=BBa_K1461203
BBa_K1461239	.	ribosome_entry_site	398	449	.	+	0	ID=annotation2384852;Name=rbs;Parent=BBa_K1461000
BBa_K1461239	.	engineered_region	2104	3018	.	+	0	ID=BBa_K1461205;Name=BBa_K1461205
BBa_K1461239	.	CDS	2162	2881	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K1461205
BBa_K1461239	.	CDS	2162	2881	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1461239	.	terminator	2890	3018	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461205
BBa_K1461239	.	terminator	2978	3018	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461239	.	polyA_site	3005	3018	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461239	.	engineered_region	2978	3018	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461239	.	stem_loop	2985	3004	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461239	.	stop_codon	3011	3011	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461239	.	terminator	2890	2969	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461239	.	stem_loop	2901	2944	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461239	.	engineered_region	2890	2969	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461239	.	ribosome_entry_site	2104	2155	.	+	0	ID=BBa_K1461000;Name=BBa_K1461000;Parent=BBa_K1461205
BBa_K1461239	.	ribosome_entry_site	2104	2155	.	+	0	ID=annotation2384852;Name=rbs;Parent=BBa_K1461000
BBa_K1461239	.	engineered_region	1183	2007	.	+	0	ID=BBa_K1461221;Name=BBa_K1461221
BBa_K1461239	.	promoter	1183	1262	.	+	0	ID=BBa_K1461001;Name=BBa_K1461001;Parent=BBa_K1461221
BBa_K1461239	.	terminator	1879	2007	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461221
BBa_K1461239	.	terminator	1967	2007	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461239	.	polyA_site	1994	2007	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461239	.	engineered_region	1967	2007	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461239	.	stem_loop	1974	1993	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461239	.	stop_codon	2000	2000	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461239	.	terminator	1879	1958	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461239	.	stem_loop	1890	1933	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461239	.	engineered_region	1879	1958	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461239	.	ribosome_entry_site	1271	1282	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461221
BBa_K1461239	.	conserved_region	1275	1278	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461239	.	CDS	1289	1870	.	+	0	ID=BBa_K1461004;Name=BBa_K1461004;Parent=BBa_K1461221
>BBa_K1461239
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagacccaaatccaggaggtgat
tggtagtggtggttaatgaaaattaacttactactaccatatatctccagatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactag
aggaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatgaacgaaaccgatccgg
atctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcct
gctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttgg
aaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatg
atcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgcc
ggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaa
gcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctgg
aaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcg
caaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcg
cggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagctgcgttcatcagcgata
ctagagaaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatg
cacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcgga
tgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgc
tttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtgg
atagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcg
catggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaac
accgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgca
gccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagaggcgcggataaaaatttcatttgcccgcgacggattcccc
gcccatctatcgttgaacccatcagctgcgttcatcagcgatactagaggaactctaccattcacctcttggatttggg
tattaaagaggagaaaggtacctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggca
cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccag
gctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaaga
ggagaaatactagatgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagc
gcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggc
tggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaaca
gccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtg
gcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcaga
gcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata