##gff-version 3
##sequence-region BBa_K1461240 1 4106
BBa_K1461240	.	promoter	2070	2149	.	+	0	ID=BBa_K1461002;Name=BBa_K1461002
BBa_K1461240	.	engineered_region	1	346	.	+	0	ID=BBa_K1461208;Name=BBa_K1461208
BBa_K1461240	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K1461208
BBa_K1461240	.	engineered_region	139	346	.	+	0	ID=BBa_K1461200;Name=BBa_K1461200;Parent=BBa_K1461208
BBa_K1461240	.	terminator	218	346	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461200
BBa_K1461240	.	terminator	218	297	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461240	.	engineered_region	218	297	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461240	.	stem_loop	229	272	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461240	.	terminator	306	346	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461240	.	stem_loop	313	332	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461240	.	stop_codon	339	339	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461240	.	engineered_region	306	346	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461240	.	polyA_site	333	346	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461240	.	mature_transcript_region	139	209	.	+	0	ID=BBa_K1461003;Name=BBa_K1461003;Parent=BBa_K1461200
BBa_K1461240	.	engineered_region	355	1201	.	+	0	ID=BBa_K1461216;Name=BBa_K1461216
BBa_K1461240	.	engineered_region	398	1201	.	+	0	ID=BBa_K1461204;Name=BBa_K1461204;Parent=BBa_K1461216
BBa_K1461240	.	terminator	1073	1201	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461204
BBa_K1461240	.	terminator	1073	1152	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461240	.	engineered_region	1073	1152	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461240	.	stem_loop	1084	1127	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461240	.	terminator	1161	1201	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461240	.	stem_loop	1168	1187	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461240	.	stop_codon	1194	1194	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461240	.	engineered_region	1161	1201	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461240	.	polyA_site	1188	1201	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461240	.	CDS	456	1064	.	+	0	ID=BBa_K1461005;Name=BBa_K1461005;Parent=BBa_K1461204
BBa_K1461240	.	ribosome_entry_site	398	449	.	+	0	ID=BBa_K1461000;Name=BBa_K1461000;Parent=BBa_K1461204
BBa_K1461240	.	ribosome_entry_site	398	449	.	+	0	ID=annotation2384852;Name=rbs;Parent=BBa_K1461000
BBa_K1461240	.	promoter	355	389	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1461216
BBa_K1461240	.	engineered_region	3081	4106	.	+	0	ID=BBa_K1461224;Name=BBa_K1461224
BBa_K1461240	.	engineered_region	3289	4106	.	+	0	ID=BBa_K1461207;Name=BBa_K1461207;Parent=BBa_K1461224
BBa_K1461240	.	ribosome_entry_site	3289	3300	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461207
BBa_K1461240	.	conserved_region	3293	3296	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461240	.	CDS	3307	3969	.	+	0	ID=BBa_K1461007;Name=BBa_K1461007;Parent=BBa_K1461207
BBa_K1461240	.	terminator	3978	4106	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461207
BBa_K1461240	.	terminator	3978	4057	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461240	.	engineered_region	3978	4057	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461240	.	stem_loop	3989	4032	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461240	.	terminator	4066	4106	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461240	.	stem_loop	4073	4092	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461240	.	stop_codon	4099	4099	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461240	.	engineered_region	4066	4106	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461240	.	polyA_site	4093	4106	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461240	.	promoter	3081	3280	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1461224
BBa_K1461240	.	promoter	3241	3246	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1461240	.	promoter	3217	3222	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1461240	.	start_codon	3253	3253	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1461240	.	non_covalent_binding_site	3246	3280	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1461240	.	CDS	3081	3168	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1461240	.	non_covalent_binding_site	3169	3206	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1461240	.	engineered_region	3081	3280	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1461240	.	engineered_region	1210	2061	.	+	0	ID=BBa_K1461222;Name=BBa_K1461222
BBa_K1461240	.	terminator	1933	2061	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461222
BBa_K1461240	.	terminator	1933	2012	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461240	.	engineered_region	1933	2012	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461240	.	stem_loop	1944	1987	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461240	.	terminator	2021	2061	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461240	.	stem_loop	2028	2047	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461240	.	stop_codon	2054	2054	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461240	.	engineered_region	2021	2061	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461240	.	polyA_site	2048	2061	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461240	.	promoter	1210	1289	.	+	0	ID=BBa_K1461002;Name=BBa_K1461002;Parent=BBa_K1461222
BBa_K1461240	.	CDS	1316	1924	.	+	0	ID=BBa_K1461005;Name=BBa_K1461005;Parent=BBa_K1461222
BBa_K1461240	.	ribosome_entry_site	1298	1309	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461222
BBa_K1461240	.	conserved_region	1302	1305	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461240	.	engineered_region	2158	3072	.	+	0	ID=BBa_K1461205;Name=BBa_K1461205
BBa_K1461240	.	CDS	2216	2935	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K1461205
BBa_K1461240	.	CDS	2216	2935	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1461240	.	terminator	2944	3072	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461205
BBa_K1461240	.	terminator	2944	3023	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461240	.	engineered_region	2944	3023	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461240	.	stem_loop	2955	2998	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461240	.	terminator	3032	3072	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461240	.	stem_loop	3039	3058	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461240	.	stop_codon	3065	3065	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461240	.	engineered_region	3032	3072	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461240	.	polyA_site	3059	3072	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461240	.	ribosome_entry_site	2158	2209	.	+	0	ID=BBa_K1461000;Name=BBa_K1461000;Parent=BBa_K1461205
BBa_K1461240	.	ribosome_entry_site	2158	2209	.	+	0	ID=annotation2384852;Name=rbs;Parent=BBa_K1461000
>BBa_K1461240
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagacccaaatccaggaggtgat
tggtagtggtggttaatgaaaattaacttactactaccatatatctccagatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactag
aggaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatgcgcattaccgcgagcc
tgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggt
ggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggc
ctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttg
atccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcg
cggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagc
cagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctatt
ttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggc
gtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaaca
cctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcacct
tttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgcca
gcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtg
ccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagca
aagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggt
gcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccag
gaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgc
tgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaa
actgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcat
cgcgaaccaaaaccagtactagaggaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagc
aatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg
ggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctc
gtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctg
catcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgc
atctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcat
tagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtg
atgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctga
gcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgct
gctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcg
tatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgt
atccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgca
gccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata