BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1461240 1 BBa_K1461240 &#963;-Re Counter (double, using ecf11) 2014-09-28T11:00:00Z 2015-05-08T01:10:34Z Assembly. This part is resettable double counter using ecf11, a sigma factor. For detail information, see our wiki. false false _1840_ 0 18036 9 Not in stock false None. false Takefumi Yoshikawa component2402084 1 BBa_K1461208 component2402097 1 BBa_K1461216 component2402141 1 BBa_K1461224 component2402109 1 BBa_K1461222 component2402122 1 BBa_K1461205 component2402110 1 BBa_K1461002 annotation2402097 1 BBa_K1461216 range2402097 1 355 1201 annotation2402110 1 BBa_K1461002 range2402110 1 2070 2149 annotation2402109 1 BBa_K1461222 range2402109 1 1210 2061 annotation2402122 1 BBa_K1461205 range2402122 1 2158 3072 annotation2402141 1 BBa_K1461224 range2402141 1 3081 4106 annotation2402084 1 BBa_K1461208 range2402084 1 1 346 BBa_K1461204 1 BBa_K1461204 cr-ecf11 (+RBS, +d.term) 2014-09-24T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of crRBS (BBa_K1461000), ecf11 (BBa_K1461005), double terminator (BBa_B0015). With promoter, mRNA which codes ecf11 will be transcripted, but not usually translated. taRNA (BBa_K1461003) activates the translation. false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2386560 1 BBa_B0015 component2386553 1 BBa_K1461005 component2386552 1 BBa_K1461000 annotation2386552 1 BBa_K1461000 range2386552 1 1 52 annotation2386553 1 BBa_K1461005 range2386553 1 59 667 annotation2386560 1 BBa_B0015 range2386560 1 676 804 BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_K1461205 1 BBa_K1461205 cr-GFP (+RBS, +d.term) 2014-09-24T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of crRBS (BBa_K1461000), GFP (BBa_E0040), double terminator (BBa_B0015). With promoter, mRNA which codes GFP will be transcripted, but not usually translated. taRNA (BBa_K1461003) activates the translation. false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2386564 1 BBa_E0040 component2386571 1 BBa_B0015 component2386562 1 BBa_K1461000 annotation2386562 1 BBa_K1461000 range2386562 1 1 52 annotation2386564 1 BBa_E0040 range2386564 1 59 778 annotation2386571 1 BBa_B0015 range2386571 1 787 915 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1461224 1 BBa_K1461224 anti ecf11 generator (pLac) 2014-09-26T11:00:00Z 2015-05-08T01:10:33Z Assembly. This generator codes anti ecf11 (BBa_K1461007), which prevents ecf11 (BBa_K1461005) from activating the expression of ecf11 promoter (BBa_K1461002). This part works as a reset factor. false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2387531 1 BBa_K1461207 component2387514 1 BBa_R0010 annotation2387531 1 BBa_K1461207 range2387531 1 209 1026 annotation2387514 1 BBa_R0010 range2387514 1 1 200 BBa_K1461007 1 BBa_K1461007 anti ecf11 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Syringae. This part is from Pseudomonas Syringae RpoE. ecf11 is a sigma factor which activates ecf11 promoter (BBa_K1461002) false false _1840_ 0 18036 9 It's complicated true None. false Takefumi Yoshikawa BBa_K1461208 1 BBa_K1461208 taRNA (arabinose-induced) (+d.term) 2014-09-25T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is arabinose-induced taRNA generator. This part works as a trigger of &#963;-ReCounter. false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2386642 1 BBa_K1461200 component2386633 1 BBa_I13453 annotation2386633 1 BBa_I13453 range2386633 1 1 130 annotation2386642 1 BBa_K1461200 range2386642 1 139 346 BBa_K1461207 1 BBa_K1461207 anti ecf11 (+RBS, +d.term) 2014-09-25T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of RBS (BBa_B0034), anti ecf20 (BBa_K1461007), and double terminator (BBa_B0015). false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2386632 1 BBa_B0015 component2386624 1 BBa_B0034 component2386625 1 BBa_K1461007 annotation2386624 1 BBa_B0034 range2386624 1 1 12 annotation2386625 1 BBa_K1461007 range2386625 1 19 681 annotation2386632 1 BBa_B0015 range2386632 1 690 818 BBa_K1461002 1 BBa_K1461002 ecf11 promoter 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Syringae. This part is from Pseudomonas Syringae RpoE. ecf20 (BBa_K1461005) activates the expression of the downstream genes of this promoter. false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_K1461222 1 BBa_K1461222 ecf11 generator (positive feedback) 2014-09-26T11:00:00Z 2015-05-08T01:10:33Z Assembly. This generator codes ecf11 (BBa_K1461005), under ecf11 promoter (BBa_K1461002). This positive feedback in our &#963;-Re Counter project enables the chassis, E.coli, to remain memory. false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2387420 1 BBa_K1461002 component2387422 1 BBa_B0034 component2387423 1 BBa_K1461005 component2387430 1 BBa_B0015 annotation2387423 1 BBa_K1461005 range2387423 1 107 715 annotation2387422 1 BBa_B0034 range2387422 1 89 100 annotation2387430 1 BBa_B0015 range2387430 1 724 852 annotation2387420 1 BBa_K1461002 range2387420 1 1 80 BBa_K1461003 1 BBa_K1461003 taRNA 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Artifixcial. This part hybridizes to crRBS (BBa_K1461000) and promotes the translation of the downstream genes of crRBS. false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_K1461005 1 BBa_K1461005 ecf11 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Syringae. This part is from Pseudomonas Syringae RpoE. ecf11 is a sigma factor which activates ecf11 promoter (BBa_K1461002) false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1461216 1 BBa_K1461216 constitutive cr-ecf11 generator 2014-09-25T11:00:00Z 2015-05-08T01:10:33Z Assembly. The transcription of this generator is induced by arabinose, and the translation of ecf20 is activated by taRNA (BBa_K1461003). false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2401278 1 BBa_K1461204 component2401267 1 BBa_J23101 annotation2401267 1 BBa_J23101 range2401267 1 1 35 annotation2401278 1 BBa_K1461204 range2401278 1 44 847 BBa_K1461200 1 BBa_K1461200 taRNA (+d.term) 2014-09-24T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of taRNA (BBa_K1461003) and double terminator (BBa_B0015). false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2386259 1 BBa_K1461003 component2386266 1 BBa_B0015 annotation2386259 1 BBa_K1461003 range2386259 1 1 71 annotation2386266 1 BBa_B0015 range2386266 1 80 208 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1461000 1 BBa_K1461000 crRBS 2014-09-21T11:00:00Z 2015-05-08T01:10:33Z Artificial. This part is ribosome-binding site (RBS) with cis-repressor (cr) sequence. The cis-repressor sequence is placed between the transcription start site and the RBS, and its complementarity with the RBS causes a stem-loop structure to form upon transcription. This secondary structure prevents binding of the 30S ribosomal subunit to the RBS, which inhibits translation. false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa annotation2384852 1 rbs range2384852 1 1 52 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K1461005_sequence 1 atgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaa BBa_K1461000_sequence 1 gaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacc BBa_K1461003_sequence 1 acccaaatccaggaggtgattggtagtggtggttaatgaaaattaacttactactaccatatatctccaga BBa_K1461200_sequence 1 acccaaatccaggaggtgattggtagtggtggttaatgaaaattaacttactactaccatatatctccagatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1461007_sequence 1 atgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaa BBa_K1461240_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagacccaaatccaggaggtgattggtagtggtggttaatgaaaattaacttactactaccatatatctccagatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagaggaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagaggaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K1461205_sequence 1 gaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461216_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagaggaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1461002_sequence 1 gcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccag BBa_K1461207_sequence 1 aaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461208_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagacccaaatccaggaggtgattggtagtggtggttaatgaaaattaacttactactaccatatatctccagatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461224_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcccgctgcatcatccggatgatgcgaccctgattagctatgcggcaggcgcactgagccaggtgattgcggtggtgaccgcagcgcatctggaacgttgcgcagaatgccgtgcacgtctgcgtcaggcagaacagattggtggcgtgctgatgcagcagagcattagccgtgtggtgccgctgaaaagccgtatggcgatgctggcacgcctggatgaacaggaaaccagcgtggatagcgtgatgcatgcgatgccggcagcgaaccatgatccggatctgctgccgcattgcatgcatgcgcattttggccgccatctgagcaccctgaaatggaaaaccctgattccgggcgtgcagcgcgtgagcgcacagggcattgaacagggcaacctgatgctgctgaaaattgcaccaggcgtgagcatgccggtgcatagccatgaaagcggtgaaatgaccatggtgctgaaaggcgcgtatcatgatgtgcagggtgaatttggcctgaacgatgtggcagatctggatagccatattcagcatcagccgattgcgtatccggatcgcgaatgcatttgcgtgctggcggcagaaagcaaactgcgttttcatggctggatggcacgtatgatgcagccgttttttggcatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1461204_sequence 1 gaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461222_sequence 1 gcctccacaccgctcgtcacatcctgtgatccactcttcatcccgctacgtaacacctctgcatcgcgaaccaaaaccagtactagagaaagaggagaaatactagatgcgcattaccgcgagcctgcgcaccttttgccatctgagcaccccgcatagcgatagcaccaccagccgcctgtggattgatgaagtgaccgcggtggcgcgccagcgcgatcgcgatagctttatgcgcatttatgatcattttgcgccgcgcctgctgcgctatctgaccggcctgaacgtgccggaaggccaggcggaagaactggtgcaggaagtgctgctgaaactgtggcataaagcggaaagctttgatccgagcaaagcgagcctgggcacctggctgtttcgcattgcgcgcaacctgtatattgatagcgtgcgcaaagatcgcggctgggtgcaggtgcagaacagcctggaacagctggaacgcctggaagcgccggtggatcgcaccctggattatagccagcgccaggaacagcagctgaacagcgcgattcagaacctgccgaccgatcaggcgaaagtgctgcgcatgagctattttgaagcgctgagccatcgcgaaattagcgaacgcctggatatgccgctgggcaccgtgaaaagctgcctgcgcctggcgtttcagaaactgcgcagccgcattgaagaaagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z