This part is resettable double counter using ecf11, a sigma factor. For detail information, see our wiki.
This part is from Pseudomonas Syringae RpoE. ecf20 (BBa_K1461005) activates the expression of the downstream genes of this promoter.
This part is a composite part of crRBS (BBa_K1461000), GFP (BBa_E0040), double terminator (BBa_B0015). With promoter, mRNA which codes GFP will be transcripted, but not usually translated. taRNA (BBa_K1461003) activates the translation.
This part is arabinose-induced taRNA generator. This part works as a trigger of σ-ReCounter.
The transcription of this generator is induced by arabinose, and the translation of ecf20 is activated by taRNA (BBa_K1461003).
This generator codes ecf11 (BBa_K1461005), under ecf11 promoter (BBa_K1461002). This positive feedback in our σ-Re Counter project enables the chassis, E.coli, to remain memory.
This generator codes anti ecf11 (BBa_K1461007), which prevents ecf11 (BBa_K1461005) from activating the expression of ecf11 promoter (BBa_K1461002). This part works as a reset factor.
This part is ribosome-binding site (RBS) with cis-repressor (cr) sequence. The cis-repressor sequence is placed between the transcription start site and the RBS, and its complementarity with the RBS causes a stem-loop structure to form upon transcription. This secondary structure prevents binding of the 30S ribosomal subunit to the RBS, which inhibits translation.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
Double terminator consisting of BBa_B0010 and BBa_B0012
This part is from Pseudomonas Syringae RpoE. ecf11 is a sigma factor which activates ecf11 promoter (BBa_K1461002)
This part is a composite part of taRNA (BBa_K1461003) and double terminator (BBa_B0015).
This part is a composite part of crRBS (BBa_K1461000), ecf11 (BBa_K1461005), double terminator (BBa_B0015). With promoter, mRNA which codes ecf11 will be transcripted, but not usually translated. taRNA (BBa_K1461003) activates the translation.
This part is a composite part of RBS (BBa_B0034), anti ecf20 (BBa_K1461007), and double terminator (BBa_B0015).
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
This part hybridizes to crRBS (BBa_K1461000) and promotes the translation of the downstream genes of crRBS.
Conversion of the iGEM parts registry to SBOL2.1