LOCUS BBa_K1461241 6615 bp DNA linear 23-Dec-2024 DEFINITION σ-Re Counter (triple) ACCESSION BBa_K1461241 VERSION BBa_K1461241.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 139..209 /label=BBa_K1461003 stem_loop 229..272 /label=stem_loop misc_feature 218..297 /label=BBa_B0010 terminator 218..297 /label=BBa_B0010 stem_loop 313..332 /label=T7 TE misc_feature 339..339 /label=stop polyA_site 333..346 /label=polya misc_feature 306..346 /label=BBa_B0012 terminator 306..346 /label=BBa_B0012 terminator 218..346 /label=BBa_B0015 misc_feature 139..346 /label=BBa_K1461200 misc_feature 1..346 /label=BBa_K1461208 promoter 355..389 /label=BBa_J23101 RBS 398..449 /label=rbs RBS 398..449 /label=BBa_K1461000 CDS 456..1037 /label=BBa_K1461004 stem_loop 1057..1100 /label=stem_loop misc_feature 1046..1125 /label=BBa_B0010 terminator 1046..1125 /label=BBa_B0010 stem_loop 1141..1160 /label=T7 TE misc_feature 1167..1167 /label=stop polyA_site 1161..1174 /label=polya misc_feature 1134..1174 /label=BBa_B0012 terminator 1134..1174 /label=BBa_B0012 terminator 1046..1174 /label=BBa_B0015 misc_feature 398..1174 /label=BBa_K1461203 misc_feature 355..1174 /label=BBa_K1461214 promoter 1183..1262 /label=BBa_K1461001 misc_feature 1275..1278 /label=conserved RBS 1271..1282 /label=BBa_B0034 CDS 1289..1870 /label=BBa_K1461004 stem_loop 1890..1933 /label=stem_loop misc_feature 1879..1958 /label=BBa_B0010 terminator 1879..1958 /label=BBa_B0010 stem_loop 1974..1993 /label=T7 TE misc_feature 2000..2000 /label=stop polyA_site 1994..2007 /label=polya misc_feature 1967..2007 /label=BBa_B0012 terminator 1967..2007 /label=BBa_B0012 terminator 1879..2007 /label=BBa_B0015 misc_feature 1183..2007 /label=BBa_K1461221 promoter 2016..2095 /label=BBa_K1461001 RBS 2104..2155 /label=rbs RBS 2104..2155 /label=BBa_K1461000 CDS 2162..2770 /label=BBa_K1461005 stem_loop 2790..2833 /label=stem_loop misc_feature 2779..2858 /label=BBa_B0010 terminator 2779..2858 /label=BBa_B0010 stem_loop 2874..2893 /label=T7 TE misc_feature 2900..2900 /label=stop polyA_site 2894..2907 /label=polya misc_feature 2867..2907 /label=BBa_B0012 terminator 2867..2907 /label=BBa_B0012 terminator 2779..2907 /label=BBa_B0015 misc_feature 2104..2907 /label=BBa_K1461204 promoter 2916..2995 /label=BBa_K1461002 misc_feature 3008..3011 /label=conserved RBS 3004..3015 /label=BBa_B0034 CDS 3022..3630 /label=BBa_K1461005 stem_loop 3650..3693 /label=stem_loop misc_feature 3639..3718 /label=BBa_B0010 terminator 3639..3718 /label=BBa_B0010 stem_loop 3734..3753 /label=T7 TE misc_feature 3760..3760 /label=stop polyA_site 3754..3767 /label=polya misc_feature 3727..3767 /label=BBa_B0012 terminator 3727..3767 /label=BBa_B0012 terminator 3639..3767 /label=BBa_B0015 misc_feature 2916..3767 /label=BBa_K1461222 promoter 3776..3855 /label=BBa_K1461002 RBS 3864..3915 /label=rbs RBS 3864..3915 /label=BBa_K1461000 CDS 3922..4641 /label=GFP protein CDS 3922..4641 /label=BBa_E0040 stem_loop 4661..4704 /label=stem_loop misc_feature 4650..4729 /label=BBa_B0010 terminator 4650..4729 /label=BBa_B0010 stem_loop 4745..4764 /label=T7 TE misc_feature 4771..4771 /label=stop polyA_site 4765..4778 /label=polya misc_feature 4738..4778 /label=BBa_B0012 terminator 4738..4778 /label=BBa_B0012 terminator 4650..4778 /label=BBa_B0015 misc_feature 3864..4778 /label=BBa_K1461205 CDS 4787..4874 /label=end of LacI coding region (inactive) misc_feature 4787..4986 /label=BBa_R0010 misc_feature 4875..4912 /label=CAP binding site promoter 4923..4928 /label=-35 promoter 4947..4952 /label=-10 misc_feature 4952..4986 /label=LacI binding site misc_feature 4959..4959 /label=start promoter 4787..4986 /label=BBa_R0010 misc_feature 4999..5002 /label=conserved RBS 4995..5006 /label=BBa_B0034 CDS 5013..5444 /label=BBa_K1461006 stem_loop 5464..5507 /label=stem_loop misc_feature 5453..5532 /label=BBa_B0010 terminator 5453..5532 /label=BBa_B0010 stem_loop 5548..5567 /label=T7 TE misc_feature 5574..5574 /label=stop polyA_site 5568..5581 /label=polya misc_feature 5541..5581 /label=BBa_B0012 terminator 5541..5581 /label=BBa_B0012 terminator 5453..5581 /label=BBa_B0015 misc_feature 4995..5581 /label=BBa_K1461206 misc_feature 4787..5581 /label=BBa_K1461223 CDS 5590..5677 /label=end of LacI coding region (inactive) misc_feature 5590..5789 /label=BBa_R0010 misc_feature 5678..5715 /label=CAP binding site promoter 5726..5731 /label=-35 promoter 5750..5755 /label=-10 misc_feature 5755..5789 /label=LacI binding site misc_feature 5762..5762 /label=start promoter 5590..5789 /label=BBa_R0010 misc_feature 5802..5805 /label=conserved RBS 5798..5809 /label=BBa_B0034 CDS 5816..6478 /label=BBa_K1461007 stem_loop 6498..6541 /label=stem_loop misc_feature 6487..6566 /label=BBa_B0010 terminator 6487..6566 /label=BBa_B0010 stem_loop 6582..6601 /label=T7 TE misc_feature 6608..6608 /label=stop polyA_site 6602..6615 /label=polya misc_feature 6575..6615 /label=BBa_B0012 terminator 6575..6615 /label=BBa_B0012 terminator 6487..6615 /label=BBa_B0015 misc_feature 5798..6615 /label=BBa_K1461207 misc_feature 5590..6615 /label=BBa_K1461224 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagac ccaaatccag gaggtgattg gtagtggtgg ttaatgaaaa 181 ttaacttact actaccatat atctccagat actagagcca ggcatcaaat aaaacgaaag 241 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 301 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtttaca 361 gctagctcag tcctaggtat tatgctagct actagaggaa ctctaccatt cacctcttgg 421 atttgggtat taaagaggag aaaggtacct actagatgaa cgaaaccgat ccggatctgg 481 aactgctgaa acgtattggc aacaacgatg cacaggcggt gaaagaaatg gtgacccgca 541 aactgccgcg cctgctggcg ctggcgagcc gtctgctggg cgatgcggat gaagcgcgcg 601 atattgcgca ggaaagcttt ctgcgcattt ggaaacaggc agcgagctgg cgcagcgaac 661 aggcgcgctt tgatacctgg ctgcatcgcg tggcgctgaa cctgtgctat gatcgcctgc 721 gccgtcgtaa agaacatgtg ccggtggata gcgaacatgc gtgcgaagcg ctggataccc 781 gcccggcgcc ggatgaacag ctggaagcga gcgcgcagag ccgtcgcatg gcgcaggcac 841 tggatcagct gccggatcgt cagcgcgaag cgattgtgct gcaatattat caggaactga 901 gcaacaccga agcagcggca ctgatgcaga ttagcgtgga agcgctggaa agcctgctga 961 gccgcgcacg tcgcaacctg cgcagccatc tggcggaagc gccgggtgcg gatctgagcg 1021 gccgtcgcaa accgtaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1081 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1141 ggctcacctt cgggtgggcc tttctgcgtt tatatactag aggcgcggat aaaaatttca 1201 tttgcccgcg acggattccc cgcccatcta tcgttgaacc catcagctgc gttcatcagc 1261 gatactagag aaagaggaga aatactagat gaacgaaacc gatccggatc tggaactgct 1321 gaaacgtatt ggcaacaacg atgcacaggc ggtgaaagaa atggtgaccc gcaaactgcc 1381 gcgcctgctg gcgctggcga gccgtctgct gggcgatgcg gatgaagcgc gcgatattgc 1441 gcaggaaagc tttctgcgca tttggaaaca ggcagcgagc tggcgcagcg aacaggcgcg 1501 ctttgatacc tggctgcatc gcgtggcgct gaacctgtgc tatgatcgcc tgcgccgtcg 1561 taaagaacat gtgccggtgg atagcgaaca tgcgtgcgaa gcgctggata cccgcccggc 1621 gccggatgaa cagctggaag cgagcgcgca gagccgtcgc atggcgcagg cactggatca 1681 gctgccggat cgtcagcgcg aagcgattgt gctgcaatat tatcaggaac tgagcaacac 1741 cgaagcagcg gcactgatgc agattagcgt ggaagcgctg gaaagcctgc tgagccgcgc 1801 acgtcgcaac ctgcgcagcc atctggcgga agcgccgggt gcggatctga gcggccgtcg 1861 caaaccgtaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1921 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1981 cttcgggtgg gcctttctgc gtttatatac tagaggcgcg gataaaaatt tcatttgccc 2041 gcgacggatt ccccgcccat ctatcgttga acccatcagc tgcgttcatc agcgatacta 2101 gaggaactct accattcacc tcttggattt gggtattaaa gaggagaaag gtacctacta 2161 gatgcgcatt accgcgagcc tgcgcacctt ttgccatctg agcaccccgc atagcgatag 2221 caccaccagc cgcctgtgga ttgatgaagt gaccgcggtg gcgcgccagc gcgatcgcga 2281 tagctttatg cgcatttatg atcattttgc gccgcgcctg ctgcgctatc tgaccggcct 2341 gaacgtgccg gaaggccagg cggaagaact ggtgcaggaa gtgctgctga aactgtggca 2401 taaagcggaa agctttgatc cgagcaaagc gagcctgggc acctggctgt ttcgcattgc 2461 gcgcaacctg tatattgata gcgtgcgcaa agatcgcggc tgggtgcagg tgcagaacag 2521 cctggaacag ctggaacgcc tggaagcgcc ggtggatcgc accctggatt atagccagcg 2581 ccaggaacag cagctgaaca gcgcgattca gaacctgccg accgatcagg cgaaagtgct 2641 gcgcatgagc tattttgaag cgctgagcca tcgcgaaatt agcgaacgcc tggatatgcc 2701 gctgggcacc gtgaaaagct gcctgcgcct ggcgtttcag aaactgcgca gccgcattga 2761 agaaagctaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 2821 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 2881 cttcgggtgg gcctttctgc gtttatatac tagaggcctc cacaccgctc gtcacatcct 2941 gtgatccact cttcatcccg ctacgtaaca cctctgcatc gcgaaccaaa accagtacta 3001 gagaaagagg agaaatacta gatgcgcatt accgcgagcc tgcgcacctt ttgccatctg 3061 agcaccccgc atagcgatag caccaccagc cgcctgtgga ttgatgaagt gaccgcggtg 3121 gcgcgccagc gcgatcgcga tagctttatg cgcatttatg atcattttgc gccgcgcctg 3181 ctgcgctatc tgaccggcct gaacgtgccg gaaggccagg cggaagaact ggtgcaggaa 3241 gtgctgctga aactgtggca taaagcggaa agctttgatc cgagcaaagc gagcctgggc 3301 acctggctgt ttcgcattgc gcgcaacctg tatattgata gcgtgcgcaa agatcgcggc 3361 tgggtgcagg tgcagaacag cctggaacag ctggaacgcc tggaagcgcc ggtggatcgc 3421 accctggatt atagccagcg ccaggaacag cagctgaaca gcgcgattca gaacctgccg 3481 accgatcagg cgaaagtgct gcgcatgagc tattttgaag cgctgagcca tcgcgaaatt 3541 agcgaacgcc tggatatgcc gctgggcacc gtgaaaagct gcctgcgcct ggcgtttcag 3601 aaactgcgca gccgcattga agaaagctaa tactagagcc aggcatcaaa taaaacgaaa 3661 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3721 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagaggcctc 3781 cacaccgctc gtcacatcct gtgatccact cttcatcccg ctacgtaaca cctctgcatc 3841 gcgaaccaaa accagtacta gaggaactct accattcacc tcttggattt gggtattaaa 3901 gaggagaaag gtacctacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca 3961 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt 4021 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta 4081 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga 4141 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta 4201 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag 4261 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat 4321 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg 4381 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat 4441 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc 4501 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa 4561 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg 4621 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc 4681 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 4741 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat acgcaaaccg 4801 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 4861 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 4921 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 4981 cacacatact agagaaagag gagaaatact agatgacccc ggaacgtttt gtgcatctgg 5041 cggatgcgta tggtgcagat ctgcaacgtt ggccgagcgc agaacgtgca gcagcacagg 5101 cactgctgga ttgcggcgat gcgcaggcag tggcagcgct gcgtcaggca cattggctgg 5161 atagccagct ggatcgttat caggtgccgg caccgagccc ggcactggca cagcgtatta 5221 ttgcggcagc acaacagccg ggtgcaccgt tttggagccg ctatgcaggt tggctggcaa 5281 gcctgggttg ggtgggtgtg ggcctgacag gcgtggcagc gggtatgctg gcggtggcac 5341 tgagcctgcc attaagcacc agcgcagaag cactgccaag cgtgtttgat cagagcgatg 5401 cagaatttgt gctgagcatt aacgcggaag aagcggaaca gtaatactag agccaggcat 5461 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 5521 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 5581 atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 5641 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 5701 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 5761 gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat actagatggg 5821 cccgctgcat catccggatg atgcgaccct gattagctat gcggcaggcg cactgagcca 5881 ggtgattgcg gtggtgaccg cagcgcatct ggaacgttgc gcagaatgcc gtgcacgtct 5941 gcgtcaggca gaacagattg gtggcgtgct gatgcagcag agcattagcc gtgtggtgcc 6001 gctgaaaagc cgtatggcga tgctggcacg cctggatgaa caggaaacca gcgtggatag 6061 cgtgatgcat gcgatgccgg cagcgaacca tgatccggat ctgctgccgc attgcatgca 6121 tgcgcatttt ggccgccatc tgagcaccct gaaatggaaa accctgattc cgggcgtgca 6181 gcgcgtgagc gcacagggca ttgaacaggg caacctgatg ctgctgaaaa ttgcaccagg 6241 cgtgagcatg ccggtgcata gccatgaaag cggtgaaatg accatggtgc tgaaaggcgc 6301 gtatcatgat gtgcagggtg aatttggcct gaacgatgtg gcagatctgg atagccatat 6361 tcagcatcag ccgattgcgt atccggatcg cgaatgcatt tgcgtgctgg cggcagaaag 6421 caaactgcgt tttcatggct ggatggcacg tatgatgcag ccgttttttg gcatttaata 6481 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 6541 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 6601 ctttctgcgt ttata //