LOCUS       BBa_K1461302            1001 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS-GFP-4 miR 142 3p non-binding site-d.term
ACCESSION   BBa_K1461302
VERSION     BBa_K1461302.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     misc_feature    747..771
                     /label=binding
     misc_feature    747..771
                     /label=BBa_K1461100
     misc_feature    780..802
                     /label=misc
     misc_feature    780..802
                     /label=BBa_K1461102
     misc_feature    811..833
                     /label=misc
     misc_feature    811..833
                     /label=BBa_K1461102
     misc_feature    842..864
                     /label=misc
     misc_feature    842..864
                     /label=BBa_K1461102
     stem_loop       884..927
                     /label=stem_loop
     misc_feature    873..952
                     /label=BBa_B0010
     terminator      873..952
                     /label=BBa_B0010
     stem_loop       968..987
                     /label=T7 TE
     misc_feature    994..994
                     /label=stop
     polyA_site      988..1001
                     /label=polya
     misc_feature    961..1001
                     /label=BBa_B0012
     terminator      961..1001
                     /label=BBa_B0012
     terminator      873..1001
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagaggtgt agtgtttcct actttatgga ttactagagg
      781 agtagtgctt tctactttat gttactagag gagtagtgct ttctacttta tgttactaga
      841 ggagtagtgc tttctacttt atgttactag agccaggcat caaataaaac gaaaggctca
      901 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      961 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//