LOCUS       BBa_K1461305            1003 bp    DNA     linear       22-Nov-2024
DEFINITION  RBS-GFP-2miR 142 3p non-bindng sites-2miR 142 5p non-bindng 
            sites-d.term
ACCESSION   BBa_K1461305
VERSION     BBa_K1461305.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     misc_feature    747..769
                     /label=misc
     misc_feature    747..769
                     /label=BBa_K1461102
     misc_feature    778..800
                     /label=misc
     misc_feature    778..800
                     /label=BBa_K1461102
     misc_feature    809..831
                     /label=misc
     misc_feature    809..833
                     /label=BBa_K1461103
     misc_feature    842..864
                     /label=misc
     misc_feature    842..866
                     /label=BBa_K1461103
     stem_loop       886..929
                     /label=stem_loop
     misc_feature    875..954
                     /label=BBa_B0010
     terminator      875..954
                     /label=BBa_B0010
     stem_loop       970..989
                     /label=T7 TE
     misc_feature    996..996
                     /label=stop
     polyA_site      990..1003
                     /label=polya
     misc_feature    963..1003
                     /label=BBa_B0012
     terminator      963..1003
                     /label=BBa_B0012
     terminator      875..1003
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagaggagt agtgctttct actttatgtt actagaggag
      781 tagtgctttc tactttatgt tactagaggt ccataaagta ggaaacacta cattactaga
      841 ggtccataaa gtaggaaaca ctacattact agagccaggc atcaaataaa acgaaaggct
      901 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      961 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//