LOCUS       BBa_K1461308            1589 bp    DNA     linear       22-Nov-2024
DEFINITION  pCMV-RBS-GFP-4 miR 142 3p non-binding sites-d.term
ACCESSION   BBa_K1461308
VERSION     BBa_K1461308.1  
FEATURES             Location/Qualifiers
     promoter        1..580
                     /label=CMV
     promoter        1..580
                     /label=BBa_K747096
     misc_feature    593..596
                     /label=conserved
     RBS             589..600
                     /label=BBa_B0034
     CDS             607..1326
                     /label=GFP protein
     CDS             607..1326
                     /label=BBa_E0040
     misc_feature    1335..1359
                     /label=binding
     misc_feature    1335..1359
                     /label=BBa_K1461100
     misc_feature    1368..1390
                     /label=misc
     misc_feature    1368..1390
                     /label=BBa_K1461102
     misc_feature    1399..1421
                     /label=misc
     misc_feature    1399..1421
                     /label=BBa_K1461102
     misc_feature    1430..1452
                     /label=misc
     misc_feature    1430..1452
                     /label=BBa_K1461102
     stem_loop       1472..1515
                     /label=stem_loop
     misc_feature    1461..1540
                     /label=BBa_B0010
     terminator      1461..1540
                     /label=BBa_B0010
     stem_loop       1556..1575
                     /label=T7 TE
     misc_feature    1582..1582
                     /label=stop
     polyA_site      1576..1589
                     /label=polya
     misc_feature    1549..1589
                     /label=BBa_B0012
     terminator      1549..1589
                     /label=BBa_B0012
     terminator      1461..1589
                     /label=BBa_B0015
     misc_feature    589..1589
                     /label=BBa_K1461302
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagagaa agaggagaaa
      601 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      661 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      721 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      781 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      841 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      901 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      961 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     1021 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
     1081 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     1141 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     1201 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     1261 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     1321 taataatact agaggtgtag tgtttcctac tttatggatt actagaggag tagtgctttc
     1381 tactttatgt tactagagga gtagtgcttt ctactttatg ttactagagg agtagtgctt
     1441 tctactttat gttactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1501 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1561 accttcgggt gggcctttct gcgtttata
//