BBa_K1461308
type: ComponentDefinition
persistent identity: BBa K1461308
display id: BBa_K1461308
version: 1
was generated by: iGEM to SBOL conversion
description: pCMV-RBS-GFP-4 miR 142 3p non-binding sites-d.term
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:34Z
owned by: james
owned by: myers
mutable description: This is a GFP generator. This generator is used for characterization of translation inhibition by miR-142-3p in HL60 as a control. This contains pCMV, RBS, GFP, 4 miR-142-3p non-binding sites and double terminator.
discontinued: false
dominant: false
group u list: _1840_
m user id: 0
owner id: 16849
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Shunsuke Sumi
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1461302
component: BBa_K747096
sequence annotation: BBa_K747096
sequence annotation: BBa_K1461302
sequence: https://synbiohub.org/public/igem/BBa_K1461308_sequence/1

BBa_K1461302
type: Component
persistent identity: component2389969
display id: component2389969
version: 1
top level: BBa_K1461308
definition: BBa_K1461302
access: public

BBa_K747096
type: Component
persistent identity: component2389949
display id: component2389949
version: 1
top level: BBa_K1461308
definition: BBa_K747096
access: public

BBa_K747096
type: SequenceAnnotation
persistent identity: annotation2389949
display id: annotation2389949
version: 1
top level: BBa_K1461308
location: https://synbiohub.org/public/igem/BBa_K1461308/annotation2389949/range2389949/1
component: BBa_K747096

https://synbiohub.org/public/igem/BBa_K1461308/annotation2389949/range2389949/1
type: Range
persistent identity: range2389949
display id: range2389949
version: 1
top level: BBa_K1461308
direction: direction/reverse
start: 1
end: 580
orientation: inline

BBa_K1461302
type: SequenceAnnotation
persistent identity: annotation2389969
display id: annotation2389969
version: 1
top level: BBa_K1461308
location: https://synbiohub.org/public/igem/BBa_K1461308/annotation2389969/range2389969/1
component: BBa_K1461302

https://synbiohub.org/public/igem/BBa_K1461308/annotation2389969/range2389969/1
type: Range
persistent identity: range2389969
display id: range2389969
version: 1
top level: BBa_K1461308
direction: direction/reverse
start: 589
end: 1589
orientation: inline

BBa_K747096
type: ComponentDefinition
persistent identity: BBa K747096
display id: BBa_K747096
version: 1
was generated by: iGEM to SBOL conversion
description: CMV-promoter
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:11Z
mutable provenance: not sure
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: follows
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: c
star: false
creator: Lucas Schneider
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CMV
sequence: https://synbiohub.org/public/igem/BBa_K747096_sequence/1

CMV
type: SequenceAnnotation
persistent identity: annotation2199147
display id: annotation2199147
version: 1
top level: BBa_K747096
location: https://synbiohub.org/public/igem/BBa_K747096/annotation2199147/range2199147/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K747096/annotation2199147/range2199147/1
type: Range
persistent identity: range2199147
display id: range2199147
version: 1
top level: BBa_K747096
direction: direction/forward
start: 1
end: 580
orientation: inline

BBa_K1461302
type: ComponentDefinition
persistent identity: BBa K1461302
display id: BBa_K1461302
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-GFP-4 miR 142 3p non-binding site-d.term
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:34Z
owned by: james
owned by: myers
mutable description: This is used as control for characterization of translation inhibition by miR-142-5p in HL60. This contains RBS, GFP, 4 miR-142-5p non-binding sites and double terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 16849
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Shunsuke Sumi
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1461102
component: BBa_K1461100
component: BBa_E0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_K1461100
sequence annotation: BBa_K1461102
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1461302_sequence/1

BBa_B0015
type: Component
persistent identity: component2389789
display id: component2389789
version: 1
top level: BBa_K1461302
definition: BBa_B0015
access: public

BBa_K1461102
type: Component
persistent identity: component2389782
display id: component2389782
version: 1
top level: BBa_K1461302
definition: BBa_K1461102
access: public

BBa_K1461102
type: Component
persistent identity: component2389780
display id: component2389780
version: 1
top level: BBa_K1461302
definition: BBa_K1461102
access: public

BBa_K1461102
type: Component
persistent identity: component2389778
display id: component2389778
version: 1
top level: BBa_K1461302
definition: BBa_K1461102
access: public

BBa_K1461100
type: Component
persistent identity: component2389776
display id: component2389776
version: 1
top level: BBa_K1461302
definition: BBa_K1461100
access: public

BBa_E0040
type: Component
persistent identity: component2389774
display id: component2389774
version: 1
top level: BBa_K1461302
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2389772
display id: component2389772
version: 1
top level: BBa_K1461302
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2389772
display id: annotation2389772
version: 1
top level: BBa_K1461302
location: https://synbiohub.org/public/igem/BBa_K1461302/annotation2389772/range2389772/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1461302/annotation2389772/range2389772/1
type: Range
persistent identity: range2389772
display id: range2389772
version: 1
top level: BBa_K1461302
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2389774
display id: annotation2389774
version: 1
top level: BBa_K1461302
location: https://synbiohub.org/public/igem/BBa_K1461302/annotation2389774/range2389774/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1461302/annotation2389774/range2389774/1
type: Range
persistent identity: range2389774
display id: range2389774
version: 1
top level: BBa_K1461302
direction: direction/reverse
start: 19
end: 738
orientation: inline

BBa_K1461100
type: SequenceAnnotation
persistent identity: annotation2389776
display id: annotation2389776
version: 1
top level: BBa_K1461302
location: https://synbiohub.org/public/igem/BBa_K1461302/annotation2389776/range2389776/1
component: BBa_K1461100

https://synbiohub.org/public/igem/BBa_K1461302/annotation2389776/range2389776/1
type: Range
persistent identity: range2389776
display id: range2389776
version: 1
top level: BBa_K1461302
direction: direction/reverse
start: 747
end: 771
orientation: inline

BBa_K1461102
type: SequenceAnnotation
persistent identity: annotation2389778
display id: annotation2389778
version: 1
top level: BBa_K1461302
location: https://synbiohub.org/public/igem/BBa_K1461302/annotation2389778/range2389778/1
component: BBa_K1461102

https://synbiohub.org/public/igem/BBa_K1461302/annotation2389778/range2389778/1
type: Range
persistent identity: range2389778
display id: range2389778
version: 1
top level: BBa_K1461302
direction: direction/reverse
start: 780
end: 802
orientation: inline

BBa_K1461102
type: SequenceAnnotation
persistent identity: annotation2389780
display id: annotation2389780
version: 1
top level: BBa_K1461302
location: https://synbiohub.org/public/igem/BBa_K1461302/annotation2389780/range2389780/1
component: BBa_K1461102

https://synbiohub.org/public/igem/BBa_K1461302/annotation2389780/range2389780/1
type: Range
persistent identity: range2389780
display id: range2389780
version: 1
top level: BBa_K1461302
direction: direction/reverse
start: 811
end: 833
orientation: inline

BBa_K1461102
type: SequenceAnnotation
persistent identity: annotation2389782
display id: annotation2389782
version: 1
top level: BBa_K1461302
location: https://synbiohub.org/public/igem/BBa_K1461302/annotation2389782/range2389782/1
component: BBa_K1461102

https://synbiohub.org/public/igem/BBa_K1461302/annotation2389782/range2389782/1
type: Range
persistent identity: range2389782
display id: range2389782
version: 1
top level: BBa_K1461302
direction: direction/reverse
start: 842
end: 864
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2389789
display id: annotation2389789
version: 1
top level: BBa_K1461302
location: https://synbiohub.org/public/igem/BBa_K1461302/annotation2389789/range2389789/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461302/annotation2389789/range2389789/1
type: Range
persistent identity: range2389789
display id: range2389789
version: 1
top level: BBa_K1461302
direction: direction/reverse
start: 873
end: 1001
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1461100
type: ComponentDefinition
persistent identity: BBa K1461100
display id: BBa_K1461100
version: 1
was generated by: iGEM to SBOL conversion
description: miR-142-3p target binding site
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: 1.Friedland, A. E., Lu, T. K., Wang, X., Shi, D., Church, G., & Collins, J. J. (2009). Synthetic gene networks that count. science, 324(5931), 1199-1202.
owned by: james
owned by: myers
mutable description: After transcription, this cis-repressor sequence makes intramolecular hybridization with RBS and forms stem-loop stem part of which covers RBS. Therefore, when forming stem-loop, following CDS is not translated. However, if the cognate RNA which called taRNA(trans-activating RNA,BBa_k1461103) exists, this cognate RNA in turn makes hybridization with cis-repressor sequence ,undoes stem-loop and exposes RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 16849
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We designed cis-repressor sequence and taRNA sequence to make free energy of most stable formation of stem-loop < free energy of most stable taRNA-annealed RNA by using viennaRNA package.
star: false
creator: Shunsuke Sumi
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: binding
sequence: https://synbiohub.org/public/igem/BBa_K1461100_sequence/1

binding
type: SequenceAnnotation
persistent identity: annotation2386997
display id: annotation2386997
version: 1
top level: BBa_K1461100
location: https://synbiohub.org/public/igem/BBa_K1461100/annotation2386997/range2386997/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1461100/annotation2386997/range2386997/1
type: Range
persistent identity: range2386997
display id: range2386997
version: 1
top level: BBa_K1461100
direction: direction/forward
start: 1
end: 25
orientation: inline

BBa_K1461102
type: ComponentDefinition
persistent identity: BBa K1461102
display id: BBa_K1461102
version: 1
was generated by: iGEM to SBOL conversion
description: miRNA-142 binding site control1
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:33Z
owned by: james
owned by: myers
mutable description: This is meaningless sequence to confirm translation inhibition by miR-142.
discontinued: false
dominant: false
group u list: _1840_
m user id: 0
owner id: 16849
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Shunsuke Sumi
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K1461102_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2387000
display id: annotation2387000
version: 1
top level: BBa_K1461102
location: https://synbiohub.org/public/igem/BBa_K1461102/annotation2387000/range2387000/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1461102/annotation2387000/range2387000/1
type: Range
persistent identity: range2387000
display id: range2387000
version: 1
top level: BBa_K1461102
direction: direction/forward
start: 1
end: 23
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1461308_sequence/1
type: Sequence
persistent identity: BBa K1461308 sequence
display id: BBa_K1461308_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctggtttagtgaaccgtcagatctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagaggtgtagtgtttcctactttatggattactagaggagtagtgctttctactttatgttactagaggagtagtgctttctactttatgttactagaggagtagtgctttctactttatgttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K747096_sequence/1
type: Sequence
persistent identity: BBa K747096 sequence
display id: BBa_K747096_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctggtttagtgaaccgtcagatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461302_sequence/1
type: Sequence
persistent identity: BBa K1461302 sequence
display id: BBa_K1461302_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagaggtgtagtgtttcctactttatggattactagaggagtagtgctttctactttatgttactagaggagtagtgctttctactttatgttactagaggagtagtgctttctactttatgttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461100_sequence/1
type: Sequence
persistent identity: BBa K1461100 sequence
display id: BBa_K1461100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgtagtgtttcctactttatggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461102_sequence/1
type: Sequence
persistent identity: BBa K1461102 sequence
display id: BBa_K1461102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagtagtgctttctactttatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z