LOCUS BBa_K1462150 4091 bp DNA linear 22-Dec-2024 DEFINITION Gal1+AdhE2+GFP+ADH1 ACCESSION BBa_K1462150 VERSION BBa_K1462150.1 FEATURES Location/Qualifiers promoter 1..549 /label=BBa_J63006 CDS 556..3132 /label=BBa_K1462060 CDS 3139..3858 /label=GFP protein CDS 3139..3858 /label=BBa_E0040 misc_feature 3867..4091 /label=ADH1 terminator terminator 3867..4091 /label=BBa_J63002 ORIGIN 1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg 61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg 121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc 181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag 241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa 301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa 361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac 421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa 481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaaactag acccgccgcc 541 accatggagt actagatgaa agtcacaaat caaaaagaat tgaaacagaa attaaatgaa 601 ttgagggaag ctcagaagaa gttcgcaact tatactcaag aacaagttga caaaattttt 661 aaacaatgcg ctattgctgc tgctaaagaa aggataaact tggctaagtt ggctgtcgag 721 gagactggta ttggtttagt tgaagataag ataataaaga atcatttcgc tgctgaatac 781 atttataaca agtacaaaaa cgaaaaaaca tgtggtatta tagatcatga tgattctttg 841 ggtataacaa aagttgctga acctataggt attgtcgctg caatagttcc tactactaat 901 cctacatcta ctgctatttt taagtcatta atttcattaa aaactagaaa cgctatattc 961 ttcagcccac atccaagagc taaaaaatca actattgcag cagctaagtt aattttggat 1021 gcggcggtga aggcaggtgc accaaaaaac attattggat ggattgacga accttctata 1081 gaattgtctc aagatttgat gtcagaggca gacattattt tagctacagg tggtccatca 1141 atggtcaagg ctgcatattc atctggaaag ccagctattg gtgttggtgc tggtaacact 1201 cctgcaatta tagacgagtc agctgatatt gacatggcag tttcttctat aattttgtct 1261 aaaacatatg acaacggtgt tatttgcgca tctgaacaat ctattttggt tatgaacagc 1321 atttatgaaa aagttaagga agagttcgtc aagaggggtt catatatttt gaatcaaaat 1381 gaaattgcta aaattaagga aactatgttc aaaaatggtg caattaacgc tgatattgtt 1441 ggaaagtctg catatattat tgctaagatg gcaggtattg aagtccctca aacaactaag 1501 atattaattg gtgaggtcca gtctgtcgaa aagtctgagt tgttttctca tgaaaaattg 1561 tctccagtct tggctatgta caaagttaaa gacttcgatg aggctttgaa aaaagcacaa 1621 aggttgattg aattaggtgg ttcaggtcat acttcttcat tgtacattga ttctcaaaat 1681 aacaaagata aagttaaaga gtttggttta gcaatgaaaa catcaaggac atttataaac 1741 atgccttcat ctcagggtgc ttctggtgat ttgtataact ttgctattgc tccatcattt 1801 acattgggtt gcggtacttg gggtggaaat tctgtctctc aaaatgttga accaaagcat 1861 ttattaaaca ttaagtctgt tgctgaaaga agagagaata tgttatggtt taaggttcca 1921 caaaaaattt actttaagta tggttgtttg aggtttgcat taaaggaatt gaaggatatg 1981 aacaagaaga gggcatttat tgttacagac aaagatttgt tcaaattagg ttatgttaat 2041 aaaattacta aggttttaga cgaaattgat ataaaatact ctatatttac tgatattaaa 2101 tctgatccaa caattgattc tgttaaaaaa ggagctaaag aaatgttaaa cttcgagcca 2161 gatactatta tttctattgg aggtggttca ccaatggacg cagcaaaggt catgcacttg 2221 ttgtacgaat atccagaggc tgaaattgaa aatttggcta taaattttat ggatataaga 2281 aaaagaattt gcaactttcc taagttaggt acaaaggcta tttctgttgc tattcctact 2341 actgctggaa ctggttctga ggctactcct ttcgctgtta ttacaaatga tgaaacaggt 2401 atgaagtacc cattgacatc ttacgaattg actccaaata tggcaataat tgatacagaa 2461 ttgatgttaa atatgccaag gaagttgact gctgcaacag gtattgatgc attggttcat 2521 gctattgaag cttacgtctc agtcatggca acagactaca ctgatgaatt ggcattaaga 2581 gcaattaaga tgattttcaa atatttacca agagcttaca aaaatggtac taatgatatt 2641 gaagcaagag aaaaaatggc tcatgcttca aacattgctg gtatggcttt cgctaacgct 2701 tttttgggtg tctgccactc tatggctcac aagttgggtg ctatgcacca cgtcccacac 2761 ggtattgctt gcgctgtttt gattgaagaa gttataaagt ataatgctac agattgccct 2821 acaaagcaga ctgcttttcc acagtataag tctcctaacg ctaaaaggaa atatgctgag 2881 atagcagaat atttaaattt aaaaggtaca tctgatactg aaaaggttac tgctttgatt 2941 gaagctattt ctaaattgaa gatagatttg tctattccac aaaatatttc agctgctgga 3001 attaacaaaa aggactttta taacacattg gataagatgt ctgaattagc tttcgatgat 3061 caatgcacta cagctaatcc aaggtatcct ttgatttctg agttgaaaga tatttacatt 3121 aaatcttttt aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 3181 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 3241 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 3301 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 3361 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 3421 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 3481 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 3541 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 3601 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 3661 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 3721 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 3781 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 3841 gaactataca aataataata ctagagtaat aagcgaattt cttatgattt atgattttta 3901 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt 3961 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg 4021 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccgagc aaatgcctgc 4081 aaatcgctcc c //