BBa_K1462310 1 PRK Phosphoribulokinase (from Spinacia oleracea) 2014-10-09T11:00:00Z 2015-05-08T01:10:35Z We got this gene from a synthesis company. The PRK employed in this pathway is from Spinacia oleracea, catalyting the reaction: ATP + D-ribulose 5-phosphate????ADP +??D-ribulose 1,5-bisphosphate false false _1841_ 0 21405 9 Not in stock false The PRK employed in this pathway is from Spinacia oleracea, catalyting the reaction: ATP + D-ribulose 5-phosphate????ADP +??D-ribulose 1,5-bisphosphate false Ye Peng BBa_K801012 1 BBa_K801012 ADH1 yeast terminator 2012-09-20T11:00:00Z 2015-05-08T01:13:24Z Extracted from genomic DNA of ''S. cerevisiae'' using PCR. Released HQ 2013 ADH1 terminator of ''S. cerevisiae'' false false _1057_ 0 13624 9 In stock false - false Georg Sch??tzinger annotation2202145 1 ADH1-Terminator range2202145 1 1 349 BBa_K1462460 1 BBa_K1462460 GBD-lig 2014-10-10T11:00:00Z 2015-05-08T01:10:35Z sss aaa false false _1841_ 0 21371 9 Not in stock false ddd false zhanru Chen annotation2416921 1 GBD ligand range2416921 1 1 96 BBa_J63006 1 GAL1 yeast GAL1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic GAL1 locus Released HQ 2013 GAL1 promoter from S. cerevisiae false true _97_ 0 545 97 In stock false unchanged from genomic sequence true Caroline Ajo-Franklin BBa_K1462320 1 BBa_K1462320 pGAL1+PRK+GBD-ligand+tADH1 2014-10-09T11:00:00Z 2015-05-08T01:10:35Z We got pGAL1 and tADH1 from kits, PRK from a synthesis company and, GBD ligand by overlap PCR. This recombinant consists of four parts, the inducible promoter GAL1, the PRK, the ligand of the scaffold GBD and, the teminator ADH1. false false _1841_ 0 21405 9 It's complicated true PRK is the enzyme that catalytes the reaction from ribulose-5P to ribulose-1,5P, showing high catalytic activity,i.e., a low concentrtion of it is enough for catalyting the reaction. That`s why we utilize a inducible promoter to construct the recombinant. false Ye Peng & Junjie Zhang component2419800 1 BBa_J63006 component2419805 1 BBa_K801012 component2419801 1 BBa_K1462310 component2419803 1 BBa_K1462460 annotation2419801 1 BBa_K1462310 range2419801 1 556 1764 annotation2419803 1 BBa_K1462460 range2419803 1 1773 1868 annotation2419805 1 BBa_K801012 range2419805 1 1877 2225 annotation2419800 1 BBa_J63006 range2419800 1 1 549 BBa_K801012_sequence 1 agctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg BBa_J63006_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag BBa_K1462310_sequence 1 atggctgtttgtactgtctacacaattccaactactactcatttaggttcttctttcaatcagaataataaacaagtcttctttaattacaaaagatcttcttcttctaacaatacattgttcactactagaccatcatatgtcattacttgttcacaacaacaaactattgtcattggtttggctgctgactctggttgcggtaaatctactttcatgagaagattgacatctgtcttcggtggtgctgctgaacctcctaaaggtggtaatccagattcaaacacattgatttctgatacaactactgttatttgtttagatgattttcattctttagatagaaacggtagaaaagtcgaaaaggttactgctttggacccaaaagcaaatgacttcgatttaatgtatgaacaagtcaaggctttgaaggagggtaaggctgtcgacaagccaatatacaaccacgtctctggattgttggacccaccagagttgattcaaccacctaaaattttggttattgagggtttgcacccaatgtacgacgcaagagttagggaattgttggatttttcaatatatttggatatttctaatgaagttaaatttgcatggaaaattcaaagagacatgaaagaaagaggtcactctttagaatctattaaggcttctattgaaagcaggaaaccagattttgatgcttacattgaccctcaaaagcagcacgcagatgtcgtcattgaggtcttgccaacagaattgattcctgatgatgatgaaggtaaggttttgagagttagaatgattcaaaaagaaggtgttaaattttttaacccagtttatttatttgatgaaggttctactatttcttggattccatgtggtagaaaattgacatgttcatacccaggtattaaattctcttacggtccagacactttctacggaaacgaggtcacagtcgttgagatggacggtatgttcgacaggttggacgaattaatttacgttgaatctcatttgtctaacttgtctactaaattttatggtgaagtcacacaacaaatgttgaaacatcaaaattttccaggttcaaataatggtacaggtttctttcaaactattattggtttgaaaattagagatttatttgaacaattggttgcttctaggtcaacagctacagcaacggcggcgaaagcttaa BBa_K1462460_sequence 1 ttggttggtgctttgatgcacgtcatgcagaagaggtctagggctattcactcttctgacgagggtgaggaccaagctggtgatgaagatgaagat BBa_K1462320_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatggctgtttgtactgtctacacaattccaactactactcatttaggttcttctttcaatcagaataataaacaagtcttctttaattacaaaagatcttcttcttctaacaatacattgttcactactagaccatcatatgtcattacttgttcacaacaacaaactattgtcattggtttggctgctgactctggttgcggtaaatctactttcatgagaagattgacatctgtcttcggtggtgctgctgaacctcctaaaggtggtaatccagattcaaacacattgatttctgatacaactactgttatttgtttagatgattttcattctttagatagaaacggtagaaaagtcgaaaaggttactgctttggacccaaaagcaaatgacttcgatttaatgtatgaacaagtcaaggctttgaaggagggtaaggctgtcgacaagccaatatacaaccacgtctctggattgttggacccaccagagttgattcaaccacctaaaattttggttattgagggtttgcacccaatgtacgacgcaagagttagggaattgttggatttttcaatatatttggatatttctaatgaagttaaatttgcatggaaaattcaaagagacatgaaagaaagaggtcactctttagaatctattaaggcttctattgaaagcaggaaaccagattttgatgcttacattgaccctcaaaagcagcacgcagatgtcgtcattgaggtcttgccaacagaattgattcctgatgatgatgaaggtaaggttttgagagttagaatgattcaaaaagaaggtgttaaattttttaacccagtttatttatttgatgaaggttctactatttcttggattccatgtggtagaaaattgacatgttcatacccaggtattaaattctcttacggtccagacactttctacggaaacgaggtcacagtcgttgagatggacggtatgttcgacaggttggacgaattaatttacgttgaatctcatttgtctaacttgtctactaaattttatggtgaagtcacacaacaaatgttgaaacatcaaaattttccaggttcaaataatggtacaggtttctttcaaactattattggtttgaaaattagagatttatttgaacaattggttgcttctaggtcaacagctacagcaacggcggcgaaagcttaatactagagttggttggtgctttgatgcacgtcatgcagaagaggtctagggctattcactcttctgacgagggtgaggaccaagctggtgatgaagatgaagattactagagagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z