LOCUS BBa_K1462400 7159 bp DNA linear 22-Nov-2024 DEFINITION pGAL1+PRK+GBD-ligand+ADH1+pTDH3+RuBisCo+SH3-ligand+ADH1+pTDH3+CA+PD Z-ligand+ADH1 ACCESSION BBa_K1462400 VERSION BBa_K1462400.1 FEATURES Location/Qualifiers promoter 1..549 /label=BBa_J63006 CDS 556..1764 /label=BBa_K1462310 CDS 1773..1868 /label=GBD ligand CDS 1773..1868 /label=BBa_K1462460 misc_feature 1877..2225 /label=ADH1-Terminator terminator 1877..2225 /label=BBa_K801012 promoter 2234..2733 /label=TDH3 Promoter promoter 2234..2733 /label=BBa_K530008 CDS 2740..4119 /label=BBa_K1462330 CDS 4128..4160 /label=SH3 Ligand CDS 4128..4160 /label=BBa_K1462470 misc_feature 4169..4517 /label=ADH1-Terminator terminator 4169..4517 /label=BBa_K801012 promoter 4526..4928 /label=BBa_K165037 CDS 4935..6479 /label=BBa_K1462380 misc_feature 6488..6802 /label=BBa_K1462502 misc_feature 6811..7159 /label=ADH1-Terminator terminator 6811..7159 /label=BBa_K801012 ORIGIN 1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg 61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg 121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc 181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag 241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa 301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa 361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac 421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa 481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaaactag acccgccgcc 541 accatggagt actagatggc tgtttgtact gtctacacaa ttccaactac tactcattta 601 ggttcttctt tcaatcagaa taataaacaa gtcttcttta attacaaaag atcttcttct 661 tctaacaata cattgttcac tactagacca tcatatgtca ttacttgttc acaacaacaa 721 actattgtca ttggtttggc tgctgactct ggttgcggta aatctacttt catgagaaga 781 ttgacatctg tcttcggtgg tgctgctgaa cctcctaaag gtggtaatcc agattcaaac 841 acattgattt ctgatacaac tactgttatt tgtttagatg attttcattc tttagataga 901 aacggtagaa aagtcgaaaa ggttactgct ttggacccaa aagcaaatga cttcgattta 961 atgtatgaac aagtcaaggc tttgaaggag ggtaaggctg tcgacaagcc aatatacaac 1021 cacgtctctg gattgttgga cccaccagag ttgattcaac cacctaaaat tttggttatt 1081 gagggtttgc acccaatgta cgacgcaaga gttagggaat tgttggattt ttcaatatat 1141 ttggatattt ctaatgaagt taaatttgca tggaaaattc aaagagacat gaaagaaaga 1201 ggtcactctt tagaatctat taaggcttct attgaaagca ggaaaccaga ttttgatgct 1261 tacattgacc ctcaaaagca gcacgcagat gtcgtcattg aggtcttgcc aacagaattg 1321 attcctgatg atgatgaagg taaggttttg agagttagaa tgattcaaaa agaaggtgtt 1381 aaatttttta acccagttta tttatttgat gaaggttcta ctatttcttg gattccatgt 1441 ggtagaaaat tgacatgttc atacccaggt attaaattct cttacggtcc agacactttc 1501 tacggaaacg aggtcacagt cgttgagatg gacggtatgt tcgacaggtt ggacgaatta 1561 atttacgttg aatctcattt gtctaacttg tctactaaat tttatggtga agtcacacaa 1621 caaatgttga aacatcaaaa ttttccaggt tcaaataatg gtacaggttt ctttcaaact 1681 attattggtt tgaaaattag agatttattt gaacaattgg ttgcttctag gtcaacagct 1741 acagcaacgg cggcgaaagc ttaatactag agttggttgg tgctttgatg cacgtcatgc 1801 agaagaggtc tagggctatt cactcttctg acgagggtga ggaccaagct ggtgatgaag 1861 atgaagatta ctagagagct ttggacttct tcgccagagg tttggtcaag tctccaatca 1921 aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg 1981 ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta 2041 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt 2101 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg 2161 tatagcatga ggtcgctctt attgaccaca cctctaccgg ccggtcgaaa ttcccctacc 2221 ctatgtacta gagacagttt attcctggca tccactaaat ataatggagc ccgcttttta 2281 agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct tcaccaacca 2341 tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca aacaggcaaa 2401 aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat gacacaaggc 2461 aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct tctgctctct 2521 ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac 2581 ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa tctatttctt 2641 aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac 2701 ttagtttcga ataaacacac ataaacaaac aaatactaga tggatcagtc taacagatat 2761 gctagattgg acttgaaaga ggctgatttg attgctggtg gtaggcacgt cttgtgtgca 2821 tacgtcatga agcctaaggc tggttacggt tacttggaga ctgctgcaca cttcgcagct 2881 gagtcatcta ctggtacaaa cgttgaagtt tcaacaacag atgatttcac taggggtgtc 2941 gacgctttgg tttacgaaat tgatccagaa aaggaaatta tgaaaattgc ttacccagtt 3001 gaattgtttg atagaaatat tattgatggt agagctatgt tgtgctcttt cttgacattg 3061 actattggta acaaccaagg aatgggtgac gtcgaatacg ctaaaatgca tgatttttat 3121 gttccaccat gttacttgag attgttcgat ggtccttcta tgaatattgc tgatatgtgg 3181 agagtcttgg gtagggacgt caggaacggt ggtatggtcg ttggtactat aattaaacca 3241 aagttgggat taaggccaaa accattcgct gacgcttgcc acgagttctg gttgggtgga 3301 gatttcatta aaaatgatga accacaaggt aatcaaactt ttgcaccttt gaaggaaact 3361 attaggttgg ttgcagatgc tatgaagagg gctcaggatg aaactggtga ggctaaatta 3421 ttttcagcta acattactgc tgatgatcac tacgagatgg tcgcaagggg tgagtacatt 3481 ttggaaacat ttggtgaaaa tgctgatcat gttgctttct tggttgatgg ttacgtcact 3541 ggtccagctg ctataactac tgcaaggagg cagttcccaa gacagttctt gcactaccac 3601 agggctggtc acggtgctgt tacttcacca caatctatga gaggttatac tgcttttgtt 3661 ttatcaaaaa tggctaggtt gcagggagct tctggtattc atacaggtac aatgggttac 3721 ggtaagatgg aaggtgaggc tgctgacaag attatggcat acatgttgac agacgaggca 3781 gctgaaggtc cattctacag gcaggactgg ttgggattga aagctacaac tcctattatt 3841 tctggtggta tgaacgcttt aagattgcca ggtttctttg ataatttggg tcattctaac 3901 gtcattcaga catctggtgg tggtgctttc ggtcatttgg acggtggtac ggcgggggca 3961 aagtctttga ggcagtcaca tgaggcatgg atggcaggtg tcgatttggt cacatacgct 4021 agggaacaca gggagttggc aagagcattc gagtcattcc cagcagatgc tgacaagttc 4081 tacccaggtt ggagggatag attgcagagg gctgcttaat actagagcca ccaccagctt 4141 tgccaccaaa gagaagaaga tactagagag ctttggactt cttcgccaga ggtttggtca 4201 agtctccaat caaggttgtc ggcttgtcta ccttgccaga aatttacgaa aagatggaaa 4261 agggtcaaat cgttggtaga tacgttgttg acacttctaa ataagcgaat ttcttatgat 4321 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg 4381 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt 4441 tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc ggccggtcga 4501 aattccccta ccctatgtac tagagattac ccataaggtt gtttgtgacg gcgtcgtaca 4561 agagaacgtg ggaacttttt aggctcacca aaaaagaaag aaaaaatacg agttgctgac 4621 agaagcctca agaaaaaaaa aattcttctt cgactatgct ggaggcagag atgatcgagc 4681 cggtagttaa ctatatatag ctaaattggt tccatcacct tcttttctgg tgtcgctcct 4741 tctagtgcta tttctggctt ttcctatttt tttttttcca tttttctttc tctctttcta 4801 atatataaat tctcttgcat tttctatttt tctctctatc tattctactt gtttattccc 4861 ttcaaggttt ttttttaagg agtacttgtt tttagaatat acggtcaacg aactataatt 4921 aactaaacta ctagatgaat acaagaaata ctagatctaa gcagagagct ccatgcggtg 4981 tttcatcttc tgtcaagcct agattggatt tgattgaaca agctccaaac ccagtctacg 5041 atagacaccc agcatgcatt actttgccag agaggacttg cagacaccca ttaactgatt 5101 tagaggctaa cgaacaattg ggtaggtgcg aggattctgt taagaacaga tttgatagag 5161 ttataccatt cttgcaggtc gtcgcaggta ttccattagg attggattac gttactagag 5221 ttcaagaatt ggcacaatct tctttgggtc atacattacc tgaagaattg ttgaaggata 5281 attggatttc tggtcataat ttgaaaggta ttttcggata cgctactgca aaggctttga 5341 cagctgctac agaacaattt agcaggaaga taatgtcaga aaaagacgac tcagcttctg 5401 ctattgggtt cttcctggac tgtggtttcc acgctgtcga catttcacca tgcgcagacg 5461 gtagattgaa gggattgttg ccatacattt tgagattacc attgacagct tttacatata 5521 ggaaggctta tgcaggttct atgttcgata ttgaagacga tttagctcaa tgggagaaga 5581 acgagttgag aagatacagg gagggtgtcc caaacacggc ggaccagcca actagatatt 5641 taaaaattgc tgtttatcac ttctctacat ctgacccaac acactcaggt tgcgctgctc 5701 acggttctaa cgacagagct gctttggaag ctgctttgac tcagttgatg aaatttaggg 5761 aagcagttga gaacgctcac tgctgcggtg cttctattga tattttgttg attggtgttg 5821 atacagatac agatgctata agagttcaca ttcctgattc taaaggtttc ttgaatccat 5881 atagatatgt tgacaacact gttacttatg ctcaaacttt gcacttggca ccagacgaag 5941 ctagggtcat tattcacgaa gcaattttga atgctaacag atctgatggt tgggctaagg 6001 gtaacggtgt cgcatcagag ggtatgagaa gatttatagg tcaattatta attaacaatt 6061 tgtctcaaat tgattacgtc gttaataggc acggtggtag gtacccacca aacgacattg 6121 gtcatgctga aagatatatt tctgtcggtg acggttttga cgaggtccaa attagaaatt 6181 tggcttatta tgcacatttg gatactgtcg aagaaaacgc tattgatgtt gatgtcggta 6241 ttactatttt tacaaaattg aacttgagca ggggtttgcc aattccaatt gctattcact 6301 atagatatga tccaaatgtt cctggtagca gggagaggac agtcgtcaag gctagaagaa 6361 tttataatgc tattaaggaa agattttctt ctttagatga acaaaactta ttgcaattca 6421 gattgtctgt tcaagctcaa gatataggtt ctcctattga agaagttgct tctgcttaat 6481 actagagttg caaagaagaa gagttactgt cagaaaggct gacgctggtg gtttgggtat 6541 ttctattaaa ggtggtagag aaaataaaat gccaattttg atttctaaaa ttttcaaggg 6601 tttggctgct gaccagactg aggctttgtt cgttggtgat gctattttgt ctgttaatgg 6661 tgaagacttg tcttctgcta ctcacgacga ggcagtccaa gctttgaaga agactggtaa 6721 agaagttgtt ttggaagtta aatatatgaa agaagtttct ccatatttta aggctggctc 6781 gagtgctgct ggttctggat cctactagag agctttggac ttcttcgcca gaggtttggt 6841 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga 6901 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg 6961 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag 7021 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag 7081 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggccggtc 7141 gaaattcccc taccctatg //