This is the complete molecule for pathway engineering of outer mitochondrial membrane.
RuBisCo (from Thiobacillus denitrificans)
This RuBisCo, cbbs, is a prokaryotic form-Ⅱ RuBisCo from Thiobacillus denitrificans and used for catalyting the reaction: Ribulose 1,5-bisphosphate + CO2 2x 3-phosphoglycerate
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
Phosphoribulokinase (from Spinacia oleracea)
We got this gene from a synthesis company.
The PRK employed in this pathway is from Spinacia oleracea, catalyting the reaction: ATP + D-ribulose 5-phosphate????ADP +??D-ribulose 1,5-bisphosphate
TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
Promoter was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the promoter.
Carbonic anhydrase (from Halothiobacillus neapolitanus c2)
The CA we use is a component of the carboxysome shell of Halothiobacillus neapolitanus c2.
From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
This is a high level constitutive promoter from the yeast gene TEF2.
Conversion of the iGEM parts registry to SBOL2.1