LOCUS BBa_K1462410 10648 bp DNA linear 22-Nov-2024 DEFINITION pTEF2+GroES+tADH1+pTEF2+GroEL+tADH1+pGAL1+PRK+GBD-ligand+tADH1+pTDH 3+RuBisCo+SH3-ligand+tADH1+pTDH3+ ACCESSION BBa_K1462410 VERSION BBa_K1462410.1 FEATURES Location/Qualifiers promoter 1..403 /label=BBa_K165037 CDS 410..2056 /label=BBa_K1462980 misc_feature 2065..2413 /label=ADH1-Terminator terminator 2065..2413 /label=BBa_K801012 promoter 2422..2824 /label=BBa_K165037 CDS 2831..3124 /label=BBa_K1462990 misc_feature 3133..3481 /label=ADH1-Terminator terminator 3133..3481 /label=BBa_K801012 promoter 3490..4038 /label=BBa_J63006 CDS 4045..5253 /label=BBa_K1462310 CDS 5262..5357 /label=GBD ligand CDS 5262..5357 /label=BBa_K1462460 misc_feature 5366..5714 /label=ADH1-Terminator terminator 5366..5714 /label=BBa_K801012 promoter 5723..6222 /label=TDH3 Promoter promoter 5723..6222 /label=BBa_K530008 CDS 6229..7608 /label=BBa_K1462330 CDS 7617..7649 /label=SH3 Ligand CDS 7617..7649 /label=BBa_K1462470 misc_feature 7658..8006 /label=ADH1-Terminator terminator 7658..8006 /label=BBa_K801012 promoter 8015..8417 /label=BBa_K165037 CDS 8424..9968 /label=BBa_K1462380 misc_feature 9977..10291 /label=BBa_K1462502 misc_feature 10300..10648 /label=ADH1-Terminator terminator 10300..10648 /label=BBa_K801012 ORIGIN 1 attacccata aggttgtttg tgacggcgtc gtacaagaga acgtgggaac tttttaggct 61 caccaaaaaa gaaagaaaaa atacgagttg ctgacagaag cctcaagaaa aaaaaaattc 121 ttcttcgact atgctggagg cagagatgat cgagccggta gttaactata tatagctaaa 181 ttggttccat caccttcttt tctggtgtcg ctccttctag tgctatttct ggcttttcct 241 attttttttt ttccattttt ctttctctct ttctaatata taaattctct tgcattttct 301 atttttctct ctatctattc tacttgttta ttcccttcaa ggtttttttt taaggagtac 361 ttgtttttag aatatacggt caacgaacta taattaacta aactactaga tggctgctaa 421 ggatgttaag tttggtaatg acgctagagt taaaatgttg agaggtgtca acgtcttggc 481 agacgcagtc aaggttactt tgggtccaaa aggtaggaac gtcgtcttgg acaagtcatt 541 cggagctcct acaataacaa aggacggtgt ttctgttgct agagaaatag aattagaaga 601 taaattcgaa aatatgggtg cacaaatggt taaagaagtc gcttcaaagg caaacgatgc 661 tgctggtgac ggtactacaa ctgctactgt cttggcacaa gctattatta cagaaggttt 721 gaaagcagtc gctgctggta tgaacccaat ggatttaaaa agaggtattg acaaggctgt 781 cacagctgct gtcgaggagt taaaggcttt gtcagttcca tgttcagatt ctaaagctat 841 tgcacaagtt ggtactatat ctgcaaattc tgacgaaaca gttggtaagt taattgcaga 901 agcaatggat aaagttggta aagaaggtgt cattacagtc gaggacggta ctggtttgca 961 ggacgagttg gatgttgtcg aaggaatgca atttgataga ggttacttat caccatattt 1021 cattaataag cctgagactg gtgctgtcga attggaatct ccttttattt tgttggctga 1081 caagaagata tctaatatta gagaaatgtt gcctgtcttg gaggctgtcg caaaagcagg 1141 taagccattg ttgattattg cagaagatgt tgaaggtgaa gctttggcaa cattagttgt 1201 caacacaatg agaggtattg ttaaggtcgc tgctgtcaag gcacctggtt tcggtgatag 1261 gagaaaggca atgttgcagg atatagctac tttgacaggt ggaacagtta tttcagaaga 1321 aataggtatg gagttggaga aggctacttt ggaggacttg ggtcaggcta aaagggttgt 1381 cattaataaa gatacaacaa ctattattga tggtgttggt gaggaagctg ctattcaggg 1441 tagggtcgca cagattaggc agcagattga ggaggcaaca tcagactacg acagggagaa 1501 gttacaggag agggttgcta agttggcagg tggtgttgct gtcattaagg tcggtgcagc 1561 tactgaggtt gagatgaagg agaagaaggc tagggttgag gacgcattac acgctactag 1621 agcagctgtt gaggagggtg ttgttgctgg tggtggtgtc gctttgataa gagtcgcttc 1681 taaattggct gacttgagag gtcaaaatga agatcagaac gttggtatta aggttgcatt 1741 gagggctatg gaagctccat tgagacaaat tgttttgaat tgcggagaag aaccatcagt 1801 tgtcgcaaac acagtcaagg gtggtgatgg taactatggt tacaatgctg ctactgaaga 1861 gtatggtaat atgatagata tgggtatttt agatccaaca aaagtcacta ggtctgcttt 1921 gcagtatgct gcttctgtcg caggattgat gattacaact gaatgcatgg tcactgactt 1981 gccaaaaaac gacgcggcgg acttgggtgc agcaggtggt atgggtggta tgggtggtat 2041 gggtggtatg atgtaatact agagagcttt ggacttcttc gccagaggtt tggtcaagtc 2101 tccaatcaag gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg 2161 tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat 2221 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc 2281 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct 2341 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggcc ggtcgaaatt 2401 cccctaccct atgtactaga gattacccat aaggttgttt gtgacggcgt cgtacaagag 2461 aacgtgggaa ctttttaggc tcaccaaaaa agaaagaaaa aatacgagtt gctgacagaa 2521 gcctcaagaa aaaaaaaatt cttcttcgac tatgctggag gcagagatga tcgagccggt 2581 agttaactat atatagctaa attggttcca tcaccttctt ttctggtgtc gctccttcta 2641 gtgctatttc tggcttttcc tatttttttt tttccatttt tctttctctc tttctaatat 2701 ataaattctc ttgcattttc tatttttctc tctatctatt ctacttgttt attcccttca 2761 aggttttttt ttaaggagta cttgttttta gaatatacgg tcaacgaact ataattaact 2821 aaactactag atgaatatta gacctttgca tgatagagtt attgttaaaa gaaaagaagt 2881 tgaaactaaa tctgctggtg gtattgtctt gactggttct gctgctgcta agtctactag 2941 gggtgaggtc ttggctgttg gtaatggtag aattttggaa aatggtgaag ttaaaccatt 3001 ggatgttaaa gttggtgata ttgttatttt taatgatggt tatggtgtta aatctgaaaa 3061 aattgataat gaagaagttt tgattatgtc tgaatctgat attttggcta ttgttgaagc 3121 ttaatactag agagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt 3181 tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg 3241 tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat 3301 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa 3361 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata 3421 gcatgaggtc gctcttattg accacacctc taccggccgg tcgaaattcc cctaccctat 3481 gtactagagc cccattatct tagcctaaaa aaaccttctc tttggaactt tcagtaatac 3541 gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg ggtgacagcc 3601 ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc 3661 agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact agcttttatg 3721 gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat gaacgaatca 3781 aattaacaac cataggatga taatgcgatt agttttttag ccttatttct ggggtaatta 3841 atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa actgcataac 3901 cactttaact aatactttca acattttcgg tttgtattac ttcttattca aatgtaataa 3961 aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga ggaaactaga 4021 cccgccgcca ccatggagta ctagatggct gtttgtactg tctacacaat tccaactact 4081 actcatttag gttcttcttt caatcagaat aataaacaag tcttctttaa ttacaaaaga 4141 tcttcttctt ctaacaatac attgttcact actagaccat catatgtcat tacttgttca 4201 caacaacaaa ctattgtcat tggtttggct gctgactctg gttgcggtaa atctactttc 4261 atgagaagat tgacatctgt cttcggtggt gctgctgaac ctcctaaagg tggtaatcca 4321 gattcaaaca cattgatttc tgatacaact actgttattt gtttagatga ttttcattct 4381 ttagatagaa acggtagaaa agtcgaaaag gttactgctt tggacccaaa agcaaatgac 4441 ttcgatttaa tgtatgaaca agtcaaggct ttgaaggagg gtaaggctgt cgacaagcca 4501 atatacaacc acgtctctgg attgttggac ccaccagagt tgattcaacc acctaaaatt 4561 ttggttattg agggtttgca cccaatgtac gacgcaagag ttagggaatt gttggatttt 4621 tcaatatatt tggatatttc taatgaagtt aaatttgcat ggaaaattca aagagacatg 4681 aaagaaagag gtcactcttt agaatctatt aaggcttcta ttgaaagcag gaaaccagat 4741 tttgatgctt acattgaccc tcaaaagcag cacgcagatg tcgtcattga ggtcttgcca 4801 acagaattga ttcctgatga tgatgaaggt aaggttttga gagttagaat gattcaaaaa 4861 gaaggtgtta aattttttaa cccagtttat ttatttgatg aaggttctac tatttcttgg 4921 attccatgtg gtagaaaatt gacatgttca tacccaggta ttaaattctc ttacggtcca 4981 gacactttct acggaaacga ggtcacagtc gttgagatgg acggtatgtt cgacaggttg 5041 gacgaattaa tttacgttga atctcatttg tctaacttgt ctactaaatt ttatggtgaa 5101 gtcacacaac aaatgttgaa acatcaaaat tttccaggtt caaataatgg tacaggtttc 5161 tttcaaacta ttattggttt gaaaattaga gatttatttg aacaattggt tgcttctagg 5221 tcaacagcta cagcaacggc ggcgaaagct taatactaga gttggttggt gctttgatgc 5281 acgtcatgca gaagaggtct agggctattc actcttctga cgagggtgag gaccaagctg 5341 gtgatgaaga tgaagattac tagagagctt tggacttctt cgccagaggt ttggtcaagt 5401 ctccaatcaa ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg 5461 gtcaaatcgt tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta 5521 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact 5581 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc 5641 tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggc cggtcgaaat 5701 tcccctaccc tatgtactag agacagttta ttcctggcat ccactaaata taatggagcc 5761 cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt 5821 caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa 5881 acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg 5941 acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt 6001 ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta 6061 ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat 6121 ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac 6181 accaagaact tagtttcgaa taaacacaca taaacaaaca aatactagat ggatcagtct 6241 aacagatatg ctagattgga cttgaaagag gctgatttga ttgctggtgg taggcacgtc 6301 ttgtgtgcat acgtcatgaa gcctaaggct ggttacggtt acttggagac tgctgcacac 6361 ttcgcagctg agtcatctac tggtacaaac gttgaagttt caacaacaga tgatttcact 6421 aggggtgtcg acgctttggt ttacgaaatt gatccagaaa aggaaattat gaaaattgct 6481 tacccagttg aattgtttga tagaaatatt attgatggta gagctatgtt gtgctctttc 6541 ttgacattga ctattggtaa caaccaagga atgggtgacg tcgaatacgc taaaatgcat 6601 gatttttatg ttccaccatg ttacttgaga ttgttcgatg gtccttctat gaatattgct 6661 gatatgtgga gagtcttggg tagggacgtc aggaacggtg gtatggtcgt tggtactata 6721 attaaaccaa agttgggatt aaggccaaaa ccattcgctg acgcttgcca cgagttctgg 6781 ttgggtggag atttcattaa aaatgatgaa ccacaaggta atcaaacttt tgcacctttg 6841 aaggaaacta ttaggttggt tgcagatgct atgaagaggg ctcaggatga aactggtgag 6901 gctaaattat tttcagctaa cattactgct gatgatcact acgagatggt cgcaaggggt 6961 gagtacattt tggaaacatt tggtgaaaat gctgatcatg ttgctttctt ggttgatggt 7021 tacgtcactg gtccagctgc tataactact gcaaggaggc agttcccaag acagttcttg 7081 cactaccaca gggctggtca cggtgctgtt acttcaccac aatctatgag aggttatact 7141 gcttttgttt tatcaaaaat ggctaggttg cagggagctt ctggtattca tacaggtaca 7201 atgggttacg gtaagatgga aggtgaggct gctgacaaga ttatggcata catgttgaca 7261 gacgaggcag ctgaaggtcc attctacagg caggactggt tgggattgaa agctacaact 7321 cctattattt ctggtggtat gaacgcttta agattgccag gtttctttga taatttgggt 7381 cattctaacg tcattcagac atctggtggt ggtgctttcg gtcatttgga cggtggtacg 7441 gcgggggcaa agtctttgag gcagtcacat gaggcatgga tggcaggtgt cgatttggtc 7501 acatacgcta gggaacacag ggagttggca agagcattcg agtcattccc agcagatgct 7561 gacaagttct acccaggttg gagggataga ttgcagaggg ctgcttaata ctagagccac 7621 caccagcttt gccaccaaag agaagaagat actagagagc tttggacttc ttcgccagag 7681 gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa 7741 agatggaaaa gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt 7801 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat 7861 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt 7921 aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg 7981 gccggtcgaa attcccctac cctatgtact agagattacc cataaggttg tttgtgacgg 8041 cgtcgtacaa gagaacgtgg gaacttttta ggctcaccaa aaaagaaaga aaaaatacga 8101 gttgctgaca gaagcctcaa gaaaaaaaaa attcttcttc gactatgctg gaggcagaga 8161 tgatcgagcc ggtagttaac tatatatagc taaattggtt ccatcacctt cttttctggt 8221 gtcgctcctt ctagtgctat ttctggcttt tcctattttt ttttttccat ttttctttct 8281 ctctttctaa tatataaatt ctcttgcatt ttctattttt ctctctatct attctacttg 8341 tttattccct tcaaggtttt tttttaagga gtacttgttt ttagaatata cggtcaacga 8401 actataatta actaaactac tagatgaata caagaaatac tagatctaag cagagagctc 8461 catgcggtgt ttcatcttct gtcaagccta gattggattt gattgaacaa gctccaaacc 8521 cagtctacga tagacaccca gcatgcatta ctttgccaga gaggacttgc agacacccat 8581 taactgattt agaggctaac gaacaattgg gtaggtgcga ggattctgtt aagaacagat 8641 ttgatagagt tataccattc ttgcaggtcg tcgcaggtat tccattagga ttggattacg 8701 ttactagagt tcaagaattg gcacaatctt ctttgggtca tacattacct gaagaattgt 8761 tgaaggataa ttggatttct ggtcataatt tgaaaggtat tttcggatac gctactgcaa 8821 aggctttgac agctgctaca gaacaattta gcaggaagat aatgtcagaa aaagacgact 8881 cagcttctgc tattgggttc ttcctggact gtggtttcca cgctgtcgac atttcaccat 8941 gcgcagacgg tagattgaag ggattgttgc catacatttt gagattacca ttgacagctt 9001 ttacatatag gaaggcttat gcaggttcta tgttcgatat tgaagacgat ttagctcaat 9061 gggagaagaa cgagttgaga agatacaggg agggtgtccc aaacacggcg gaccagccaa 9121 ctagatattt aaaaattgct gtttatcact tctctacatc tgacccaaca cactcaggtt 9181 gcgctgctca cggttctaac gacagagctg ctttggaagc tgctttgact cagttgatga 9241 aatttaggga agcagttgag aacgctcact gctgcggtgc ttctattgat attttgttga 9301 ttggtgttga tacagataca gatgctataa gagttcacat tcctgattct aaaggtttct 9361 tgaatccata tagatatgtt gacaacactg ttacttatgc tcaaactttg cacttggcac 9421 cagacgaagc tagggtcatt attcacgaag caattttgaa tgctaacaga tctgatggtt 9481 gggctaaggg taacggtgtc gcatcagagg gtatgagaag atttataggt caattattaa 9541 ttaacaattt gtctcaaatt gattacgtcg ttaataggca cggtggtagg tacccaccaa 9601 acgacattgg tcatgctgaa agatatattt ctgtcggtga cggttttgac gaggtccaaa 9661 ttagaaattt ggcttattat gcacatttgg atactgtcga agaaaacgct attgatgttg 9721 atgtcggtat tactattttt acaaaattga acttgagcag gggtttgcca attccaattg 9781 ctattcacta tagatatgat ccaaatgttc ctggtagcag ggagaggaca gtcgtcaagg 9841 ctagaagaat ttataatgct attaaggaaa gattttcttc tttagatgaa caaaacttat 9901 tgcaattcag attgtctgtt caagctcaag atataggttc tcctattgaa gaagttgctt 9961 ctgcttaata ctagagttgc aaagaagaag agttactgtc agaaaggctg acgctggtgg 10021 tttgggtatt tctattaaag gtggtagaga aaataaaatg ccaattttga tttctaaaat 10081 tttcaagggt ttggctgctg accagactga ggctttgttc gttggtgatg ctattttgtc 10141 tgttaatggt gaagacttgt cttctgctac tcacgacgag gcagtccaag ctttgaagaa 10201 gactggtaaa gaagttgttt tggaagttaa atatatgaaa gaagtttctc catattttaa 10261 ggctggctcg agtgctgctg gttctggatc ctactagaga gctttggact tcttcgccag 10321 aggtttggtc aagtctccaa tcaaggttgt cggcttgtct accttgccag aaatttacga 10381 aaagatggaa aagggtcaaa tcgttggtag atacgttgtt gacacttcta aataagcgaa 10441 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa 10501 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct 10561 gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac 10621 cggccggtcg aaattcccct accctatg //