promoter TEF2 + chaperon GroES + terminator ADH1 + promoter TEF2 + chaperon GroEL + terminator ADH1 + promoter GAL1+PRK+ligand of the scaffold GBD+ terminator ADH1 promoter TDH3+RuBisCo+ligand of the scaffold SH3+ terminator ADH1
We got this gene from a synthesis company.
It`s one of the E.coli protein-folding chaperons. It was optimized to be RFC10 and RFC23 compatible.
It`s one of the E.coli protein-folding chaperons.
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
This is a high level constitutive promoter from the yeast gene TEF2.
RuBisCo (from Thiobacillus denitrificans)
This RuBisCo, cbbs, is a prokaryotic form-Ⅱ RuBisCo from Thiobacillus denitrificans and used for catalyting the reaction: Ribulose 1,5-bisphosphate + CO2 2x 3-phosphoglycerate
Phosphoribulokinase (from Spinacia oleracea)
The PRK employed in this pathway is from Spinacia oleracea, catalyting the reaction: ATP + D-ribulose 5-phosphate????ADP +??D-ribulose 1,5-bisphosphate
TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
Promoter was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the promoter.
Conversion of the iGEM parts registry to SBOL2.1