BBa_K1463345
type: ComponentDefinition
persistent identity: BBa K1463345
display id: BBa_K1463345
version: 1
was generated by: iGEM to SBOL conversion
description: GvpA and GvpC in J61002 Vector
created: 2014-08-14T11:00:00Z
modified: 2015-05-08T01:10:37Z
mutable provenance: All parts are in the 2014 distribution.
owned by: james
owned by: myers
mutable description: This part contains GvpA with a strong RBS, B0034, and GvpC with a medium RBS, B0032. Both of which are under a constitutive promoter, J23100. This construct is in J61002.
discontinued: false
dominant: false
group u list: _1842_
m user id: 0
owner id: 23036
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No specific design considerations.
star: false
creator: Beth Greig
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K737016
component: BBa_B0034
component: BBa_K737017
component: BBa_B0032
component: BBa_J23100
component: BBa_J61002
sequence annotation: BBa_J61002
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_K737016
sequence annotation: BBa_B0032
sequence annotation: BBa_K737017
sequence: https://synbiohub.org/public/igem/BBa_K1463345_sequence/1

BBa_K737016
type: Component
persistent identity: component2381060
display id: component2381060
version: 1
top level: BBa_K1463345
definition: BBa_K737016
access: public

BBa_B0034
type: Component
persistent identity: component2381059
display id: component2381059
version: 1
top level: BBa_K1463345
definition: BBa_B0034
access: public

BBa_K737017
type: Component
persistent identity: component2381064
display id: component2381064
version: 1
top level: BBa_K1463345
definition: BBa_K737017
access: public

BBa_B0032
type: Component
persistent identity: component2381062
display id: component2381062
version: 1
top level: BBa_K1463345
definition: BBa_B0032
access: public

BBa_J23100
type: Component
persistent identity: component2381057
display id: component2381057
version: 1
top level: BBa_K1463345
definition: BBa_J23100
access: public

BBa_J61002
type: Component
persistent identity: component2381056
display id: component2381056
version: 1
top level: BBa_K1463345
definition: BBa_J61002
access: public

BBa_J61002
type: SequenceAnnotation
persistent identity: annotation2381056
display id: annotation2381056
version: 1
top level: BBa_K1463345
location: https://synbiohub.org/public/igem/BBa_K1463345/annotation2381056/range2381056/1
component: BBa_J61002

https://synbiohub.org/public/igem/BBa_K1463345/annotation2381056/range2381056/1
type: Range
persistent identity: range2381056
display id: range2381056
version: 1
top level: BBa_K1463345
direction: direction/reverse
start: 1
end: 2948
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2381057
display id: annotation2381057
version: 1
top level: BBa_K1463345
location: https://synbiohub.org/public/igem/BBa_K1463345/annotation2381057/range2381057/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1463345/annotation2381057/range2381057/1
type: Range
persistent identity: range2381057
display id: range2381057
version: 1
top level: BBa_K1463345
direction: direction/reverse
start: 2957
end: 2991
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2381059
display id: annotation2381059
version: 1
top level: BBa_K1463345
location: https://synbiohub.org/public/igem/BBa_K1463345/annotation2381059/range2381059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1463345/annotation2381059/range2381059/1
type: Range
persistent identity: range2381059
display id: range2381059
version: 1
top level: BBa_K1463345
direction: direction/reverse
start: 3000
end: 3011
orientation: inline

BBa_K737016
type: SequenceAnnotation
persistent identity: annotation2381060
display id: annotation2381060
version: 1
top level: BBa_K1463345
location: https://synbiohub.org/public/igem/BBa_K1463345/annotation2381060/range2381060/1
component: BBa_K737016

https://synbiohub.org/public/igem/BBa_K1463345/annotation2381060/range2381060/1
type: Range
persistent identity: range2381060
display id: range2381060
version: 1
top level: BBa_K1463345
direction: direction/reverse
start: 3018
end: 3236
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2381062
display id: annotation2381062
version: 1
top level: BBa_K1463345
location: https://synbiohub.org/public/igem/BBa_K1463345/annotation2381062/range2381062/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1463345/annotation2381062/range2381062/1
type: Range
persistent identity: range2381062
display id: range2381062
version: 1
top level: BBa_K1463345
direction: direction/forward
start: 3245
end: 3257
orientation: inline

BBa_K737017
type: SequenceAnnotation
persistent identity: annotation2381064
display id: annotation2381064
version: 1
top level: BBa_K1463345
location: https://synbiohub.org/public/igem/BBa_K1463345/annotation2381064/range2381064/1
component: BBa_K737017

https://synbiohub.org/public/igem/BBa_K1463345/annotation2381064/range2381064/1
type: Range
persistent identity: range2381064
display id: range2381064
version: 1
top level: BBa_K1463345
direction: direction/reverse
start: 3264
end: 3767
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_J61002
type: ComponentDefinition
persistent identity: BBa J61002
display id: BBa_J61002
version: 1
was generated by: iGEM to SBOL conversion
description: EX-Ptet-S-rbsRFP-P "RFP reporter"
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: PCR ca1020F/R on pSB1A2-I13521 (900bp, SpeI/PstI) Sub into pSB1A2-I13522 (SpeI/PstI) Product is pBca1020, will be an iGEM number ultimately ---- ca1020F Forward SpeI oligo for X-rbsRFP-P plasmid gcactACTAGTgaaagaggagaaatactagatggc ca1020R Reverse PstI oligo for X-rbsRFP-P plasmid ccggactgcagcggccgcttctagtatataaacg
owned by: james
owned by: myers
mutable description: Plasmid for construction of promoter basic parts. Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: SpeI
sequence annotation: BBa_B0034 RBS
sequence annotation: RBS
sequence annotation: mRFP
sequence annotation: BBa_B0015
sequence annotation: rrnB terminator
sequence annotation: NotI
sequence annotation: PstI
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: ColE1 origin
sequence annotation: pBR322 origin
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: beta-lactamase (ampR, bla) promoter
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: BioBrick prefix
sequence annotation: Ambiguity; should be a T
sequence: https://synbiohub.org/public/igem/BBa_J61002_sequence/1

SpeI
type: SequenceAnnotation
persistent identity: annotation1975716
display id: annotation1975716
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975716/range1975716/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J61002/annotation1975716/range1975716/1
type: Range
persistent identity: range1975716
display id: range1975716
version: 1
top level: BBa_J61002
direction: direction/forward
start: 2
end: 7
orientation: inline

BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975767
display id: annotation1975767
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975767/range1975767/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J61002/annotation1975767/range1975767/1
type: Range
persistent identity: range1975767
display id: range1975767
version: 1
top level: BBa_J61002
direction: direction/forward
start: 9
end: 20
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975766
display id: annotation1975766
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975766/range1975766/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J61002/annotation1975766/range1975766/1
type: Range
persistent identity: range1975766
display id: range1975766
version: 1
top level: BBa_J61002
direction: direction/forward
start: 9
end: 20
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation1892273
display id: annotation1892273
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892273/range1892273/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J61002/annotation1892273/range1892273/1
type: Range
persistent identity: range1892273
display id: range1892273
version: 1
top level: BBa_J61002
direction: direction/forward
start: 27
end: 707
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1892274
display id: annotation1892274
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892274/range1892274/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J61002/annotation1892274/range1892274/1
type: Range
persistent identity: range1892274
display id: range1892274
version: 1
top level: BBa_J61002
direction: direction/forward
start: 741
end: 869
orientation: inline

rrnB terminator
type: SequenceAnnotation
persistent identity: annotation1975713
display id: annotation1975713
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975713/range1975713/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_J61002/annotation1975713/range1975713/1
type: Range
persistent identity: range1975713
display id: range1975713
version: 1
top level: BBa_J61002
direction: direction/forward
start: 752
end: 795
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975718
display id: annotation1975718
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975718/range1975718/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_J61002/annotation1975718/range1975718/1
type: Range
persistent identity: range1975718
display id: range1975718
version: 1
top level: BBa_J61002
direction: direction/forward
start: 878
end: 885
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1975717
display id: annotation1975717
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975717/range1975717/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_J61002/annotation1975717/range1975717/1
type: Range
persistent identity: range1975717
display id: range1975717
version: 1
top level: BBa_J61002
direction: direction/forward
start: 885
end: 890
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975768
display id: annotation1975768
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975768/range1975768/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J61002/annotation1975768/range1975768/1
type: Range
persistent identity: range1975768
display id: range1975768
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 948
end: 967
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1892275
display id: annotation1892275
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892275/range1892275/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J61002/annotation1892275/range1892275/1
type: Range
persistent identity: range1892275
display id: range1892275
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 1049
end: 1731
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975714
display id: annotation1975714
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975714/range1975714/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J61002/annotation1975714/range1975714/1
type: Range
persistent identity: range1975714
display id: range1975714
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 1052
end: 1671
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1892276
display id: annotation1892276
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892276/range1892276/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J61002/annotation1892276/range1892276/1
type: Range
persistent identity: range1892276
display id: range1892276
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 1826
end: 2686
orientation: inline

beta-lactamase (ampR, bla) promoter
type: SequenceAnnotation
persistent identity: annotation1892272
display id: annotation1892272
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892272/range1892272/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J61002/annotation1892272/range1892272/1
type: Range
persistent identity: range1892272
display id: range1892272
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 2728
end: 2756
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975769
display id: annotation1975769
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975769/range1975769/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J61002/annotation1975769/range1975769/1
type: Range
persistent identity: range1975769
display id: range1975769
version: 1
top level: BBa_J61002
direction: direction/forward
start: 2809
end: 2828
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1975715
display id: annotation1975715
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975715/range1975715/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J61002/annotation1975715/range1975715/1
type: Range
persistent identity: range1975715
display id: range1975715
version: 1
top level: BBa_J61002
direction: direction/forward
start: 2927
end: 2948
orientation: inline

Ambiguity; should be a T
type: SequenceAnnotation
persistent identity: annotation1975921
display id: annotation1975921
version: 1
top level: BBa_J61002
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975921/range1975921/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J61002/annotation1975921/range1975921/1
type: Range
persistent identity: range1975921
display id: range1975921
version: 1
top level: BBa_J61002
direction: direction/forward
start: 2941
end: 2941
orientation: inline

BBa_K737016
type: ComponentDefinition
persistent identity: BBa K737016
display id: BBa_K737016
version: 1
was generated by: iGEM to SBOL conversion
description: This part conteins gvpA protein's coding sequence.
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
mutable provenance: Yes.
owned by: james
owned by: myers
mutable description: This part conteins gvpA protein's coding sequence. This gene from Planktothrix rubescens can be succesfully expressed in E. coli. The gvpA's expression have been examined by another part " J23106+B0034+gvpA+B0015". (Since it was ligated on cloning vector pSB4A5, we didn't register this part. The gvpA protein conteins a typical domain of the superfamily 'Gas vesicle'(NCBI CDD cl02594). This gene is quiet conserved in most gvp's polycistron. According to Anthony E. Walsby's research, gas vesicles have a similar morphology. GvpA protein is a kind of small hydrophobic protein which forms the ribs of the main structure. 'All cyanobacterial gas vesicles so far analyzed contain a protein (GvpA) of about 7.4 kDa that forms the main mass of the structure and must be responsible for many of its properties.' (Gas Vesicles. Microbiological Reviews. A. E. Walsby)
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: According to Anthony E. Walsby's research, gas vesicles have a similar morphology. GvpA protein is a kind of small hydrophobic protein which forms the ribs of the main structure. 'All cyanobacterial gas vesicles so far analyzed contain a protein (GvpA) of about 7.4 kDa that forms the main mass of the structure and must be responsible for many of its properties.' (Gas Vesicles. Microbiological Reviews. A. E. Walsby)
star: false
creator: Jiaheng Li
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K737016_sequence/1

BBa_K737017
type: ComponentDefinition
persistent identity: BBa K737017
display id: BBa_K737017
version: 1
was generated by: iGEM to SBOL conversion
description: This part conteins gvpC-20psi protein
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:07Z
mutable provenance: Yes
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part conteins gvpA protein's coding sequence. This gene from Planktothrix rubescens can be succesfully expressed in E. coli. The gvpA's expression have been examined by another part " J23106+B0034+gvpA+B0015". (Since it was ligated on cloning vector pSB4A5, we didn't register this part. The gvpA protein conteins a typical domain of the superfamily 'Gas vesicle'(NCBI CDD cl02594). This gene is quiet conserved in most gvp's polycistron. According to Anthony E. Walsby's research, gas vesicles have a similar morphology. GvpA protein is a kind of small hydrophobic protein which forms the ribs of the main structure. 'All cyanobacterial gas vesicles so far analyzed contain a protein (GvpA) of about 7.4 kDa that forms the main mass of the structure and must be responsible for many of its properties.' (Gas Vesicles. Microbiological Reviews. A. E. Walsby)
discontinued: false
dominant: false
experience: experience/Works
group u list: _986_
m user id: 0
owner id: 14291
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The gvpA protein conteins a typical domain of the superfamily 'Gas vesicle'(NCBI CDD cl02594). This gene is quiet conserved in most gvp's polycistron. According to Anthony E. Walsby's research, gas vesicles have a similar morphology. GvpA protein is a kind of small hydrophobic protein which forms the ribs of the main structure. 'All cyanobacterial gas vesicles so far analyzed contain a protein (GvpA) of about 7.4 kDa that forms the main mass of the structure and must be responsible for many of its properties.' (Gas Vesicles. Microbiological Reviews. A. E. Walsby)
star: false
creator: Jiaheng Li
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K737017_sequence/1

https://synbiohub.org/public/igem/BBa_K1463345_sequence/1
type: Sequence
persistent identity: BBa K1463345 sequence
display id: BBa_K1463345_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61002_sequence/1
type: Sequence
persistent identity: BBa J61002 sequence
display id: BBa_J61002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K737016_sequence/1
type: Sequence
persistent identity: BBa K737016 sequence
display id: BBa_K737016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgttgaaaaagtaaactcatcctccagtctggccgaagttatcgatcggatcttagataaaggcattgtgattgacgcttgggtacgggtttccctcgttggaatcgagcttctatccatagaagcaagaatcgtgatcgcttctgttgaaacctatctcaagtacgcagaagccgttggtttaaccgcacaggcggctgttccttcggtctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K737017_sequence/1
type: Sequence
persistent identity: BBa K737017 sequence
display id: BBa_K737017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctttaaaagacaagtggcaacaggatcgtatcggacgccaacagggagttcaagaacggcaacagcaagttcaaaccaccctatccctctggcaacaagagcgccaaaatcaggcttctgaatttcgggaagacctagaatatcgggtaacggatctgttagctaattatcagaaacagcgcctagaagctagggaaactttacttgaggacttagctatttttcgtcaaaccctatatcgggaagtcgaagaatatttaggggagttagatattctgcaccagcaaatggccgcacaattacaacaacaactccaacagagtcggacggaaagaaaagacgctgttcagaagttattcgaggatttaggggtatttcgcgccgaactacaagactatcacctcaaacttcaacagacagtttgggggagttcccaccgaaaaccgcgaaaagcgattaccccgcaacgctctattccatcgcgtttatattcctgttaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z