LOCUS BBa_K1463805 3703 bp DNA linear 11-May-2024 DEFINITION RFP under J23103 promoter ACCESSION BBa_K1463805 VERSION BBa_K1463805.1 FEATURES Location/Qualifiers promoter 2728..2756 /label=beta-lactamase (ampR, bla) promoter CDS 27..707 /label=mRFP misc_feature 741..869 /label=BBa_B0015 misc_feature 1049..1731 /label=ColE1 origin CDS 1826..2686 /label=beta-lactamase ampicillin resistance marker stem_loop 752..795 /label=rrnB terminator misc_feature 1052..1671 /label=pBR322 origin misc_feature 2927..2948 /label=BioBrick prefix misc_feature 2..7 /label=SpeI misc_feature 885..890 /label=PstI misc_feature 878..885 /label=NotI RBS 9..20 /label=RBS misc_feature 9..20 /label=BBa_B0034 RBS primer_bind 948..967 /label=Verification reverse (VR) primer binding site primer_bind 2809..2828 /label=Verification forward (VF2) primer binding site misc_feature 2941..2941 /label=Ambiguity; should be a T misc_feature 1..2948 /label=BBa_J61002 promoter 2957..2991 /label=BBa_J23103 CDS 2998..3672 /label=mrfp1 misc_feature 3679..3703 /label=Help:Barcodes CDS 2998..3703 /label=BBa_E1010 ORIGIN 1 tactagtgaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca 61 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg 121 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg 181 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt 241 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca 301 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct 361 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg 421 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc 481 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc 541 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg 601 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg 661 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct 721 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 781 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 841 accttcgggt gggcctttct gcgtttatat actagaagcg gccgctgcag gcttcctcgc 901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 1021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 1081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 1141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 1201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 1261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 1321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 1381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 1441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 1501 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 1561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 1621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 1681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 1741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 1801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 1861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 1921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 1981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 2041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 2101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 2161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 2221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 2281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 2341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 2401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 2461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 2521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 2581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 2641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 2701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 2761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 2821 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga 2881 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 2941 atctagagta ctagagctga tagctagctc agtcctaggg attatgctag ctactagatg 3001 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc 3061 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc 3121 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg 3181 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac 3241 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac 3301 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa 3361 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg 3421 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc 3481 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac 3541 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc 3601 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac 3661 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgc //