LOCUS BBa_K1465102 3350 bp DNA linear 22-Dec-2024 DEFINITION Fumarate reductase (frd) from E. coli under the control of the T7 promoter ACCESSION BBa_K1465102 VERSION BBa_K1465102.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 promotor (BBa_K525998) RBS 21..32 /label=RBS (BBa_B0034) CDS 33..3350 /label=Fumerate reductase (frd) ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat gcaaaccttt caagccgatc 61 ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa 121 atccgaatgc aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc cataccgttg 181 cagcagaagg gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa tatcactttc 241 acgatacagt agcgggtggc gactggttgt gtgagcagga tgtcgtggat tatttcgtcc 301 accactgccc aaccgaaatg acccaactgg aactgtgggg atgcccatgg agccgtcgcc 361 cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg 421 ccgccgataa gaccggcttc catatgctgc acacgctgtt ccagacctct ctgcaattcc 481 cgcagatcca gcgttttgac gaacatttcg tgctggatat tctggttgat gatggtcatg 541 ttcgcggcct ggtagcaatg aacatgatgg aaggcacgct ggtgcagatc cgtgctaacg 601 cggtcgttat ggctactggc ggtgcgggtc gcgtttatcg ttacaacacc aacggcggca 661 tcgttaccgg tgacggtatg ggtatggcgc taagccacgg cgttccgctg cgtgacatgg 721 aatttgttca gtatcaccca accggtctgc caggttccgg tatcctgatg accgaaggtt 781 gccgcggtga aggcggtatt ctggtcaaca aaaatggcta ccgttatctg caagattacg 841 gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac 901 gcgacaaagt ctctcaggcc ttctggcacg aatggcgtaa aggcaacacc atctccacgc 961 cgcgtggcga tgtggtttat ctcgacttgc gtcacctcgg cgagaaaaaa ctgcatgaac 1021 gtctgccgtt catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac 1081 cgattccggt acgtccgacc gcacactaca ccatgggcgg tatcgaaacc gatcagaact 1141 gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg 1201 gtgcaaaccg tctgggttct aactccctgg cggaactggt ggtcttcggc cgtctggccg 1261 gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagcg gcaattgaag 1321 cgcaggcagc tggcgttgaa caacgtctga aagatctggt taaccaggat ggcggcgaaa 1381 actgggcgaa gatccgcgac gaaatgggcc tggctatgga agaaggctgc ggtatctacc 1441 gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcaa gaacgcttca 1501 agcgcgtgcg catcaccgac acttccagcg tgttcaacac cgacctgctc tacaccattg 1561 aactgggcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag 1621 agtcccgcgg cgcgcaccag cgtctggacg aaggttgcac cgagcgtgac gacgtcaact 1681 tcctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg gagtacagcg 1741 acgtgaagat tactacgctg ccgccagcta aacgcgttta cggtggcgaa gcggatgcag 1801 ccgataaggc ggaagcagcc aataagaagg agaaggcgaa tggctgagat gaaaaacctg 1861 aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag cgcattctat 1921 gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat caaagacaac 1981 ctggcaccgg acctgagcta ccgctggtcc tgccgtatgg cgatttgtgg ttcctgcggc 2041 atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga ttacaccgac 2101 ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg 2161 acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc 2221 gcggatcagg gtactaacat ccagaccccg gcgcagatgg cgaagtatca ccagttctcc 2281 ggttgcatca actgtggttt gtgctacgcc gcgtgcccgc agtttggcct gaacccagag 2341 ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac 2401 ggtaagaagg agcgtatggc gcagttgaac agccagaacg gcgtatggag ctgtactttc 2461 gtgggctact gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag 2521 ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg 2581 caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa 2641 attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt 2701 cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat ggcccggaag cctgggcggg 2761 attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca ctctggcggc 2821 agctctgctg cacaccaaaa cctggtttga actggcaccg aaagcggcca atatcattgt 2881 aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt 2941 tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc 3001 caaatccaaa gcgttctgac gaaccggtat tctggggcct cttcggggcc ggtggtatgt 3061 ggagcgccat cattgcgccg gtgatgatcc tgctggtggg tattctgctg ccactggggt 3121 tgtttccggg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg 3181 gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgtatgc 3241 accacgcgat gcacgatctg aaaatccacg tacctgcggg caaatgggtt ttctacggtc 3301 tggctgctat cctgacagtt gtcacgctga ttggtgtcgt tacaatctaa //