LOCUS       BBa_K1465105            3381 bp    DNA     linear       22-Nov-2024
DEFINITION  Fumarate reductase (Fum) under control of the T7 promoter
ACCESSION   BBa_K1465105
VERSION     BBa_K1465105.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     RBS             32..43
                     /label=B0034
     CDS             44..1834
                     /label=Fumerate reductase FumA
     misc_feature    32..1834
                     /label=BBa_K1465103
     RBS             1843..1854
                     /label=B0034
     CDS             1855..2622
                     /label=FumB
     RBS             2623..2634
                     /label=B0034
     CDS             2635..3024
                     /label=FumC
     RBS             3025..3036
                     /label=B0034
     CDS             3037..3381
                     /label=FumD
     misc_feature    1843..3381
                     /label=BBa_K1465104
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaaatgcaga cagttaacgt
       61 cgatatcgcc atcgtgggcg ccggtggtgg cggcctgcgt gctgcgattg cggctgcgga
      121 agcgaaccct aacctgaaaa ttgccctggt gtcgaaagtc tacccgatgc gctctcacac
      181 cgtcgcggcc gaaggtggag ctgctgctgt tattaaagaa gaagattctt atgataaaca
      241 ctttcaggac acagtcgctg gtggcgattg gctgtgcgag caggatgttg ttgagtattt
      301 tgttaaacac tccccggtgg aaatgaccca actggagcgt tggggttgtc cttggagccg
      361 caaagcggat ggtgatgtaa acgtgcgtcg tttcgggggt atgaagattg aacgtacatg
      421 gttcgcggca gataaaactg gctttcacat gcttcacacg ctgttccaaa cgagcattca
      481 gtatccgcag attcagcgct tcgatgagca ctttgttctc gacattttgg tggatgatgg
      541 ccatgcccgt gggatggtag cgatgaacat gatggaaggc accttggttc agatcaatgc
      601 aaatgcggta gttattgcga ccggaggcgg atgtcgctct tttaaattta ataccaatgg
      661 tggtattgtt accggcgatg gcctgtccat ggcatatcgt cacggagttc cgctgcgcga
      721 catggagttt gttcaatatc accccacggg gctgccgaac acggggatcc tgatgacgga
      781 aggatgtcgc ggggaaggcg gcatcctggt taacaaagat ggttatcgtt acctccagga
      841 ttacggactt ggcccggaaa cgccgattgg taaaccgcag aataaatata tggaactggg
      901 cccccgcgat aaagtgtcac aggcattttg gcaggaatgg aaaaaaggta atactttaaa
      961 aaccgcgaaa ggcgttgatg tggttcatct ggacctgcgt catctgggtg cggattactt
     1021 gcacgagcgc ttacctttca tttgcgaatt ggcgaatgcg tatgaaggcg tagatccggt
     1081 gaaagagccg atcccggttc gtccagtcgt tcattatact atgggaggaa ttgaaatcga
     1141 ttttaattcg gagacccgca tcaaagggct gttcgctgtc ggagaatgtg catcgtcagg
     1201 cctccatggc gcgaatcgcc tgggttctaa ctcgctggcc gaattagtgg tactgggtcg
     1261 tgttgcaggt gaacatgcag cgcaacgtgc caccgaagcc cagtcggtta accaagctgc
     1321 tgttgacgcg caagcgcgtg acgtcgttaa acgcctggag gatttgtata atcaagaagg
     1381 taccgaaaat tgggctgata ttcgtgagga gatggggacc gcaatggagg aaggttgcgg
     1441 gatttaccgc gacgaggcca gcatgcagac tgcggtgaac aaaatcgcgg aactcaagga
     1501 gcgctataaa cgtattcgca ttcaggaccg ttcatcagtt tttaatacga atgttctgta
     1561 taccgttgaa ctgggctata ttcttgatgt cgcccaaagc attgcgaact ctgcattaca
     1621 acgcaaagag agccgcggtg cccaccagcg ccttgactac accgaacgtg acgatgtcaa
     1681 ctatctgaaa catacactcg ccttctacaa tggaaacgat gctccgcgca ttgactatag
     1741 cgacgtgaaa atcacgacgt ttcagcccgc taagcgtgtg tacggcgccg aagctgaagc
     1801 tcaggaaaaa gccgccaaag cgccgaatgc gtaatactag agaaagagga gaaaatgacc
     1861 aaccaagtta tgaatgtgga aatcctgcgc tataacccgg aaactgataa agaaccgtat
     1921 cttaagacct accaggttcc ttatgataac cagacctctc tcttggatgc attgggctac
     1981 attaaagatc gcctggagcc ggaactgtct tatcgctggt cttgtcgcat ggcaatctgt
     2041 ggttcttgcg gaatgatggt gaacaacatt ccgaaattgg catgtaaaac ttttctgcgc
     2101 gactactctg gccatatgcg tattgaagcg cttgccaatt ttccgatcga gcgcgacctg
     2161 gttgtggatt tgtcacattt tattgaaagc ttggaggcca ttaaacccta catcatcggc
     2221 aataagatgc ccgagttaga tgggcagcct cacgaatccg cggaactgca aaaatcacgc
     2281 acgaaacaga ccccaaagca gcttgaaaaa tatcgtcagt tctctatgtg cattaattgt
     2341 ggcctctgtt atgcggcatg tccgcaattt gggttaaatc ccgaatttgt tggcccggca
     2401 gctctgacca tggcacatcg ctataacctg gataaccgcg ataacggtaa agcagagcgc
     2461 atgccgatta tcaatggcga taatggtgtt tggacatgca ccttcgtcgg tgcgtgtagc
     2521 gaagtttgcc ctaaacacgt gaatccagcc gcggccatta accaaggcaa actcgagtcc
     2581 gccaaagatt ttctcatttc catgctgaaa ccaaaagcat aaaaagagga gaaaatgtct
     2641 gaacttacta ccaagcgtaa taaatatgtg cgtgaaatta ccccgacgtg gtggaaaagc
     2701 tgggatttct accgctttta tatggtgcgc gaggcaacag cagttccgac cgtttggttc
     2761 tgtcttgttt tgctttatgg tgtcttctcg cttgctgata acacatttgc caccgacttc
     2821 gtgggattca tgcagaaccc gatcgttatg atcctcaata tcatcagtct ggctgccatg
     2881 ctgttgcatg cctttacgct ctttacgatg accggtgaag ttatgtctaa cagcacggga
     2941 ctgaaagcag aagtgattaa gaaggcactt caaatcgctt ttgcagtggt ctctgtcatc
     3001 ggcttgattc tggcttttat ctgaaaagag gagaaaatga ttaacaccaa cccgaaacgt
     3061 agtaatgaac caccggtatg gcttctgttc agcgcgggag gtatgatcag cgcgttggcc
     3121 tttcctgtgc tgatcttaat tctcggcatc ttacttccct taggtattat tagcccggat
     3181 gggattatcg cgttcgcaca ccattggttc ggcaaactgg tgatcttggt gttgaccatc
     3241 tttccggcat gggcgggtct gcaccgcatt catcatggta tgcatgacat caaagtgcat
     3301 gtgccatcgg gtggactgat cttttacggt ctggccgtgc tgtacactgt agtcgcgatc
     3361 tggggtgtgg cgtcgatcta a
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