LOCUS BBa_K1465214 4066 bp DNA linear 26-Dec-2024 DEFINITION RubisCO of Halothiobacillus neapolitanus and phosphoribulokinase A (prkA) under control of ptac ACCESSION BBa_K1465214 VERSION BBa_K1465214.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=stop CDS 1..1084 /label=LacI misc_feature 1081..1084 /label=start RBS 1085..1092 /label=rbs promoter 1093..1153 /label=LacIq promoter misc_feature 1..1153 /label=BBa_K731300 misc_feature 1194..1219 /label=lacO operator promoter 1162..1188 /label=Ptac promoter promoter 1..1239 /label=BBa_K731500 RBS 1248..1259 /label=BBa_B0034 CDS 1266..3038 /label=Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) misc_feature 1248..3038 /label=BBa_K1465202 misc_feature 1..3038 /label=BBa_K1465213 RBS 3047..3058 /label=B0034 CDS 3065..4066 /label=Phosphoribulokinase prkA misc_feature 3047..4066 /label=BBa_K1465201 ORIGIN 1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga 121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc 181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata 241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag 301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat 361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc 421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg 481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat 541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga 601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt 661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag 721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct 781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc 841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa 901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat 961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg 1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt 1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt 1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt 1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagaaa gaggagaaat 1261 actagatggc tgaaatgcag gattacaagc aaagcctcaa atatgagact ttctcttatc 1321 ttccacccat gaacgcggaa cgcatccgcg ctcaaatcaa gtacgcaatt gctcaaggct 1381 ggagccccgg cattgagcac gtagaagtga aaaactccat gaaccaatat tggtacatgt 1441 ggaaacttcc cttcttcggc gaacaaaatg tcgacaacgt gttggctgaa attgaagcgt 1501 gtcgtagtgc gtatccaaca caccaggtca aactggtggc ttatgacaac tatgcgcaaa 1561 gcttaggtct ggccttcgtg gtctaccgcg gcaactaaaa agaggagaaa tactagatgg 1621 cagttaagaa atatagcgcg ggtgtcaaag agtatcgcca gacctactgg atgccagaat 1681 acacaccgct ggattcggat attctggcat gttttaaaat taccccacag cctggagtgg 1741 accgcgagga agctgcggct gctgtcgcgg cggaatccag tacaggtacg tggactacag 1801 tttggaccga ccttctgaca gatatggact attataaagg ccgcgcatat cgcattgaag 1861 acgttcccgg ggatgatgcg gcattttatg cttttatcgc ataccctatt gatctgtttg 1921 aagaggggag cgtagtgaat gtgttcacct cattggtggg taacgtcttc ggatttaagg 1981 cggttcgcgg tcttcgcttg gaagatgtcc gctttccatt ggcgtacgta aagacttgcg 2041 gggggccgcc gcacgggatc caagtcgaac gtgataaaat gaacaaatac gggcgtccat 2101 tattaggttg tacgatcaaa ccgaaactgg gtctgtcggc caaaaattac ggccgcgctg 2161 tgtatgaatg cttgcgcgga ggcctggatt tcactaaaga tgacgagaat atcaacagcc 2221 agccgttcat gcgctggcgc gatcgcttcc tgtttgtcca agacgccaca gaaacagccg 2281 aagcccagac cggggaacgc aaagggcatt atcttaacgt tacggcacct actccggaag 2341 agatgtacaa acgcgccgaa tttgcgaaag agatcggtgc tcctatcatt atgcacgatt 2401 acatcacagg cggattcact gccaacacgg gcctggcgaa atggtgtcag gacaacggtg 2461 tcctgcttca catccaccgg gcaatgcatg ctgtcatcga tcgcaatccg aaccacggta 2521 ttcattttcg tgtgctgacg aagattcttc gcctgtccgg aggagatcac ctgcataccg 2581 ggactgtggt tggcaaatta gagggcgatc gtgctagcac actgggatgg attgacctgc 2641 tgcgggagag cttcattcca gaagaccgct ctcgtggcat ttttttcgac caagactggg 2701 gtagcatgcc aggagttttc gcggtggcgt cgggtgggat ccatgtgtgg cacatgccag 2761 cgctggtgaa tatttttgga gatgacagcg tcttgcaatt cggaggcggt acgctggggc 2821 atccgtgggg aaacgccgcg ggtgcagcag ccaaccgggt cgcattagaa gcatgcgtcg 2881 aagcacgcaa tcagggtcgc gatattgaaa aagagggcaa agaaatcctc acggcagcag 2941 cacagcattc tccggaactg aagatcgcaa tggagacatg gaaggaaatt aaattcgagt 3001 ttgataccgt ggataagttg gacacacaaa accgttaata ctagagaaag aggagaaata 3061 ctagatgagc aagccagatc gtgttgttct gatcggcgtt gccggtgact ccggttgcgg 3121 caagtcaacc ttcctaaacc gccttgccga cctgtttggt accgaactga tgaccgttat 3181 ctgcctggat gactatcact ctctggatcg caagggccgt aaggaagcag gcgtaaccgc 3241 tctggatccg cgcgccaaca actttgacct gatgtatgaa caggttaagg cgctgaagaa 3301 cggcgaaacc atcatgaagc cgatctacaa ccatgaaacc ggcctgatcg atccgccgga 3361 aaaaatcgaa ccgaaccgca tcattgtgat cgagggtctg catccgcttt acgacgagcg 3421 cgtgcgtgaa ctgctggatt tcagcgttta cctggacatc gatgacgaag ttaaaatcgc 3481 ttggaagatc caacgcgata tggcagaacg cggccactcc tacgaagatg ttctggcctc 3541 tatcgaagcg cgccgcccgg acttcaaggc ctacattgag ccgcagcgtg gccatgcgga 3601 catcgttatc cgcgttatgc cgacccagct aatcccgaac gacaccgagc gcaaggtgct 3661 gcgtgtgcag ctgatccaac gtgaaggccg cgatggtttt gagccggctt acctgttcga 3721 cgaaggttct accatccagt ggaccccgtg cggtcgtaag ctgacatgct cctatccggg 3781 cattcgctta gcctacggcc cggacaccta ctacggtcac gaagtttcag tgcttgaggt 3841 tgacggtcag ttcgagaacc tggaagagat gatctacgtt gagggccacc tgagcaagac 3901 cgacacccag tactacggtg agctgaccca cctgctgctg caacacaaag attacccggg 3961 ttctaacaac ggcaccggtc tgttccaagt gctgaccggc ctgaaaatgc gtgcggccta 4021 tgagcgtctg acctcccaag cagcaccggt tgccgctagc gtttaa //