BBa_K1465214 1 BBa_K1465214 RubisCO of Halothiobacillus neapolitanus and phosphoribulokinase A (prkA) under control of ptac 2014-10-08T11:00:00Z 2015-05-08T01:10:38Z dsg vgfdh false false _1844_ 0 20850 9 It's complicated false sdfsdf false iGEM-Team Bielefeld 2014 component2416995 1 BBa_K1465201 component2416992 1 BBa_K1465213 annotation2416992 1 BBa_K1465213 range2416992 1 1 3038 annotation2416995 1 BBa_K1465201 range2416995 1 3047 4066 BBa_K731500 1 BBa_K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward 2012-08-21T11:00:00Z 2015-05-08T01:13:06Z pET21b This is E.coli lactose-inducibile promoter. lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow. false false _977_ 0 12142 9 In stock true false Francesco Guzzonato annotation2181543 1 LacIq promoter range2181543 1 1093 1153 annotation2181546 1 Ptac promoter range2181546 1 1162 1188 annotation2181545 1 lacO operator range2181545 1 1194 1219 annotation2181539 1 stop range2181539 1 1 3 annotation2181544 1 BBa_K731300 range2181544 1 1 1153 annotation2181541 1 start range2181541 1 1081 1084 annotation2181540 1 LacI range2181540 1 1 1084 annotation2181542 1 rbs range2181542 1 1085 1092 BBa_K1465202 1 BBa_K1465202 Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Halothiobacillus neapolitanus 2014-09-01T11:00:00Z 2015-05-08T01:10:37Z PCR-Amplifikation using the primer Phosphoribulokinase prkA from S. elangatus with RBS BBa_B0034 false false _1844_ 0 16986 9 It's complicated true ha false iGEM-Team Bielefeld 2014 annotation2382590 1 BBa_B0034 range2382590 1 1 12 annotation2382591 1 Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) range2382591 1 19 1791 BBa_K1465201 1 BBa_K1465201 Phosphoribulokinase prkA from Synechococcus elongatus 2014-08-12T11:00:00Z 2015-05-08T01:10:37Z Gensynthesis Phosphoribulokinase prkA from Synechoccus elongatus, codon optimized for ''E. coli'' false false _1844_ 0 16986 9 It's complicated false Gibson-Assembly false iGEM-Team Bielefeld 2014 annotation2385326 1 Phosphoribulokinase prkA range2385326 1 19 1020 annotation2380923 1 B0034 range2380923 1 1 12 BBa_K1465213 1 BBa_K1465213 RubisCO of Halothiobacillus neapolitanus under control of the ptac promoter 2014-10-01T11:00:00Z 2015-05-08T01:10:38Z bb Hneap RubisCO under control of the ptac promoter false false _1844_ 0 16986 9 In stock false bb false iGEM-Team Bielefeld 2014 component2392393 1 BBa_K1465202 component2392390 1 BBa_K731500 annotation2392390 1 BBa_K731500 range2392390 1 1 1239 annotation2392393 1 BBa_K1465202 range2392393 1 1248 3038 BBa_K1465202_sequence 1 aaagaggagaaatactagatggctgaaatgcaggattacaagcaaagcctcaaatatgagactttctcttatcttccacccatgaacgcggaacgcatccgcgctcaaatcaagtacgcaattgctcaaggctggagccccggcattgagcacgtagaagtgaaaaactccatgaaccaatattggtacatgtggaaacttcccttcttcggcgaacaaaatgtcgacaacgtgttggctgaaattgaagcgtgtcgtagtgcgtatccaacacaccaggtcaaactggtggcttatgacaactatgcgcaaagcttaggtctggccttcgtggtctaccgcggcaactaaaaagaggagaaatactagatggcagttaagaaatatagcgcgggtgtcaaagagtatcgccagacctactggatgccagaatacacaccgctggattcggatattctggcatgttttaaaattaccccacagcctggagtggaccgcgaggaagctgcggctgctgtcgcggcggaatccagtacaggtacgtggactacagtttggaccgaccttctgacagatatggactattataaaggccgcgcatatcgcattgaagacgttcccggggatgatgcggcattttatgcttttatcgcataccctattgatctgtttgaagaggggagcgtagtgaatgtgttcacctcattggtgggtaacgtcttcggatttaaggcggttcgcggtcttcgcttggaagatgtccgctttccattggcgtacgtaaagacttgcggggggccgccgcacgggatccaagtcgaacgtgataaaatgaacaaatacgggcgtccattattaggttgtacgatcaaaccgaaactgggtctgtcggccaaaaattacggccgcgctgtgtatgaatgcttgcgcggaggcctggatttcactaaagatgacgagaatatcaacagccagccgttcatgcgctggcgcgatcgcttcctgtttgtccaagacgccacagaaacagccgaagcccagaccggggaacgcaaagggcattatcttaacgttacggcacctactccggaagagatgtacaaacgcgccgaatttgcgaaagagatcggtgctcctatcattatgcacgattacatcacaggcggattcactgccaacacgggcctggcgaaatggtgtcaggacaacggtgtcctgcttcacatccaccgggcaatgcatgctgtcatcgatcgcaatccgaaccacggtattcattttcgtgtgctgacgaagattcttcgcctgtccggaggagatcacctgcataccgggactgtggttggcaaattagagggcgatcgtgctagcacactgggatggattgacctgctgcgggagagcttcattccagaagaccgctctcgtggcatttttttcgaccaagactggggtagcatgccaggagttttcgcggtggcgtcgggtgggatccatgtgtggcacatgccagcgctggtgaatatttttggagatgacagcgtcttgcaattcggaggcggtacgctggggcatccgtggggaaacgccgcgggtgcagcagccaaccgggtcgcattagaagcatgcgtcgaagcacgcaatcagggtcgcgatattgaaaaagagggcaaagaaatcctcacggcagcagcacagcattctccggaactgaagatcgcaatggagacatggaaggaaattaaattcgagtttgataccgtggataagttggacacacaaaaccgttaa BBa_K731500_sequence 1 tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgttt BBa_K1465213_sequence 1 tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgttttactagagaaagaggagaaatactagatggctgaaatgcaggattacaagcaaagcctcaaatatgagactttctcttatcttccacccatgaacgcggaacgcatccgcgctcaaatcaagtacgcaattgctcaaggctggagccccggcattgagcacgtagaagtgaaaaactccatgaaccaatattggtacatgtggaaacttcccttcttcggcgaacaaaatgtcgacaacgtgttggctgaaattgaagcgtgtcgtagtgcgtatccaacacaccaggtcaaactggtggcttatgacaactatgcgcaaagcttaggtctggccttcgtggtctaccgcggcaactaaaaagaggagaaatactagatggcagttaagaaatatagcgcgggtgtcaaagagtatcgccagacctactggatgccagaatacacaccgctggattcggatattctggcatgttttaaaattaccccacagcctggagtggaccgcgaggaagctgcggctgctgtcgcggcggaatccagtacaggtacgtggactacagtttggaccgaccttctgacagatatggactattataaaggccgcgcatatcgcattgaagacgttcccggggatgatgcggcattttatgcttttatcgcataccctattgatctgtttgaagaggggagcgtagtgaatgtgttcacctcattggtgggtaacgtcttcggatttaaggcggttcgcggtcttcgcttggaagatgtccgctttccattggcgtacgtaaagacttgcggggggccgccgcacgggatccaagtcgaacgtgataaaatgaacaaatacgggcgtccattattaggttgtacgatcaaaccgaaactgggtctgtcggccaaaaattacggccgcgctgtgtatgaatgcttgcgcggaggcctggatttcactaaagatgacgagaatatcaacagccagccgttcatgcgctggcgcgatcgcttcctgtttgtccaagacgccacagaaacagccgaagcccagaccggggaacgcaaagggcattatcttaacgttacggcacctactccggaagagatgtacaaacgcgccgaatttgcgaaagagatcggtgctcctatcattatgcacgattacatcacaggcggattcactgccaacacgggcctggcgaaatggtgtcaggacaacggtgtcctgcttcacatccaccgggcaatgcatgctgtcatcgatcgcaatccgaaccacggtattcattttcgtgtgctgacgaagattcttcgcctgtccggaggagatcacctgcataccgggactgtggttggcaaattagagggcgatcgtgctagcacactgggatggattgacctgctgcgggagagcttcattccagaagaccgctctcgtggcatttttttcgaccaagactggggtagcatgccaggagttttcgcggtggcgtcgggtgggatccatgtgtggcacatgccagcgctggtgaatatttttggagatgacagcgtcttgcaattcggaggcggtacgctggggcatccgtggggaaacgccgcgggtgcagcagccaaccgggtcgcattagaagcatgcgtcgaagcacgcaatcagggtcgcgatattgaaaaagagggcaaagaaatcctcacggcagcagcacagcattctccggaactgaagatcgcaatggagacatggaaggaaattaaattcgagtttgataccgtggataagttggacacacaaaaccgttaa BBa_K1465201_sequence 1 aaagaggagaaatactagatgagcaagccagatcgtgttgttctgatcggcgttgccggtgactccggttgcggcaagtcaaccttcctaaaccgccttgccgacctgtttggtaccgaactgatgaccgttatctgcctggatgactatcactctctggatcgcaagggccgtaaggaagcaggcgtaaccgctctggatccgcgcgccaacaactttgacctgatgtatgaacaggttaaggcgctgaagaacggcgaaaccatcatgaagccgatctacaaccatgaaaccggcctgatcgatccgccggaaaaaatcgaaccgaaccgcatcattgtgatcgagggtctgcatccgctttacgacgagcgcgtgcgtgaactgctggatttcagcgtttacctggacatcgatgacgaagttaaaatcgcttggaagatccaacgcgatatggcagaacgcggccactcctacgaagatgttctggcctctatcgaagcgcgccgcccggacttcaaggcctacattgagccgcagcgtggccatgcggacatcgttatccgcgttatgccgacccagctaatcccgaacgacaccgagcgcaaggtgctgcgtgtgcagctgatccaacgtgaaggccgcgatggttttgagccggcttacctgttcgacgaaggttctaccatccagtggaccccgtgcggtcgtaagctgacatgctcctatccgggcattcgcttagcctacggcccggacacctactacggtcacgaagtttcagtgcttgaggttgacggtcagttcgagaacctggaagagatgatctacgttgagggccacctgagcaagaccgacacccagtactacggtgagctgacccacctgctgctgcaacacaaagattacccgggttctaacaacggcaccggtctgttccaagtgctgaccggcctgaaaatgcgtgcggcctatgagcgtctgacctcccaagcagcaccggttgccgctagcgtttaa BBa_K1465214_sequence 1 tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgttttactagagaaagaggagaaatactagatggctgaaatgcaggattacaagcaaagcctcaaatatgagactttctcttatcttccacccatgaacgcggaacgcatccgcgctcaaatcaagtacgcaattgctcaaggctggagccccggcattgagcacgtagaagtgaaaaactccatgaaccaatattggtacatgtggaaacttcccttcttcggcgaacaaaatgtcgacaacgtgttggctgaaattgaagcgtgtcgtagtgcgtatccaacacaccaggtcaaactggtggcttatgacaactatgcgcaaagcttaggtctggccttcgtggtctaccgcggcaactaaaaagaggagaaatactagatggcagttaagaaatatagcgcgggtgtcaaagagtatcgccagacctactggatgccagaatacacaccgctggattcggatattctggcatgttttaaaattaccccacagcctggagtggaccgcgaggaagctgcggctgctgtcgcggcggaatccagtacaggtacgtggactacagtttggaccgaccttctgacagatatggactattataaaggccgcgcatatcgcattgaagacgttcccggggatgatgcggcattttatgcttttatcgcataccctattgatctgtttgaagaggggagcgtagtgaatgtgttcacctcattggtgggtaacgtcttcggatttaaggcggttcgcggtcttcgcttggaagatgtccgctttccattggcgtacgtaaagacttgcggggggccgccgcacgggatccaagtcgaacgtgataaaatgaacaaatacgggcgtccattattaggttgtacgatcaaaccgaaactgggtctgtcggccaaaaattacggccgcgctgtgtatgaatgcttgcgcggaggcctggatttcactaaagatgacgagaatatcaacagccagccgttcatgcgctggcgcgatcgcttcctgtttgtccaagacgccacagaaacagccgaagcccagaccggggaacgcaaagggcattatcttaacgttacggcacctactccggaagagatgtacaaacgcgccgaatttgcgaaagagatcggtgctcctatcattatgcacgattacatcacaggcggattcactgccaacacgggcctggcgaaatggtgtcaggacaacggtgtcctgcttcacatccaccgggcaatgcatgctgtcatcgatcgcaatccgaaccacggtattcattttcgtgtgctgacgaagattcttcgcctgtccggaggagatcacctgcataccgggactgtggttggcaaattagagggcgatcgtgctagcacactgggatggattgacctgctgcgggagagcttcattccagaagaccgctctcgtggcatttttttcgaccaagactggggtagcatgccaggagttttcgcggtggcgtcgggtgggatccatgtgtggcacatgccagcgctggtgaatatttttggagatgacagcgtcttgcaattcggaggcggtacgctggggcatccgtggggaaacgccgcgggtgcagcagccaaccgggtcgcattagaagcatgcgtcgaagcacgcaatcagggtcgcgatattgaaaaagagggcaaagaaatcctcacggcagcagcacagcattctccggaactgaagatcgcaatggagacatggaaggaaattaaattcgagtttgataccgtggataagttggacacacaaaaccgttaatactagagaaagaggagaaatactagatgagcaagccagatcgtgttgttctgatcggcgttgccggtgactccggttgcggcaagtcaaccttcctaaaccgccttgccgacctgtttggtaccgaactgatgaccgttatctgcctggatgactatcactctctggatcgcaagggccgtaaggaagcaggcgtaaccgctctggatccgcgcgccaacaactttgacctgatgtatgaacaggttaaggcgctgaagaacggcgaaaccatcatgaagccgatctacaaccatgaaaccggcctgatcgatccgccggaaaaaatcgaaccgaaccgcatcattgtgatcgagggtctgcatccgctttacgacgagcgcgtgcgtgaactgctggatttcagcgtttacctggacatcgatgacgaagttaaaatcgcttggaagatccaacgcgatatggcagaacgcggccactcctacgaagatgttctggcctctatcgaagcgcgccgcccggacttcaaggcctacattgagccgcagcgtggccatgcggacatcgttatccgcgttatgccgacccagctaatcccgaacgacaccgagcgcaaggtgctgcgtgtgcagctgatccaacgtgaaggccgcgatggttttgagccggcttacctgttcgacgaaggttctaccatccagtggaccccgtgcggtcgtaagctgacatgctcctatccgggcattcgcttagcctacggcccggacacctactacggtcacgaagtttcagtgcttgaggttgacggtcagttcgagaacctggaagagatgatctacgttgagggccacctgagcaagaccgacacccagtactacggtgagctgacccacctgctgctgcaacacaaagattacccgggttctaacaacggcaccggtctgttccaagtgctgaccggcctgaaaatgcgtgcggcctatgagcgtctgacctcccaagcagcaccggttgccgctagcgtttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z