LOCUS       BBa_K1465222            2371 bp    DNA     linear       26-Dec-2024
DEFINITION  Carboxysome (shell-proteins (csoS14)) with translational GFP 
            fusion (under T7 and without csoS2)
ACCESSION   BBa_K1465222
VERSION     BBa_K1465222.1  
FEATURES             Location/Qualifiers
     RBS             1..5
                     /label=Interspace region containing the natural RBS
     CDS             6..248
                     /label=csoS4A
     misc_feature    249..256
                     /label=Interspace region containing the natural RBS
     CDS             257..493
                     /label=csoS4B
     RBS             494..544
                     /label=Interspace region containing the natural RBS
     CDS             545..841
                     /label=csoS1C
     RBS             842..915
                     /label=Interspace region containing the natural RBS
     CDS             916..1209
                     /label=csoS1A
     misc_feature    1210..1926
                     /label=GFP (BBa_E0040)
     misc_feature    1927..1929
                     /label=Stopcodon
     RBS             1930..2038
                     /label=Interspace region containing the natural RBS
     CDS             2039..2371
                     /label=csoS1B
ORIGIN
        1 aaatcatgca agttgagaaa acgttggttt caacaaaccg tattgctgat atgggtcaca
       61 aaccactatt agtggtatgg gagaaaccgg gcgcgcccag gcaggtcgcc gtggatgcga
      121 ttggctgcat accgggcgat tgggttttgt gcgttgggtc atcggcagca cgagaggctg
      181 ccggaagcaa gtcttatccc tctgatttga cgattatcgg gattattgat cagtggaatg
      241 gtgagtaatg gaagtaatgc gcgttcgttc cgacctaatc gcaacacgca ggattcccgg
      301 tcttaaaaat atctctttgc gtgtcatgga ggatgctacg ggtaaggtca gtgtcgcttg
      361 cgatcccatt ggcgttcccg agggatgttg ggtctttacg attagcggct ctgccgctcg
      421 gtttggcgtg ggtgattttg agattctcac ggatttgacg attggtggca tcatcgatca
      481 ctgggtaact tgaccatcgc tagatgagtt gattttgaat gagtctttat tgaggagaga
      541 agaaatggca gcagtaacag gtattgcact gggtatgatt gaaacacgtg gtctggttcc
      601 agcgattgaa gctgccgatg ccatgaccaa ggccgccgaa gtacgtttgg ttggccgtca
      661 atttgttggt ggtggttacg tgaccgtttt ggtccgtggt gaaaccggtg ccgtcaacgc
      721 agcagttcgt gcgggcgctg atgcctgcga acgagtcggc gatggtctgg tcgcggcgca
      781 tatcattgcc cgtgtccatt ccgaagtcga aaacatcctg ccgaaagccc ctgaagctta
      841 aggattggga aagacgaacc ggcgcaggct tgttccggtt ctttgcataa agtgacagct
      901 taggagttta tttaaatggc tgatgtaact ggtattgctc tgggtatgat cgaaacacgt
      961 ggcttagttc ctgcgattga agcagcggac gccatgacta aagcggctga agtgcgtttg
     1021 gtcggtcgtc aatttgttgg tggcggttac gtcaccgtat tggttcgggg cgaaacaggc
     1081 gctgttaacg ccgctgttcg tgctggcgcc gatgcttgcg aacgtgttgg tgatggcttg
     1141 gttgctgcgc acatcattgc gcgtgtccac tcagaagtag aaaacattct gcctaaggcg
     1201 ccacaagcca tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     1261 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     1321 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     1381 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
     1441 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     1501 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     1561 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     1621 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     1681 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     1741 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1801 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1861 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     1921 aaataataag tcagatattc ctaagacggc tcacttcggc gacacccgtc gccgaaaccg
     1981 tctaaccaaa tttctgggct cgttctgaac gtgctcacct agttttagag gatctgttat
     2041 ggcaacgact cacggtattg ccctgggcat gattgaaaca cgaggattgg ttcctgccat
     2101 tgaagccgca gatgccatga ccaaagcggc ggaagtccgt ctggtcggac gatcatttgt
     2161 tggcggcggt tacgtgaccg taatggttcg tggtgagaca ggtgcagtaa atgctgccgt
     2221 tcgtgcgggt gctgacgcct gtgaacgtgt tggcgatggc ctggttgctg cgcacatcat
     2281 tgcgcgcgtt cattctgaag ttgagatcat cctacccgag acgcccgaag actcagattc
     2341 cgcgtggtgt atcgcaaatc tgaatagcta a
//