LOCUS BBa_K1465223 5084 bp DNA linear 22-Nov-2024 DEFINITION Functional carboxysome of Halothiobacillus neapolitanus with a GFP fusion under T7 control ACCESSION BBa_K1465223 VERSION BBa_K1465223.1 FEATURES Location/Qualifiers promoter 1..23 /label=BBa_I719005 RBS 32..83 /label=Interspace region containing the natural RBS CDS 84..2705 /label=shell associated protein csoS2 misc_feature 32..2705 /label=BBa_K1465204 RBS 2714..2718 /label=Interspace region containing the natural RBS CDS 2719..2961 /label=csoS4A misc_feature 2962..2969 /label=Interspace region containing the natural RBS CDS 2970..3206 /label=csoS4B RBS 3207..3257 /label=Interspace region containing the natural RBS CDS 3258..3554 /label=csoS1C RBS 3555..3628 /label=Interspace region containing the natural RBS CDS 3629..3922 /label=csoS1A misc_feature 3923..4639 /label=GFP (BBa_E0040) misc_feature 4640..4642 /label=Stopcodon RBS 4643..4751 /label=Interspace region containing the natural RBS CDS 4752..5084 /label=csoS1B misc_feature 2714..5084 /label=BBa_K1465222 ORIGIN 1 taatacgact cactataggg agatactaga ggtcaagtct gtcattgcgc tgtgcgtttc 61 tctacgcaca gcacttattc aagatggggt caaacatgcc ttcacagtca ggaatgaatc 121 ctgccgacct gagcggactc tctggcaagg aactggcacg cgcacgccgc gctgcactat 181 ccaagcaagg gaaagcagct gtttctaata aaacggctag cgtaaaccgt agcactaaac 241 aggcggcatc ttcgatcaat acaaatcagg tgcgctcttc tgtaaatgaa gtgcccactg 301 attaccaaat ggcggatcaa ttgtgctcta cgattgatca tgctgacttt ggtaccgaaa 361 gcaatcgcgt tagagatctc tgccgtcaac gcagagaggc actatcaact atcggtaaaa 421 aagcggttaa aaccaacggc aagccgtcgg gtcgcgttcg accacagcaa tcagtggttc 481 acaacgacgc aatgatcgaa aatgccggtg atactaacca atcatcgtcc acttcattaa 541 ataatgaact ttccgaaatc tgctccatag cagacgacat gccggagcgt tttggttcac 601 aagccaaaac cgtccgtgat atctgccgtg cacgccgtca agcgctctct gagcgtggaa 661 ctcgcgccgt gccgccaaag ccgcaatctc aaggtggtcc aggacgcaat ggctatcaaa 721 ttgatggata cctagatacc gcacttcatg gccgcgatgc cgccaagcgc caccgtgaaa 781 tgctctgtca atacggccgc ggcacagcac cttcctgcaa gccaacaggc cgtgtcaaaa 841 attctgtaca gtcgggcaac gcagcgccaa aaaaggttga aaccggtcac accctatccg 901 gcggatctgt tacgggcacg caagtggatc gtaaatctca tgtgactgga aacgagccgg 961 gcacttgccg agcagtcacg ggcaccgagt acgtaggtac tgagcaattc acctcttttt 1021 gcaataccag ccccaagcca aatgcgacga aggtcaatgt gaccacaacg gctcgtggtc 1081 gccctgttag cggtacggaa gtttcacgga ccgagaaggt aactggcaac gaatccggtg 1141 tctgccgtaa cgttaccggc accgaataca tgagtaatga agctcacttt tctctatgtg 1201 gaacagccgc aaagccttca caagcggata aagtcatgtt cggcgccaca gcacgaacgc 1261 atcaagtggt cagtggcagt gataaattca ggccctcttc tgttacgggt aacgaatcgg 1321 gtgcaaaacg cacaattacc ggctcgcagt acgcagacga aggtcttgcg cgactcacga 1381 tcaacggagc acctgcaaaa gtagccagaa cccacacctt tgcgggctct gacgttaccg 1441 gcacggaaat cggtcgctct actcgcgtaa ctggtgatga aagcggttcg tgtcgttcaa 1501 tctcaggcac cgagtatctc agtaacgagc aattccaatc cttttgtgac acaaaacctc 1561 aacgcagccc gttcaaggtt ggccaagatc gcacgaacaa gggtcagtct gtgactggta 1621 acttggttga tcgttccgaa ctggttacag gtaacgaacc aggttcatgc tctcgggtta 1681 caggctctca gtatggccaa agcaaaatct gcggtggtgg cgtgggaaaa gtgcgctcaa 1741 tgcgcaccct tcgcggcacc tcagtatctg gccaacagct agatcatgcc ccaaagatgt 1801 ccggtgacga gcgcggcggg tgcatgcccg tcaccggtaa tgagtactac ggtcgtgaac 1861 atttcgaacc gttttgtacg agcaccccag agcccgaagc tcaatcaact gaacaatcat 1921 tgacctgtga aggacaaatt attagcggca cttcagttga cgccagtgat ttggtcacag 1981 gaaatgaaat cggtgaacag caactcatca gcggtgacgc ctatgttggc gcgcagcaga 2041 caggttgcct tcccactaat ccacgcttca accaaactgg caatgttcag tcaatgggtt 2101 ttaagaacac caatcagcca gaacaaaact ttgcaccagg tgaagtaatg cctactgact 2161 ttagtattca aaccccagct cgctcggctc agaatcgcat tacaggtaac gacattgcgc 2221 cctcaggtcg cattacaggc cctggtatgc tggcaaccgg cttgattaca ggaacccgcg 2281 tattcaggca cgctgcgcgc gagttggttg gttctccaca acccatggca atggccatgg 2341 ccaaccgtaa taaggctgct caagcacctg ttgtgcagcc agaagtggtt gcaactcagg 2401 aaaagcctga gttggtatgt gcaccaagaa gcgatcaaat ggatcgtgtg agtggcgaag 2461 gcaaagaacg ttgccacatc actggcgatg actggtcagt taacaagcac atcaccggta 2521 cagccggtca atgggcgagt ggtcgcaacc cttccatgcg cggtaatgcg cgtgtggtcg 2581 aaaccagcgc gtttgccaat cgcaatgtgc caaaacctga aaagccgggc tccaagatca 2641 cgggcagtag tggtaatgac acccaaggta gtctgatcac ttactccggc ggcgcgcgcg 2701 gttaatacta gagaaatcat gcaagttgag aaaacgttgg tttcaacaaa ccgtattgct 2761 gatatgggtc acaaaccact attagtggta tgggagaaac cgggcgcgcc caggcaggtc 2821 gccgtggatg cgattggctg cataccgggc gattgggttt tgtgcgttgg gtcatcggca 2881 gcacgagagg ctgccggaag caagtcttat ccctctgatt tgacgattat cgggattatt 2941 gatcagtgga atggtgagta atggaagtaa tgcgcgttcg ttccgaccta atcgcaacac 3001 gcaggattcc cggtcttaaa aatatctctt tgcgtgtcat ggaggatgct acgggtaagg 3061 tcagtgtcgc ttgcgatccc attggcgttc ccgagggatg ttgggtcttt acgattagcg 3121 gctctgccgc tcggtttggc gtgggtgatt ttgagattct cacggatttg acgattggtg 3181 gcatcatcga tcactgggta acttgaccat cgctagatga gttgattttg aatgagtctt 3241 tattgaggag agaagaaatg gcagcagtaa caggtattgc actgggtatg attgaaacac 3301 gtggtctggt tccagcgatt gaagctgccg atgccatgac caaggccgcc gaagtacgtt 3361 tggttggccg tcaatttgtt ggtggtggtt acgtgaccgt tttggtccgt ggtgaaaccg 3421 gtgccgtcaa cgcagcagtt cgtgcgggcg ctgatgcctg cgaacgagtc ggcgatggtc 3481 tggtcgcggc gcatatcatt gcccgtgtcc attccgaagt cgaaaacatc ctgccgaaag 3541 cccctgaagc ttaaggattg ggaaagacga accggcgcag gcttgttccg gttctttgca 3601 taaagtgaca gcttaggagt ttatttaaat ggctgatgta actggtattg ctctgggtat 3661 gatcgaaaca cgtggcttag ttcctgcgat tgaagcagcg gacgccatga ctaaagcggc 3721 tgaagtgcgt ttggtcggtc gtcaatttgt tggtggcggt tacgtcaccg tattggttcg 3781 gggcgaaaca ggcgctgtta acgccgctgt tcgtgctggc gccgatgctt gcgaacgtgt 3841 tggtgatggc ttggttgctg cgcacatcat tgcgcgtgtc cactcagaag tagaaaacat 3901 tctgcctaag gcgccacaag ccatgcgtaa aggagaagaa cttttcactg gagttgtccc 3961 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg 4021 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact 4081 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag 4141 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt 4201 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa 4261 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga 4321 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat 4381 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga 4441 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt 4501 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga 4561 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat 4621 ggatgaacta tacaaataat aagtcagata ttcctaagac ggctcacttc ggcgacaccc 4681 gtcgccgaaa ccgtctaacc aaatttctgg gctcgttctg aacgtgctca cctagtttta 4741 gaggatctgt tatggcaacg actcacggta ttgccctggg catgattgaa acacgaggat 4801 tggttcctgc cattgaagcc gcagatgcca tgaccaaagc ggcggaagtc cgtctggtcg 4861 gacgatcatt tgttggcggc ggttacgtga ccgtaatggt tcgtggtgag acaggtgcag 4921 taaatgctgc cgttcgtgcg ggtgctgacg cctgtgaacg tgttggcgat ggcctggttg 4981 ctgcgcacat cattgcgcgc gttcattctg aagttgagat catcctaccc gagacgcccg 5041 aagactcaga ttccgcgtgg tgtatcgcaa atctgaatag ctaa //