LOCUS BBa_K1465226 1490 bp DNA linear 25-Nov-2024 DEFINITION sRNA of phosphofructokinase A under the control of ptac promoter (ptac_sRNA:pfkA) ACCESSION BBa_K1465226 VERSION BBa_K1465226.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=stop CDS 1..1084 /label=LacI misc_feature 1081..1084 /label=start RBS 1085..1092 /label=rbs promoter 1093..1153 /label=LacIq promoter misc_feature 1..1153 /label=BBa_K731300 misc_feature 1194..1219 /label=lacO operator promoter 1162..1188 /label=Ptac promoter promoter 1..1239 /label=BBa_K731500 misc_feature 1248..1271 /label=regulatory sRNA for the pfkA misc_feature 1272..1356 /label=Scaffold originated from MicC stem_loop 1357..1490 /label=Transcriptional terminator (T1/TE) misc_feature 1248..1490 /label=BBa_K1465225 ORIGIN 1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga 121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc 181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata 241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag 301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat 361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc 421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg 481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat 541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga 601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt 661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag 721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct 781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc 841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa 901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat 961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg 1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt 1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt 1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt 1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagtac taattctttt 1261 agccacacaa ctttctgttg ggccattgca ttgccactga ttttccaaca tataaaaaga 1321 caagcccgaa cagtcgtccg ggcttttttt ctcgagctcg agccaggcat caaataaaac 1381 gaaaggctca gtcgaaagac tgggcctttc gttttatctg tttttgtcgg tgaacgctct 1441 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //