LOCUS       BBa_K1465229            2222 bp    DNA     linear       01-Jun-2024
DEFINITION  Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus 
            under the control of ptac
ACCESSION   BBa_K1465229
VERSION     BBa_K1465229.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=stop
     CDS             1..1084
                     /label=LacI
     misc_feature    1081..1084
                     /label=start
     RBS             1085..1092
                     /label=rbs
     promoter        1093..1153
                     /label=LacIq promoter
     misc_feature    1..1153
                     /label=BBa_K731300
     misc_feature    1194..1219
                     /label=lacO operator
     promoter        1162..1188
                     /label=Ptac promoter
     promoter        1..1239
                     /label=BBa_K731500
     RBS             1248..1259
                     /label=B0034
     CDS             1260..2222
                     /label=Sedoheptulose 1,7-bisphosphatase (glpX)
     misc_feature    1248..2222
                     /label=BBa_K1465228
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt
     1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagaaa gaggagaaaa
     1261 tgagggaatt gaaaagcgaa aagcgtgttc agtcgttagc tatggaattt ctctctgtag
     1321 cacagcaagc agctctcgct tcttatcctt ggataggaaa aggtaataaa aacgaagttg
     1381 atagggctgg tacggaagct atgcgcaatc gactgaacct cattgatatg agcggtttaa
     1441 ttgttattgg tgaaggggaa atggacgaag ctcctatgct ttatattgga gaggaactcg
     1501 gaacaggaaa aggaccccaa ctcgatattg cagtagaccc tgttgatgga acgggtttaa
     1561 tggcaaaagg aatggataat tcaatagcag taattgctgc atccactaga ggaagtttac
     1621 tgcatgcccc agatatgtac atggaaaaga tagctgtggg accaaaagca aaaggctgcg
     1681 taaatttgga cgcatcttta acagaaaata tgaaatcagt tgctaaagct ttagggaaag
     1741 atttaagaga attaactgta atgatacagg atagaccacg tcatgatcat ttgatccaac
     1801 aagtaagaga tgtaggggct agactcaaat tattttctga tggtgacgtt acaagggcaa
     1861 taggtactgc actcgaagaa gtagacgttg atatattagt aggaactggc ggtgctccag
     1921 aaggagtaat tgctgcaacc gcactgaagt gtttgggggg agatttccaa ggaagacttg
     1981 ctcctcaaaa cgaagaagaa tttgatcgct gtattacgat gggaataaca gatccaagaa
     2041 aaattttcac aatagatgaa attgtaaaat cagatgattg cttttttgta gcaacaggaa
     2101 taactgacgg actgcttata aatggtattc gaaaaaaaga agatggttta atgcaaacgc
     2161 actcttttct tacaattgga ggaagcagcg taaaatacca atttattgaa gcttatcatt
     2221 ga
//