LOCUS BBa_K1465302 6738 bp DNA linear 16-Jun-2024 DEFINITION Isobutanol pathway ACCESSION BBa_K1465302 VERSION BBa_K1465302.1 FEATURES Location/Qualifiers RBS 1..12 /label=RBS CDS 13..1728 /label=alsS RBS 1729..1740 /label=RBS CDS 1741..3216 /label=ilvC CDS 3229..5079 /label=ilvD CDS 5092..6738 /label=kivD RBS 5080..5091 /label=RBS RBS 3217..3228 /label=RBS ORIGIN 1 aaagaggaga aaatgttgac aaaagcaaca aaagaacaaa aatcccttgt gaaaaacaga 61 ggggcggagc ttgttgttga ttgcttagtg gagcaaggtg tcacacatgt atttggcatt 121 ccaggtgcaa aaattgatgc ggtatttgac gctttacaag ataaaggacc tgaaattatc 181 gttgcccggc acgaacaaaa cgcagcattc atggcccaag cagtcggccg tttaactgga 241 aaaccgggag tcgtgttagt cacatcagga ccgggtgcct ctaacttggc aacaggcctg 301 ctgacagcga acactgaagg agaccctgtc gttgcgcttg ctggaaacgt gatccgtgca 361 gatcgtttaa aacggacaca tcaatctttg gataatgcgg cgctattcca gccgattaca 421 aaatacagtg tagaagttca agatgtaaaa aatataccgg aagctgttac aaatgcattt 481 aggatagcgt cagcagggca ggctggggcc gcttttgtga gctttccgca agatgttgtg 541 aatgaagtca caaatacgaa aaacgtgcgt gctgttgcag cgccaaaact cggtccagca 601 gcagatgatg caatcagtgc ggccatagca aaaatccaaa cagcaaaact tcctgtcgtt 661 ttggtcggca tgaaaggcgg aagaccggaa gcaattaagg cggttcgcaa gcttttgaaa 721 aaggttcagc ttccatttgt tgaaacatat caagctgccg gtaccctttc cagagattta 781 gaggatcaat attttggccg tatcggtttg ttccgcaacc agcctggcga tttactgcta 841 gagcaggcag atgttgttct gacgatcggc tatgacccga ttgaatatga tccgaaattc 901 tggaatatca atggagaccg gacaattatc catttagacg agattatcgc tgacattgat 961 catgcttacc agcctgatct tgaattgatc ggtgacattc cgtccacgat caatcatatc 1021 gaacacgatg ctgtgaaagt ggaatttgca gagcgtgagc agaaaatcct ttctgattta 1081 aaacaatata tgcatgaagg tgagcaggtg ccagcagatt ggaaatcaga cagagcgcac 1141 cctcttgaaa tcgttaaaga gttgcgtaat gcagtcgatg atcatgttac agtaacttgc 1201 gatatcggtt cgcacgccat ttggatgtca cgttatttcc gcagctacga gccgttaaca 1261 ttaatgatca gtaacggtat gcaaacactc ggcgttgcgc ttccttgggc aatcggcgct 1321 tcattggtga aaccgggaga aaaagtggtt tctgtctctg gtgacggcgg tttcttattc 1381 tcagcaatgg aattagagac agcagttcga ctaaaagcac caattgtaca cattgtatgg 1441 aacgacagca catatgacat ggttgcattc cagcaattga aaaaatataa ccgtacatct 1501 gcggtcgatt tcggaaatat cgatatcgtg aaatatgcgg aaagcttcgg agcaactggc 1561 ttgcgcgtag aatcaccaga ccagctggca gatgttctgc gtcaaggcat gaacgctgaa 1621 ggtcctgtca tcatcgatgt cccggttgac tacagtgata acattaattt agcaagtgac 1681 aagcttccga aagaatttgg ggaactcatg aaaacggaaa ctgtctaaaa agaggagaaa 1741 atggctaact acttcaatac actgaatctg cgccagcagc tggcacagct gggcaaatgt 1801 cgctttatgg gccgcgatga atttgccgat ggcgcgagct accttcaggg taaaaaagta 1861 gtcatcgtcg gctgtggcgc acagggtctg aaccagggcc tgaacatgcg tgattctggt 1921 ctcgatatct cctacgctct gcgtaaagaa gcgattgccg agaagcgcgc gtcctggcgt 1981 aaagcgaccg aaaatggttt taaagtgggt acttacgaag aactgatccc acaggcggat 2041 ctggtgatta acctgacgcc ggacaagcag cactctgatg tagtgcgcac cgtacagcca 2101 ctgatgaaag acggcgcggc gctgggctac tcgcacggtt tcaacatcgt cgaagtgggc 2161 gagcagatcc gtaaagatat caccgtagtg atggttgcgc cgaaatgccc aggcaccgaa 2221 gtgcgtgaag agtacaaacg tgggttcggc gtaccgacgc tgattgccgt tcacccggaa 2281 aacgatccga aaggcgaagg catggcgatt gccaaagcct gggcggctgc aaccggtggt 2341 caccgtgcgg gtgtgctgga atcgtccttc gttgcggaag tgaaatctga tctgatgggc 2401 gagcaaacca tcctgtgcgg tatgttgcag gctggctctc tgctgtgctt cgacaagctg 2461 gtggaagaag gtaccgatcc agcatacgca gaaaaactga ttcagttcgg ttgggaaacc 2521 atcaccgaag cactgaaaca gggcggcatc accctgatga tggaccgtct ctctaacccg 2581 gcgaaactgc gtgcttatgc gctttctgaa cagctgaaag agatcatggc acccctgttc 2641 cagaaacata tggacgacat catctccggc gaattttctt ccggtatgat ggcggactgg 2701 gccaacgatg ataagaaact gctgacctgg cgtgaagaga ccggcaaaac cgcgtttgaa 2761 accgcgccgc agtatgaagg caaaatcggc gagcaggagt acttcgataa aggcgtactg 2821 atgattgcga tggtgaaagc gggcgttgaa ctggcgttcg aaaccatggt cgattccggc 2881 atcattgaag agtctgcata ttatgaatca ctgcacgagc tgccgctgat tgccaacacc 2941 atcgcccgta agcgtctgta cgaaatgaac gtggttatct ctgataccgc tgagtacggt 3001 aactatctgt tctcttacgc ttgtgtgccg ttgctgaaac cgtttatggc agagctgcaa 3061 ccgggcgacc tgggtaaagc tattccggaa ggcgcggtag ataacgggca actgcgtgat 3121 gtgaacgaag cgattcgcag ccatgcgatt gagcaggtag gtaagaaact gcgcggctat 3181 atgacagata tgaaacgtat tgctgttgcg ggttaaaaag aggagaaaat gcctaagtac 3241 cgttccgcca ccaccactca tggtcgtaat atggcgggtg ctcgtgcgct gtggcgcgcc 3301 accggaatga ccgacgccga tttcggtaag ccgattatcg cggttgtgaa ctcgttcacc 3361 caatttgtac cgggtcacgt ccatctgcgc gatctcggta aactggtcgc cgaacaaatt 3421 gaagcggctg gcggcgttgc caaagagttc aacaccattg cggtggatga tgggattgcc 3481 atgggccacg gggggatgct ttattcactg ccatctcgcg aactgatcgc tgattccgtt 3541 gagtatatgg tcaacgccca ctgcgccgac gccatggtct gcatctctaa ctgcgacaaa 3601 atcaccccgg ggatgctgat ggcttccctg cgcctgaata ttccggtgat ctttgtttcc 3661 ggcggcccga tggaggccgg gaaaaccaaa ctttccgatc agatcatcaa gctcgatctg 3721 gtcgatgcga tgatccaggg cgcagacccg aaagtatctg actcccagag cgatcaggtt 3781 gaacgttccg cgtgtccgac ctgcggttcc tgctccggga tgtttaccgc taactcaatg 3841 aactgcctga ccgaagcgct gggcctgtcg cagccgggca acggctcgct gctggcaacc 3901 cacgccgacc gtaagcagct gttccttaat gctggtaaac gcattgttga attgaccaaa 3961 cgttattacg agcaaaacga cgaaagtgca ctgccgcgta atatcgccag taaggcggcg 4021 tttgaaaacg ccatgacgct ggatatcgcg atgggtggat cgactaacac cgtacttcac 4081 ctgctggcgg cggcgcagga agcggaaatc gacttcacca tgagtgatat cgataagctt 4141 tcccgcaagg ttccacagct gtgtaaagtt gcgccgagca cccagaaata ccatatggaa 4201 gatgttcacc gtgctggtgg tgttatcggt attctcggcg aactggatcg cgcggggtta 4261 ctgaaccgtg atgtgaaaaa cgtacttggc ctgacgttgc cgcaaacgct ggaacaatac 4321 gacgttatgc tgacccagga tgacgcggta aaaaatatgt tccgcgcagg tccagcaggc 4381 attcgtacca cacaggcatt ctcgcaagat tgccgttggg atacgctgga cgacgatcgc 4441 gccaatggct gtatccgctc gctggaacac gcctacagca aagacggcgg cctggcggtg 4501 ctctacggta actttgcgga aaacggctgc atcgtgaaaa cggcaggcgt cgatgacagc 4561 atcctcaaat tcaccggccc ggcgaaagtg tacgaaagcc aggacgatgc ggtagaagcg 4621 attctcggcg gtaaagttgt cgccggagat gtggtagtaa ttcgctatga aggcccgaaa 4681 ggcggtccgg ggatgcagga aatgctctac ccaaccagct tcctgaaatc aatgggtctc 4741 ggcaaagcct gtgcgctgat caccgacggt cgtttctctg gtggcacctc tggtctttcc 4801 atcggccacg tctcaccgga agcggcaagc ggcggcagca ttggcctgat tgaagatggt 4861 gacctgatcg ctatcgacat cccgaaccgt ggcattcagt tacaggtaag cgatgccgaa 4921 ctggcggcgc gtcgtgaagc gcaggacgct cgaggtgaca aagcctggac gccgaaaaat 4981 cgtgaacgtc aggtctcctt tgccctgcgt gcttatgcca gcctggcaac cagcgccgac 5041 aaaggcgcgg tgcgcgataa atcgaaactg gggggttaaa aagaggagaa aatgtataca 5101 gtaggagatt acctattaga ccgattacac gagttaggaa ttgaagaaat ttttggagtc 5161 cctggagact ataacttaca atttttagat caaattattt cccgcaagga tatgaaatgg 5221 gtcggaaatg ctaatgaatt aaatgcttca tatatggctg atggctatgc tcgtactaaa 5281 aaagctgccg catttcttac aacctttgga gtaggtgaat tgagtgcagt taatggatta 5341 gcaggaagtt acgccgaaaa tttaccagta gtagaaatag tgggatcacc tacatcaaaa 5401 gttcaaaatg aaggaaaatt tgttcatcat acgctggctg acggtgattt taaacacttt 5461 atgaaaatgc acgaacctgt tacagcagct cgaactttac tgacagcaga aaatgcaacc 5521 gttgaaattg accgagtact ttctgcacta ttaaaagaaa gaaaacctgt ctatatcaac 5581 ttaccagttg atgttgctgc tgcaaaagca gagaaaccct cactcccttt gaaaaaagaa 5641 aactcaactt caaatacaag tgaccaagag atcttgaaca aaattcaaga aagcttgaaa 5701 aatgccaaaa aaccaatcgt gattacagga catgaaataa ttagttttgg cttagaaaaa 5761 acagtctctc aatttatttc aaagacaaaa ctacctatta cgacattaaa ctttggaaaa 5821 agttcagttg atgaagctct cccttcattt ttaggaatct ataatggtaa actctcagag 5881 cctaatctta aagtattcgt ggaatcagcc gacttcatcc tgatgcttgg agttaaactc 5941 acagactctt caacaggagc cttcactcat catttaaatg aaaataaaat gatttcactg 6001 aatatagatg aaggaaaaat atttaacgaa agcatccaaa attttgattt tgaatccctc 6061 atctcctctc tcttagacct aagcgaaata gaatacaaag gaaaatatat cgataaaaag 6121 caagaagact ttgttccatc aaatgcgctt ttatcacaag accgcctatg gcaagcagtt 6181 gaaaacctaa ctcaaagcaa tgaaacaatc gttgctgaac aagggacatc attctttggc 6241 gcttcatcaa ttttcttaaa accaaagagt cattttattg gtcaaccctt atggggatca 6301 attggatata cattcccagc agcattagga agccaaattg cagataaaga aagcagacac 6361 cttttattta ttggtgatgg ttcacttcaa cttacggtgc aagaattagg attagcaatc 6421 agagaaaaaa ttaatccaat ttgctttatt atcaataatg atggttatac agtcgaaaga 6481 gaaattcatg gaccaaatca aagctacaat gatattccaa tgtggaatta ctcaaaatta 6541 ccagaatcat ttggagcaac agaagaacga gtagtctcga aaatcgttag aactgaaaat 6601 gaatttgtgt ctgtcatgaa agaagctcaa gcagatccaa atagaatgta ctggattgag 6661 ttaattttgg caaaagaaga tgcaccaaaa gtactgaaaa aaatgggcaa actatttgct 6721 gaacaaaata aatcataa //