##gff-version 3
##sequence-region BBa_K1465306 1 7985
BBa_K1465306	.	engineered_region	1248	7985	.	+	0	ID=BBa_K1465302;Name=BBa_K1465302
BBa_K1465306	.	CDS	2988	4463	.	+	0	ID=annotation2404135;Name=ilvC;Parent=BBa_K1465302
BBa_K1465306	.	CDS	1260	2975	.	+	0	ID=annotation2404132;Name=alsS;Parent=BBa_K1465302
BBa_K1465306	.	CDS	4476	6326	.	+	0	ID=annotation2404137;Name=ilvD;Parent=BBa_K1465302
BBa_K1465306	.	ribosome_entry_site	6327	6338	.	+	0	ID=annotation2404139;Name=RBS;Parent=BBa_K1465302
BBa_K1465306	.	ribosome_entry_site	4464	4475	.	+	0	ID=annotation2404140;Name=RBS;Parent=BBa_K1465302
BBa_K1465306	.	ribosome_entry_site	2976	2987	.	+	0	ID=annotation2404133;Name=RBS;Parent=BBa_K1465302
BBa_K1465306	.	CDS	6339	7985	.	+	0	ID=annotation2404138;Name=kivD;Parent=BBa_K1465302
BBa_K1465306	.	ribosome_entry_site	1248	1259	.	+	0	ID=annotation2404130;Name=RBS;Parent=BBa_K1465302
BBa_K1465306	.	promoter	1	1239	.	+	0	ID=BBa_K731500;Name=BBa_K731500
BBa_K1465306	.	operator	1194	1219	.	+	0	ID=annotation2181545;Name=lacO operator;Parent=BBa_K731500
BBa_K1465306	.	stop_codon	1	3	.	+	0	ID=annotation2181539;Name=stop;Parent=BBa_K731500
BBa_K1465306	.	engineered_region	1	1153	.	+	0	ID=annotation2181544;Name=BBa_K731300;Parent=BBa_K731500
BBa_K1465306	.	start_codon	1081	1084	.	+	0	ID=annotation2181541;Name=start;Parent=BBa_K731500
BBa_K1465306	.	promoter	1093	1153	.	+	0	ID=annotation2181543;Name=LacIq promoter;Parent=BBa_K731500
BBa_K1465306	.	promoter	1162	1188	.	+	0	ID=annotation2181546;Name=Ptac promoter;Parent=BBa_K731500
BBa_K1465306	.	CDS	1	1084	.	+	0	ID=annotation2181540;Name=LacI;Parent=BBa_K731500
BBa_K1465306	.	ribosome_entry_site	1085	1092	.	+	0	ID=annotation2181542;Name=rbs;Parent=BBa_K731500
>BBa_K1465306
tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtt
tgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccct
gagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggat
ataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatg
gcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgca
tggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagata
tttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgaccc
aatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcag
agacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagtt
aatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctacc
atcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcaggg
ccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaatt
cagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacg
gtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactct
cttccgggcgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcatcggctcgta
taatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgttttactagagaaagaggagaaaatgtt
gacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaa
ggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaa
ttatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgt
gttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgtt
gcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagc
cgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtc
agcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgt
gctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaac
ttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttca
gcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggt
ttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaat
atgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgc
ttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaa
tttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattgga
aatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcga
tatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatg
caaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctg
gtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatg
gaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaat
atcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttc
tgcgtcaaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaag
tgacaagcttccgaaagaatttggggaactcatgaaaacggaaactgtctaaaaagaggagaaaatggctaactacttc
aatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaatttgccgatggcg
cgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcctgaacatgcgtga
ttctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgtaaagcgaccgaa
aatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgccggacaagcagc
actctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacggtttcaacatcgt
cgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcaccgaagtgcgtgaa
gagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcgaaggcatggcga
ttgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgcggaagtgaaatc
tgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgacaagctggtggaa
gaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcactgaaacagggcg
gcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaacagctgaaagagat
catggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatggcggactgggcc
aacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgcagtatgaaggca
aaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttgaactggcgttcga
aaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctgattgccaacacc
atcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatctgttctcttacg
cttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggt
agataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggc
tatatgacagatatgaaacgtattgctgttgcgggttaaaaagaggagaaaatgcctaagtaccgttccgccaccacca
ctcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgat
tatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaa
caaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccatgggccacgggg
ggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgc
catggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaatattccggtgatc
tttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcga
tgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttc
ctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcg
ctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttatt
acgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatat
cgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagt
gatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatg
ttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgt
acttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttc
cgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctggacgacgatcgcg
ccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggtaactttgcgga
aaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagc
caggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccga
aaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgct
gatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggc
agcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatg
ccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggt
ctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactgggg
ggttaaaaagaggagaaaatgtatacagtaggagattacctattagaccgattacacgagttaggaattgaagaaattt
ttggagtccctggagactataacttacaatttttagatcaaattatttcccgcaaggatatgaaatgggtcggaaatgc
taatgaattaaatgcttcatatatggctgatggctatgctcgtactaaaaaagctgccgcatttcttacaacctttgga
gtaggtgaattgagtgcagttaatggattagcaggaagttacgccgaaaatttaccagtagtagaaatagtgggatcac
ctacatcaaaagttcaaaatgaaggaaaatttgttcatcatacgctggctgacggtgattttaaacactttatgaaaat
gcacgaacctgttacagcagctcgaactttactgacagcagaaaatgcaaccgttgaaattgaccgagtactttctgca
ctattaaaagaaagaaaacctgtctatatcaacttaccagttgatgttgctgctgcaaaagcagagaaaccctcactcc
ctttgaaaaaagaaaactcaacttcaaatacaagtgaccaagagatcttgaacaaaattcaagaaagcttgaaaaatgc
caaaaaaccaatcgtgattacaggacatgaaataattagttttggcttagaaaaaacagtctctcaatttatttcaaag
acaaaactacctattacgacattaaactttggaaaaagttcagttgatgaagctctcccttcatttttaggaatctata
atggtaaactctcagagcctaatcttaaagtattcgtggaatcagccgacttcatcctgatgcttggagttaaactcac
agactcttcaacaggagccttcactcatcatttaaatgaaaataaaatgatttcactgaatatagatgaaggaaaaata
tttaacgaaagcatccaaaattttgattttgaatccctcatctcctctctcttagacctaagcgaaatagaatacaaag
gaaaatatatcgataaaaagcaagaagactttgttccatcaaatgcgcttttatcacaagaccgcctatggcaagcagt
tgaaaacctaactcaaagcaatgaaacaatcgttgctgaacaagggacatcattctttggcgcttcatcaattttctta
aaaccaaagagtcattttattggtcaacccttatggggatcaattggatatacattcccagcagcattaggaagccaaa
ttgcagataaagaaagcagacaccttttatttattggtgatggttcacttcaacttacggtgcaagaattaggattagc
aatcagagaaaaaattaatccaatttgctttattatcaataatgatggttatacagtcgaaagagaaattcatggacca
aatcaaagctacaatgatattccaatgtggaattactcaaaattaccagaatcatttggagcaacagaagaacgagtag
tctcgaaaatcgttagaactgaaaatgaatttgtgtctgtcatgaaagaagctcaagcagatccaaatagaatgtactg
gattgagttaattttggcaaaagaagatgcaccaaaagtactgaaaaaaatgggcaaactatttgctgaacaaaataaa
tcataa