LOCUS BBa_K1465307 9028 bp DNA linear 22-Nov-2024 DEFINITION Isobutanol pathway with adhA from Lactococcus lactis under Ptac control ACCESSION BBa_K1465307 VERSION BBa_K1465307.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=stop CDS 1..1084 /label=LacI misc_feature 1081..1084 /label=start RBS 1085..1092 /label=rbs promoter 1093..1153 /label=LacIq promoter misc_feature 1..1153 /label=BBa_K731300 misc_feature 1194..1219 /label=lacO operator promoter 1162..1188 /label=Ptac promoter promoter 1..1239 /label=BBa_K731500 RBS 1248..1259 /label=RBS CDS 1260..2975 /label=alsS RBS 2976..2987 /label=RBS CDS 2988..4463 /label=ilvC CDS 4476..6326 /label=ilvD CDS 6339..7985 /label=kivD RBS 6327..6338 /label=RBS RBS 4464..4475 /label=RBS misc_feature 1248..7985 /label=BBa_K1465302 RBS 7994..8005 /label=RBS CDS 8006..9028 /label=adhA misc_feature 7994..9028 /label=BBa_K1465301 misc_feature 1248..9028 /label=BBa_K1465303 ORIGIN 1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga 121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc 181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata 241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag 301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat 361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc 421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg 481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat 541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga 601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt 661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag 721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct 781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc 841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa 901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat 961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg 1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt 1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt 1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt 1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagaaa gaggagaaaa 1261 tgttgacaaa agcaacaaaa gaacaaaaat cccttgtgaa aaacagaggg gcggagcttg 1321 ttgttgattg cttagtggag caaggtgtca cacatgtatt tggcattcca ggtgcaaaaa 1381 ttgatgcggt atttgacgct ttacaagata aaggacctga aattatcgtt gcccggcacg 1441 aacaaaacgc agcattcatg gcccaagcag tcggccgttt aactggaaaa ccgggagtcg 1501 tgttagtcac atcaggaccg ggtgcctcta acttggcaac aggcctgctg acagcgaaca 1561 ctgaaggaga ccctgtcgtt gcgcttgctg gaaacgtgat ccgtgcagat cgtttaaaac 1621 ggacacatca atctttggat aatgcggcgc tattccagcc gattacaaaa tacagtgtag 1681 aagttcaaga tgtaaaaaat ataccggaag ctgttacaaa tgcatttagg atagcgtcag 1741 cagggcaggc tggggccgct tttgtgagct ttccgcaaga tgttgtgaat gaagtcacaa 1801 atacgaaaaa cgtgcgtgct gttgcagcgc caaaactcgg tccagcagca gatgatgcaa 1861 tcagtgcggc catagcaaaa atccaaacag caaaacttcc tgtcgttttg gtcggcatga 1921 aaggcggaag accggaagca attaaggcgg ttcgcaagct tttgaaaaag gttcagcttc 1981 catttgttga aacatatcaa gctgccggta ccctttccag agatttagag gatcaatatt 2041 ttggccgtat cggtttgttc cgcaaccagc ctggcgattt actgctagag caggcagatg 2101 ttgttctgac gatcggctat gacccgattg aatatgatcc gaaattctgg aatatcaatg 2161 gagaccggac aattatccat ttagacgaga ttatcgctga cattgatcat gcttaccagc 2221 ctgatcttga attgatcggt gacattccgt ccacgatcaa tcatatcgaa cacgatgctg 2281 tgaaagtgga atttgcagag cgtgagcaga aaatcctttc tgatttaaaa caatatatgc 2341 atgaaggtga gcaggtgcca gcagattgga aatcagacag agcgcaccct cttgaaatcg 2401 ttaaagagtt gcgtaatgca gtcgatgatc atgttacagt aacttgcgat atcggttcgc 2461 acgccatttg gatgtcacgt tatttccgca gctacgagcc gttaacatta atgatcagta 2521 acggtatgca aacactcggc gttgcgcttc cttgggcaat cggcgcttca ttggtgaaac 2581 cgggagaaaa agtggtttct gtctctggtg acggcggttt cttattctca gcaatggaat 2641 tagagacagc agttcgacta aaagcaccaa ttgtacacat tgtatggaac gacagcacat 2701 atgacatggt tgcattccag caattgaaaa aatataaccg tacatctgcg gtcgatttcg 2761 gaaatatcga tatcgtgaaa tatgcggaaa gcttcggagc aactggcttg cgcgtagaat 2821 caccagacca gctggcagat gttctgcgtc aaggcatgaa cgctgaaggt cctgtcatca 2881 tcgatgtccc ggttgactac agtgataaca ttaatttagc aagtgacaag cttccgaaag 2941 aatttgggga actcatgaaa acggaaactg tctaaaaaga ggagaaaatg gctaactact 3001 tcaatacact gaatctgcgc cagcagctgg cacagctggg caaatgtcgc tttatgggcc 3061 gcgatgaatt tgccgatggc gcgagctacc ttcagggtaa aaaagtagtc atcgtcggct 3121 gtggcgcaca gggtctgaac cagggcctga acatgcgtga ttctggtctc gatatctcct 3181 acgctctgcg taaagaagcg attgccgaga agcgcgcgtc ctggcgtaaa gcgaccgaaa 3241 atggttttaa agtgggtact tacgaagaac tgatcccaca ggcggatctg gtgattaacc 3301 tgacgccgga caagcagcac tctgatgtag tgcgcaccgt acagccactg atgaaagacg 3361 gcgcggcgct gggctactcg cacggtttca acatcgtcga agtgggcgag cagatccgta 3421 aagatatcac cgtagtgatg gttgcgccga aatgcccagg caccgaagtg cgtgaagagt 3481 acaaacgtgg gttcggcgta ccgacgctga ttgccgttca cccggaaaac gatccgaaag 3541 gcgaaggcat ggcgattgcc aaagcctggg cggctgcaac cggtggtcac cgtgcgggtg 3601 tgctggaatc gtccttcgtt gcggaagtga aatctgatct gatgggcgag caaaccatcc 3661 tgtgcggtat gttgcaggct ggctctctgc tgtgcttcga caagctggtg gaagaaggta 3721 ccgatccagc atacgcagaa aaactgattc agttcggttg ggaaaccatc accgaagcac 3781 tgaaacaggg cggcatcacc ctgatgatgg accgtctctc taacccggcg aaactgcgtg 3841 cttatgcgct ttctgaacag ctgaaagaga tcatggcacc cctgttccag aaacatatgg 3901 acgacatcat ctccggcgaa ttttcttccg gtatgatggc ggactgggcc aacgatgata 3961 agaaactgct gacctggcgt gaagagaccg gcaaaaccgc gtttgaaacc gcgccgcagt 4021 atgaaggcaa aatcggcgag caggagtact tcgataaagg cgtactgatg attgcgatgg 4081 tgaaagcggg cgttgaactg gcgttcgaaa ccatggtcga ttccggcatc attgaagagt 4141 ctgcatatta tgaatcactg cacgagctgc cgctgattgc caacaccatc gcccgtaagc 4201 gtctgtacga aatgaacgtg gttatctctg ataccgctga gtacggtaac tatctgttct 4261 cttacgcttg tgtgccgttg ctgaaaccgt ttatggcaga gctgcaaccg ggcgacctgg 4321 gtaaagctat tccggaaggc gcggtagata acgggcaact gcgtgatgtg aacgaagcga 4381 ttcgcagcca tgcgattgag caggtaggta agaaactgcg cggctatatg acagatatga 4441 aacgtattgc tgttgcgggt taaaaagagg agaaaatgcc taagtaccgt tccgccacca 4501 ccactcatgg tcgtaatatg gcgggtgctc gtgcgctgtg gcgcgccacc ggaatgaccg 4561 acgccgattt cggtaagccg attatcgcgg ttgtgaactc gttcacccaa tttgtaccgg 4621 gtcacgtcca tctgcgcgat ctcggtaaac tggtcgccga acaaattgaa gcggctggcg 4681 gcgttgccaa agagttcaac accattgcgg tggatgatgg gattgccatg ggccacgggg 4741 ggatgcttta ttcactgcca tctcgcgaac tgatcgctga ttccgttgag tatatggtca 4801 acgcccactg cgccgacgcc atggtctgca tctctaactg cgacaaaatc accccgggga 4861 tgctgatggc ttccctgcgc ctgaatattc cggtgatctt tgtttccggc ggcccgatgg 4921 aggccgggaa aaccaaactt tccgatcaga tcatcaagct cgatctggtc gatgcgatga 4981 tccagggcgc agacccgaaa gtatctgact cccagagcga tcaggttgaa cgttccgcgt 5041 gtccgacctg cggttcctgc tccgggatgt ttaccgctaa ctcaatgaac tgcctgaccg 5101 aagcgctggg cctgtcgcag ccgggcaacg gctcgctgct ggcaacccac gccgaccgta 5161 agcagctgtt ccttaatgct ggtaaacgca ttgttgaatt gaccaaacgt tattacgagc 5221 aaaacgacga aagtgcactg ccgcgtaata tcgccagtaa ggcggcgttt gaaaacgcca 5281 tgacgctgga tatcgcgatg ggtggatcga ctaacaccgt acttcacctg ctggcggcgg 5341 cgcaggaagc ggaaatcgac ttcaccatga gtgatatcga taagctttcc cgcaaggttc 5401 cacagctgtg taaagttgcg ccgagcaccc agaaatacca tatggaagat gttcaccgtg 5461 ctggtggtgt tatcggtatt ctcggcgaac tggatcgcgc ggggttactg aaccgtgatg 5521 tgaaaaacgt acttggcctg acgttgccgc aaacgctgga acaatacgac gttatgctga 5581 cccaggatga cgcggtaaaa aatatgttcc gcgcaggtcc agcaggcatt cgtaccacac 5641 aggcattctc gcaagattgc cgttgggata cgctggacga cgatcgcgcc aatggctgta 5701 tccgctcgct ggaacacgcc tacagcaaag acggcggcct ggcggtgctc tacggtaact 5761 ttgcggaaaa cggctgcatc gtgaaaacgg caggcgtcga tgacagcatc ctcaaattca 5821 ccggcccggc gaaagtgtac gaaagccagg acgatgcggt agaagcgatt ctcggcggta 5881 aagttgtcgc cggagatgtg gtagtaattc gctatgaagg cccgaaaggc ggtccgggga 5941 tgcaggaaat gctctaccca accagcttcc tgaaatcaat gggtctcggc aaagcctgtg 6001 cgctgatcac cgacggtcgt ttctctggtg gcacctctgg tctttccatc ggccacgtct 6061 caccggaagc ggcaagcggc ggcagcattg gcctgattga agatggtgac ctgatcgcta 6121 tcgacatccc gaaccgtggc attcagttac aggtaagcga tgccgaactg gcggcgcgtc 6181 gtgaagcgca ggacgctcga ggtgacaaag cctggacgcc gaaaaatcgt gaacgtcagg 6241 tctcctttgc cctgcgtgct tatgccagcc tggcaaccag cgccgacaaa ggcgcggtgc 6301 gcgataaatc gaaactgggg ggttaaaaag aggagaaaat gtatacagta ggagattacc 6361 tattagaccg attacacgag ttaggaattg aagaaatttt tggagtccct ggagactata 6421 acttacaatt tttagatcaa attatttccc gcaaggatat gaaatgggtc ggaaatgcta 6481 atgaattaaa tgcttcatat atggctgatg gctatgctcg tactaaaaaa gctgccgcat 6541 ttcttacaac ctttggagta ggtgaattga gtgcagttaa tggattagca ggaagttacg 6601 ccgaaaattt accagtagta gaaatagtgg gatcacctac atcaaaagtt caaaatgaag 6661 gaaaatttgt tcatcatacg ctggctgacg gtgattttaa acactttatg aaaatgcacg 6721 aacctgttac agcagctcga actttactga cagcagaaaa tgcaaccgtt gaaattgacc 6781 gagtactttc tgcactatta aaagaaagaa aacctgtcta tatcaactta ccagttgatg 6841 ttgctgctgc aaaagcagag aaaccctcac tccctttgaa aaaagaaaac tcaacttcaa 6901 atacaagtga ccaagagatc ttgaacaaaa ttcaagaaag cttgaaaaat gccaaaaaac 6961 caatcgtgat tacaggacat gaaataatta gttttggctt agaaaaaaca gtctctcaat 7021 ttatttcaaa gacaaaacta cctattacga cattaaactt tggaaaaagt tcagttgatg 7081 aagctctccc ttcattttta ggaatctata atggtaaact ctcagagcct aatcttaaag 7141 tattcgtgga atcagccgac ttcatcctga tgcttggagt taaactcaca gactcttcaa 7201 caggagcctt cactcatcat ttaaatgaaa ataaaatgat ttcactgaat atagatgaag 7261 gaaaaatatt taacgaaagc atccaaaatt ttgattttga atccctcatc tcctctctct 7321 tagacctaag cgaaatagaa tacaaaggaa aatatatcga taaaaagcaa gaagactttg 7381 ttccatcaaa tgcgctttta tcacaagacc gcctatggca agcagttgaa aacctaactc 7441 aaagcaatga aacaatcgtt gctgaacaag ggacatcatt ctttggcgct tcatcaattt 7501 tcttaaaacc aaagagtcat tttattggtc aacccttatg gggatcaatt ggatatacat 7561 tcccagcagc attaggaagc caaattgcag ataaagaaag cagacacctt ttatttattg 7621 gtgatggttc acttcaactt acggtgcaag aattaggatt agcaatcaga gaaaaaatta 7681 atccaatttg ctttattatc aataatgatg gttatacagt cgaaagagaa attcatggac 7741 caaatcaaag ctacaatgat attccaatgt ggaattactc aaaattacca gaatcatttg 7801 gagcaacaga agaacgagta gtctcgaaaa tcgttagaac tgaaaatgaa tttgtgtctg 7861 tcatgaaaga agctcaagca gatccaaata gaatgtactg gattgagtta attttggcaa 7921 aagaagatgc accaaaagta ctgaaaaaaa tgggcaaact atttgctgaa caaaataaat 7981 cataatacta gagaaagagg agaaaatgaa agcagcagta gtaagacaca atccagatgg 8041 ttatgcggac cttgttgaaa aggaacttcg agcaatcaaa cctaatgaag ctttgcttga 8101 catggagtat tgtggagtct gtcataccga tttgcacgtt gcagcaggtg attatggcaa 8161 caaagcaggg actgttcttg gtcatgaagg aattggaatt gtcaaagaaa ttggagctga 8221 tgtaagctcg cttcaagttg gtgatcgggt ttcagtggct tggttctttg aaggatgtgg 8281 tcactgtgaa tactgtgtat ctggtaatga aactttttgt cgagaagtta aaaatgcagg 8341 atattcagtt gatggcggaa tggctgaaga agcaattgtt gttgccgatt atgctgtcaa 8401 agttcctgac ggacttgacc caattgaagc tagctcaatt acttgtgctg gagtaacaac 8461 ttacaaagca atcaaagtat caggagtaaa acctggtgat tggcaagtaa tttttggtgc 8521 tggaggactt ggaaatttag caattcaata tgctaaaaat gtttttggag caaaagtaat 8581 tgctgttgat attaatcaag ataaattaaa tttagctaaa aaaattggag ctgatgtgat 8641 tatcaattct ggtgatgtaa atccagttga tgaaattaaa aaaataactg gcggcttagg 8701 ggtgcaaagt gcaatagttt gtgctgttgc aaggattgct tttgaacaag cggttgcttc 8761 tttgaaacct atgggcaaaa tggttgctgt ggcacttccc aatactgaga tgactttatc 8821 agttccaaca gttgtttttg acggagtgga ggttgcaggt tcacttgtcg gaacaagact 8881 tgacttggca gaagcttttc aatttggagc agaaggtaag gtaaaaccaa ttgttgcgac 8941 acgcaaactg gaagaaatca atgatattat tgatgaaatg aaggcaggaa aaattgaagg 9001 ccgaatggtc attgatttta ctaaataa //