Isobutanol pathway with adhA from Lactococcus lactis under Ptac control
[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward
This is E.coli lactose-inducibile promoter. lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow.
Isobutanol pathway with adhA from Lactococcus lactis
adhA gene from Lactococcus lactis
Conversion of the iGEM parts registry to SBOL2.1