LOCUS BBa_K1465401 3163 bp DNA linear 31-Oct-2024 DEFINITION Antibiotic-free selection plasmid (pSB1C3_alr_RFP) ACCESSION BBa_K1465401 VERSION BBa_K1465401.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=BioBrick suffix stem_loop 22..93 /label=E. coli his operon terminator stem_loop 30..64 /label=Stem loop primer_bind 157..176 /label=Verification reverse (VR) primer binding site misc_feature 261..875 /label=rep (pMB1) misc_feature 276..276 /label=ORI stem_loop 1042..1147 /label=T0 terminator CDS 1149..2228 /label=alanine racemase (BBa_K1172901) RBS 2232..2243 /label=BBa_B0034 CDS 2253..2912 /label=Chloramphenicol resistance primer_bind 3026..3045 /label=Verification forward (VF2) primer binding site stem_loop 3102..3105 /label=end of terminator stem_loop 3115..3134 /label=Stem loop misc_feature 3144..3163 /label=BioBrick prefix ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt 61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct 121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga 301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1021 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag 1081 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc 1141 aagcgagctt aatccacgta tttcatcgcg acccttgaag tcaggcgcgt aataagttcg 1201 taagcgctta ctttcgtcat ttcagcgata cgttctacgg gcaaaccttc gccccataaa 1261 atgaccggat ccccggcttt gtcctgcgcc tgtggaccta agtctacgca gatcatatcc 1321 atcgccacgc gcccgacaat cggtacttcg cgaccgttca ccagcactgg cgtaccggac 1381 ggcgcggcgc gcggataacc atcgccatag cccatcgcga ctacgccaag acgggtatca 1441 cgttcgctta cccaggttcc accataacca acaggctctc cggctttatg ctcacgcacg 1501 gcaatcaggc tggaggttag tgacatcact ggctgacagc caaaatcggc accggtggag 1561 cgatcttcca gcggcgagac gccataaaga atgatgcccg ggcgcaccca gtcaaaatgc 1621 gactgtggcc acagcagaat gccacccgac gcggcaatgg aacgttgacc aggtttgcct 1681 tcgcaaaagg tattaaagat agcgagttgt ttctcggttg cgccacattt tggttcatcc 1741 gcgcgcgcaa aatggctgac gatattcacc ggctgacgaa cgtttttgca ctgggtcagg 1801 cgatgataaa acgcctcagc ctgttccggc cttacgccca gacggtgcat accggtatcg 1861 agtttcatcc agacggtaac cggctcgtcc aggctagcct cttccagcgc agccagctgt 1921 tcttcgttat gcacggcggt atgaaaatgt tgcgcagaaa tcgtcggcag atctctggca 1981 tcaaaaaagc cttcgagtaa cagtacaggt ttggtgattc cccccgcacg cagtcgcaga 2041 gcttcttcga gacgggctac gccaaaggcg tcagcatcgg ggagcgttcg cgcggtctca 2101 agaagaccgt gaccataagc gttcgctttc accaccgcaa ccattttact ggcaggggcc 2161 agttcacgaa gacgttgcag gttgtgtcgc agagcgcggc ggttaatcac aacagttgcc 2221 gcttgcattt gtttctcctc tttctagtaa aattacgccc cgccctgcca ctcatcgcag 2281 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga 2341 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg 2401 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc 2461 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc 2521 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg 2581 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa 2641 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc 2701 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta 2761 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta 2821 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca 2881 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc 2941 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct 3001 cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac 3061 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc 3121 tttcgctaag gatgatttct ggaattcgcg gccgcttcta gag //