BBa_K1467100 1 BBa_K1467100 RFP coding device - Golden Gate Module Flipper 2014-09-23T11:00:00Z 2015-05-08T01:10:38Z Part:BBa_J04450 The colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong. LacI sensitive CAP sensitive This part is a variation of Part:BBa_J04450 with additional flanking sequences recognised by the enzyme BsaI that allow standard GoldenGate Modular Cloning (MoClo) Promoter+5' untranslated leader (Pro+5U) modules to be 'flipped' in a one-pot, one-step, digestion-ligation reaction with BsaI, thus converting the MoClo part to a BioBrick part. When cloning is successful the colonies become white as the RFP sequence is replaced by the new part. false false _1846_ 0 20866 9 In stock true The sequences flanking the RFP part contain the recognition sequence for BsaI and the (separate) cut-site for BsaI. The cut sites produce 4base pair 5' overhangs compatible with GoldenGate Modular Cloning (MoClo) Promoter+5' untranslated leader (Pro+5U) modules. false Cara Deal, Jessica Gray BBa_K1467100_sequence 1 ggagtgagaccgcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcaaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z