LOCUS       BBa_K1468004            3129 bp    DNA     linear       22-Dec-2024
DEFINITION  pTet + gene encoding LacZ
ACCESSION   BBa_K1468004
VERSION     BBa_K1468004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     promoter        20..25
                     /label=-35
     misc_feature    1..54
                     /label=BBa_R0040
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     CDS             61..3129
                     /label=LacZ
     misc_feature    61..63
                     /label=START
     misc_feature    3085..3085
                     /label=C --> T
     misc_feature    3129..3129
                     /label=STOP
     misc_feature    61..3129
                     /label=BBa_I732005
     misc_feature    1..3129
                     /label=BBa_K1468004
     CDS             61..3129
                     /label=LacZ protein
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatgacc
       61 atgattacgg attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      121 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      181 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc
      241 tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc
      301 gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc
      361 aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt
      421 tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt
      481 atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac
      541 ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa
      601 aaccgcctcg cggtgatggt gctgcgctgg agtgacggca gttatctgga agatcaggat
      661 atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa
      721 atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag
      781 gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg
      841 cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag
      901 cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg
      961 agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg
     1021 ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg
     1081 ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct
     1141 ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag
     1201 aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg
     1261 tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg
     1321 ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg
     1381 cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa
     1441 tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc
     1501 cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg
     1561 atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa
     1621 aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg
     1681 atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt
     1741 ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac
     1801 ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc
     1861 tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa
     1921 caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa
     1981 tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag
     2041 ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa
     2101 ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg
     2161 caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt
     2221 ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc
     2281 accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag
     2341 tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc
     2401 gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt
     2461 gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg
     2521 ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg
     2581 tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt
     2641 ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg
     2701 attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg
     2761 ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca
     2821 ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg
     2881 cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc
     2941 tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc
     3001 acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg
     3061 tcagtatcgg cggaatttca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt
     3121 caaaaataa
//