BBa_K1470001
1
BBa_K1470001
puromycin N-acetyl-transferase
2014-09-30T11:00:00Z
2015-05-08T01:10:38Z
puromycin N-acetyl-transferase is a protein of Streptomyces alboniger. We got thi spart from iGEM Freiburg 2013
Puromycin is an antibodic derived from Streptomyces alboniger. The substance affects the cell both in protein translation and mitochondrial protein import. Due to this, it's toxic to many organisms such as bacteria, Trypanosoma and humans.
A small part of its structure resembles to aminoacetylated tRNA and is recognized by ribosomes. the nascent chain containing puromycin leads to a translational stop.
Streptomyces alboniger protects itself from puromycin with a acetyl transferase. It uses acetyl-CoA to bind the the acetyl group with the amino group. In this state puromycin cannot be recognized by the ribosom anymore.
This enzyme is a great tool for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis nearly over 99 % of unimfected cells will be dead within tows days.
false
false
_1849_
0
20909
9
It's complicated
false
We had no design consideration, because the sequence contains no restriction sites at all.
false
Pascal Sartor
BBa_K1470001_sequence
1
atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgcggccgtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggagtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z