LOCUS       BBa_K1471009            2623 bp    DNA     linear       14-Nov-2024
DEFINITION  RBS - PhyB - AGS - VP16 - NLS - PolyA.
ACCESSION   BBa_K1471009
VERSION     BBa_K1471009.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=Kozak Sequence.
     misc_feature    1..1
                     /label=Ribosome binds to 5' end.
     misc_feature    8..10
                     /label=Receptor codon.
     RBS             1..11
                     /label=BBa_K1471011
     misc_feature    18..1967
                     /label=PhyB
     CDS             18..1967
                     /label=BBa_K1150004
     misc_feature    1976..1984
                     /label=AGS Linker
     CDS             1976..1984
                     /label=BBa_K1150013
     misc_feature    1993..2364
                     /label=VP16
     CDS             1993..2364
                     /label=BBa_K1150001
     CDS             2373..2393
                     /label=NLS
     misc_feature    2373..2393
                     /label=BBa_K1150010
     misc_feature    2402..2623
                     /label=BGH
     terminator      2402..2623
                     /label=BBa_K1150012
ORIGIN
        1 ttcgttaccg ctactagatg gtttccggag tcgggggtag tggcggtggc cgtggcggtg
       61 gccgtggcgg agaagaagaa ccgtcgtcaa gtcacactcc taataaccga agaggaggag
      121 aacaagctca atcgtcggga acgaaatctc tcagaccaag aagcaacact gaatcaatga
      181 gcaaagcaat tcaacagtac accgtcgacg caagactcca cgccgttttc gaacaatccg
      241 gcgaatcagg gaaatcattc gactactcac aatcactcaa aacgacgacg tacggttcct
      301 ctgtacctga gcaacagatc acagcttatc tctctcgaat ccagcgaggt ggttacattc
      361 agcctttcgg atgtatgatc gccgtcgatg aatccagttt ccggatcatc ggttacagtg
      421 aaaacgccag agaaatgtta gggattatgc ctcaatctgt tcctactctt gagaaacctg
      481 agattctagc tatgggaact gatgtgagat ctttgttcac ttcttcgagc tcgattctac
      541 tcgagcgtgc tttcgttgct cgagagatta ccttgttaaa tccggtttgg atccattcca
      601 agaatactgg taaaccgttt tacgccattc ttcataggat tgatgttggt gttgttattg
      661 atttagagcc agctagaact gaagatcctg cgctttctat tgctggtgct gttcaatcgc
      721 agaaactcgc ggttcgtgcg atttctcagt tacaggctct tcctggtgga gatattaagc
      781 ttttgtgtga cactgtcgtg gaaagtgtga gggacttgac tggttatgat cgtgttatgg
      841 tttataagtt tcatgaagat gagcatggag aagttgtagc tgagagtaaa cgagatgatt
      901 tagagcctta tattggactg cattatcctg ctactgatat tcctcaagcg tcaaggttct
      961 tgtttaagca gaaccgtgtc cgaatgatag tagattgcaa tgccacacct gttcttgtgg
     1021 tccaggacga taggctaact cagtctatgt gcttggttgg ttctactctt agggctcctc
     1081 atggttgtca ctctcagtat atggctaaca tgggatctat tgcgtcttta gcaatggcgg
     1141 ttataatcaa tggaaatgaa gatgatggga gcaatgtagc tagtggaaga agctcgatga
     1201 ggctttgggg tttggttgtt tgccatcaca cttcttctcg ctgcataccg tttccgctaa
     1261 ggtatgcttg tgagtttttg atgcaggctt tcggtttaca gttaaacatg gaattgcagt
     1321 tagctttgca aatgtcagag aaacgcgttt tgagaacgca gacactgtta tgtgatatgc
     1381 ttctgcgtga ctcgcctgct ggaattgtta cacagagtcc cagtatcatg gacttagtga
     1441 aatgtgacgg tgcagcattt ctttaccacg ggaagtatta cccgttgggt gttgctccta
     1501 gtgaagttca gataaaagat gttgtggagt ggttgcttgc gaatcatgcg gattcaaccg
     1561 gattaagcac tgatagttta ggcgatgcgg ggtatcccgg tgcagctgcg ttaggggatg
     1621 ctgtgtgcgg tatggcagtt gcatatatca caaaaagaga ctttcttttt tggtttcgat
     1681 ctcacactgc gaaagaaatc aaatggggag gcgctaagca tcatccggag gataaagatg
     1741 atgggcaacg aatgcatcct cgttcgtcct ttcaggcttt tcttgaagtt gttaagagcc
     1801 ggagtcagcc atgggaaact gcggaaatgg atgcgattca ctcgctccag cttattctga
     1861 gagactcttt taaagaatct gaggcggcta tgaactctaa agttgtggat ggtgtggttc
     1921 agccatgtag ggatatggcg ggggaacagg ggattgatga gttaggttac tagaggcagg
     1981 ctcctactag aggcgcgtac gaaaaacaat tacgggtcta ccatcgaggg cctgctcgat
     2041 ctcccggacg acgacgcccc cgaagaggcg gggctggcgg ctccgcgcct gtcctttctc
     2101 cccgcgggac acacgcgcag actgtcgacg gcccccccga ccgatgtcag cctgggggac
     2161 gagctccact tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc
     2221 gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc
     2281 gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc
     2341 cttggaattg acgagtacgg tgggtactag agcctgcaag aaaaagattg aattactaga
     2401 ggtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     2461 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     2521 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     2581 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atg
//