BBa_K1471011 1 BBa_K1471011 RBS (Arabidopsis Thaliana). 2014-10-13T11:00:00Z 2015-05-08T01:10:39Z Arabidopsis thaliana genome sequence. The initiation of protein biosynthesis is a major determinant of the efficiency of gene expression at the translational level. It is known that the nucleotide sequences around the AUG translation initiation codon act as an important signal to trigger the initiation of the translation event. (Kozak, 1987) false false _1850_ 0 16464 9 Not in stock false We had to discuss what was the best option to enhance the expression efficiency of our proteins of interest to bio-remediate heavy metals, as mercury. false Juan Noe Hernandez Salazar annotation2419570 1 Receptor codon. range2419570 1 8 10 annotation2419434 1 Kozak Sequence. range2419434 1 1 11 annotation2419569 1 Ribosome binds to 5' end. range2419569 1 1 1 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K1471000 1 BBa_K1471000 MerE. 2014-10-07T11:00:00Z 2015-07-22T02:39:55Z Bacterial operon mer. For mercury bioaccumulation. false false _1850_ 4206 16464 9 It's complicated false We have to optimizes its codons in Arabidopsis Thaliana and removed the restriction sites for EcoR1, Xba1, Spe1 and Pst1. false Juan No?? Hern??ndez Salazar BBa_K1471001 1 BBa_K1471001 MerB. 2014-10-07T11:00:00Z 2015-07-22T02:40:08Z Genomic sequence The MerB gene codes for the proetin which function is to cleave the organic part of the molecule of methyl-mercury false false _1850_ 4206 16614 9 It's complicated false efwgeg false Jaime Antonio del Castillo Nu??ez annotation2419568 1 MerB range2419568 1 1 639 BBa_K1471012 1 BBa_K1471012 RBS - MerE - RBS - MerB - RBS - GFP 2014-11-02T12:00:00Z 2015-05-08T01:10:39Z Mer genes comes from bacterial genomic sequences This composed part has a green light reporter with two coding genes. false false _1850_ 0 16614 9 Not in stock false We tried the system in a prokaryote chassis, e. coli. false Jaime Antonio del Castillo Nu??ez component2430116 1 BBa_K1471000 component2430110 1 BBa_K1471011 component2430115 1 BBa_K1471011 component2430118 1 BBa_E0040 component2430112 1 BBa_K1471001 annotation2430110 1 BBa_K1471011 range2430110 1 1 11 annotation2430112 1 BBa_K1471001 range2430112 1 18 656 annotation2430118 1 BBa_E0040 range2430118 1 868 1587 annotation2430115 1 BBa_K1471011 range2430115 1 665 675 annotation2430116 1 BBa_K1471000 range2430116 1 682 861 BBa_K1471012_sequence 1 aagcaatggcgtactagatgaagttggctccttatattttagaactactgacatcagtcaatcgaactaatggtacagctgatctcttggttcctttgcttagagagctggcaaagggaagaccagtttcacgtacaactcttgctggtatattggattggccagcagagagagtcgccgctgtgcttgagcaagctacctctactgagtacgataaagacggtaatatcatcggttatggcttgacattgagagagacgtcgtacgtgttcgagatagatgatagaagactctacgcttggtgtgctttggatacgctaattttcccagcattgatcggaagaaccgcacgagtatctagtcattgtgctgctaccggtgctccagtgagcctcaccgtttcgcccagtgagattcaagctgtggagccggctgggatggcagtctccctggttctgcctcaggaggccgccgatgtccgtcaatcattctgttgccatgttcactttttcgctagtgtccctacagctgaggattgggctagtaaacaccaggggttggaagggttggctatagtgtcagtgcatgaagctttcggactaggacaggagttcaatcgtcatttattgcagacgatgagttccagaactccgtaatactagagaagcaatggcgtactagatgcgggccaagtccgctattttctcacgcacatcgcttagtctatgctctgcaagactactcgcgtccagtcagtgggttccgtcttcctccaggaatagctcggcgatctcgtcacgcttaaatcctagtaggtgcgcagactttacaaactttacccggacaacatcagcttctccctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1471000_sequence 1 atgcgggccaagtccgctattttctcacgcacatcgcttagtctatgctctgcaagactactcgcgtccagtcagtgggttccgtcttcctccaggaatagctcggcgatctcgtcacgcttaaatcctagtaggtgcgcagactttacaaactttacccggacaacatcagcttctccc BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1471001_sequence 1 atgaagttggctccttatattttagaactactgacatcagtcaatcgaactaatggtacagctgatctcttggttcctttgcttagagagctggcaaagggaagaccagtttcacgtacaactcttgctggtatattggattggccagcagagagagtcgccgctgtgcttgagcaagctacctctactgagtacgataaagacggtaatatcatcggttatggcttgacattgagagagacgtcgtacgtgttcgagatagatgatagaagactctacgcttggtgtgctttggatacgctaattttcccagcattgatcggaagaaccgcacgagtatctagtcattgtgctgctaccggtgctccagtgagcctcaccgtttcgcccagtgagattcaagctgtggagccggctgggatggcagtctccctggttctgcctcaggaggccgccgatgtccgtcaatcattctgttgccatgttcactttttcgctagtgtccctacagctgaggattgggctagtaaacaccaggggttggaagggttggctatagtgtcagtgcatgaagctttcggactaggacaggagttcaatcgtcatttattgcagacgatgagttccagaactccgtaa BBa_K1471011_sequence 1 aagcaatggcg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z