BBa_K1472001 1 BBa_K1472001 Testing 2014-09-15T11:00:00Z 2015-05-08T01:10:39Z The genome sequence of Escherichia coli K-12 substr. W3110 is available in GenBank at http://www.ncbi.nlm.nih.gov/gene/12930860. Escherichia coli thioesterase without a leader peptide sequence (???TesA) We have cloned a modified thioesterase gene, ???tesA, which codes for an E. coli thioesterase protein that lacks the N-terminal 26-amino acid signal peptide sequence, and is named here as ???leaderless TesA??? or ???TesA. The ???TesA protein is expected to be expressed exclusively in the cytosol of E. coli to catalyze the conversion of fatty acyl-ACP (acyl carrier protein) or fatty acyl-CoA to free fatty acids (Figure 1). ???TesA is predicted to enhance free fatty acid production in the genetically-modified E. coli strain. false false _1851_ 0 22977 9 No part sequence false N/A false igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z