BBa_K1472608 1 BBa_K1472608 Oleic acid (18:0) -> alpha-Linolenic acid (18:3-9,12,15) 2014-09-24T11:00:00Z 2015-05-08T01:10:39Z The three desaturase genes are synthesized according to Thrausochytrid with E. coli optimization. It is a device that contained a promoter (BBa_R0010), RBS (BBa_B0034) for each desaturase gene ∆9,12 and ∆15). When free fatty acids, for example, stearic acid (18:0) enters cell, it is converted into oleic acid (18:1∆9) by ∆9 desaturase, then to linolenic acid (18:2∆9,12) by ∆12 desaturase and finally to alpha-Linolenic acid, ALA (18:3∆9,12,15) by ∆15 desaturase. This device is designated to express under the presence of IPTG. false false _1851_ 0 22971 9 No part sequence false The length of the whole device is considered relatively large, it increases the difficulty during cloning procedures. false Lau Tsz Kit annotation2386403 1 conserved range2386403 1 213 216 annotation2386400 1 -10 range2386400 1 161 166 annotation2386401 1 LacI binding site range2386401 1 166 200 annotation2386399 1 -35 range2386399 1 137 142 annotation2386397 1 end of LacI coding region (inactive) range2386397 1 1 88 annotation2386404 1 BBa_B0034 range2386404 1 209 220 annotation2386398 1 CAP binding site range2386398 1 89 126 annotation2386402 1 start range2386402 1 173 173 annotation2386396 1 BBa_R0010 range2386396 1 1 200 igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z