LOCUS       BBa_K1472611            3384 bp    DNA     linear       21-May-2024
DEFINITION  IPTG inducible expression of ∆9, ∆12, and ∆15 
            desaturases
ACCESSION   BBa_K1472611
VERSION     BBa_K1472611.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start
     misc_feature    1181..1186
                     /label=Stop
     CDS             227..1186
                     /label=BBa_K1027001
     misc_feature    1199..1202
                     /label=conserved
     RBS             1195..1206
                     /label=BBa_B0034
     misc_feature    1213..1215
                     /label=Start
     misc_feature    2269..2274
                     /label=Stop
     CDS             1213..2274
                     /label=BBa_K1027002
     misc_feature    2287..2290
                     /label=conserved
     RBS             2283..2294
                     /label=BBa_B0034
     CDS             2301..3404
                     /label=Delta 15 desaturase
     CDS             2301..3384
                     /label=BBa_K1472610
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc tgaatccgct
      241 gaatattgaa tatctgtacc tgagcaaact gttcgataat agcctgatcg tgtttaacaa
      301 acgtcagctg tttcgttttt ttgtgcgctt ttttttcatg accgcagcac tgccgaatga
      361 tagcaaaccg aaactgacac cggcatggac cgtgattttt ttctttacca gcattcatct
      421 ggttgcactg ctggcatttc tgccgcagtt ttttagctgg aaagcagttg gtatggcctt
      481 tctgctgtat gttattaccg gtggtattgg tattaccctg ggttttcatc gttgtattag
      541 ccatcgtagc tttaatgttc cgaaatggct ggaatatatc tttgttattt gtggcaccct
      601 ggcctgtcag ggtggtgttt ttgaatgggt tggtctgcat cgtatgcatc acaaatttag
      661 cgataccaca ccggatccgc atgatagcaa taaaggtttt tggtggtcac atattggctg
      721 gatgatgttt gaaattccgg caaaagcaga tattccgcgt tataccaaag atatccagga
      781 cgataaattc taccagttct gccagaataa cctgattctg attcaggttg cactgggtct
      841 gattctgttt gccctgggtg gttggccgtt tgttatttgg ggtatttttg ttcgtctggt
      901 gtttgtgttt cactttacct ggtttgttaa tagcgccacc cataaatttg gttatgttag
      961 ccatgagagc aacgattata gccgtaattg ttggtgggtt gccctgctga cctttggtga
     1021 aggttggcat aataatcatc acgcctatca gtatagcgca cgtcatggtc tgcaatggtg
     1081 ggaagttgat ctgacctgga tgaccatcaa atttctgagc ctgctgggtc tggcaaaaga
     1141 tattaaactg cctccggaaa ccgcaatggc aaataaagcc taataatact agagaaagag
     1201 gagaaatact agatgaccgc aaccattccg cctctgaccc cgaccgttac cccgagcaat
     1261 ccggatcgtc cgattgcaga tctgaaactg caagatatta tcaaaaccct gccgaaagaa
     1321 tgcttcgaaa aaaaagcaag caaagcatgg gcaagcgttc tgattaccct gggtgcaatt
     1381 gcagttggtt atctgggtat tatctatctg ccgtggtatt gtctgccgat tacctggatt
     1441 tggaccggca ccgcactgac cggtgcattt gttgttggtc atgattgtgg tcatcgtagc
     1501 tttgcaaaaa aacgttgggt taatgatctg gtgggtcata ttgcatttgc accgctgatt
     1561 tatccgtttc atagctggcg tctgctgcat gatcatcatc atctgcatac caacaaaatc
     1621 gaagttgata atgcatggga tccgtggtca gttgaagcat ttcaggcaag tccggcaatt
     1681 gttcgtctgt tttatcgtgc aattcgtggt ccgttttggt ggaccggtag catttttcat
     1741 tggagcctga tgcattttaa actgagcaat tttgcacagc gcgatcgcaa taaagtgaaa
     1801 ctgagcattg cagtggtttt tctgtttgca gcaattgcat ttccggcact gattattacc
     1861 accggtgttt ggggttttgt gaaattttgg ctgatgccgt ggctggttta tcatttttgg
     1921 atgagcacct ttaccatcgt gcatcatacc attccggaaa ttcgttttcg tccggcagca
     1981 gattggagcg cagccgaagc acagctgaat ggcaccgttc attgtgatta tccgcgttgg
     2041 gttgaagttc tgtgccatga tattaatgtg catattccgc atcatctgag cgttgcaatt
     2101 ccgagctata atctgcgtct ggcacatggt agcctgaaag aaaattgggg tccgtttctg
     2161 tatgaacgca cctttaattg gcagctgatg cagcagatta gcggtcagtg tcatctgtat
     2221 gatccggaac atggttatcg tacctttggc agcctgaaaa aagtgtaata atgatactag
     2281 agaaagagga gaaatactag atgcgtctgg aaattagcag tccgcagacc aaactgccgt
     2341 atccgaaaac cgaagaactg ccgtttaccc tgcaagaact gcgtaatgca attccggcag
     2401 attgttttga accgagcgtt gttcgtagcc tgggttattt ctttctggat gttggtctga
     2461 ttgcaggttt ttatgcactg gcagcatatc tggatagctg gtttttctat ccgatttttt
     2521 ggctgattca gggcaccctg ttttggagcc tgtttgttgt tggtcatgat tgtggtcatg
     2581 gtagctttag caaaagcaaa accctgaata attggattgg tcatctgagc cataccccga
     2641 ttctggttcc gtatcatggt tggcgtatta gccatcgtac ccatcatgca aataccggta
     2701 atattgatac cgatgaaagc tggtatccgg ttagcgaaca gaaatataac cagatggcat
     2761 ggtatgaaaa actgctgcgt ttttatctgc cgctgattgc atatccgatt tacctgtttc
     2821 gtcgtagccc gaatcgtcag ggtagccatt ttatgcctgg tagtccgctg tttcgtccgg
     2881 gtgaaaaagc agcagttctg accagcacct ttgccctggc agcatttgtt ggttttctgg
     2941 gctttctgac ctggcagttt ggttggctgt ttctgctgaa attctatgtt gcaccgtatc
     3001 tggtttttgt tgtttggctg gatctggtta cctttctgca tcataccgaa gataacattc
     3061 cgtggtatcg tggtgatgat tggtattttc tgaaaggtgc actgagcacc attgatcgtg
     3121 attatggttt tatcaacccg atccatcatg atattggcac ccatgttgca catcatatct
     3181 ttagcaatat gccgcactat aaactgcgtc gtgcaaccga agcaattaaa ccgattctgg
     3241 gtgaatatta tcgttatagc gacgaaccga tttggcaggc atttttcaaa agctattggg
     3301 cctgtcattt tgttccgaat cagggtagcg gtgtttatta tcagagcccg agcaatggtg
     3361 gttatcagaa aaaaccgtaa taat
//