BBa_K1027001 1 BBa_K1027001 Delta 9 Desaturase, optimised for expression in Escherichia coli 2013-09-18T11:00:00Z 2015-05-08T01:08:46Z Synthesized gene based off the protein sequence from Synechocystis sp This enzyme is obtained fro the gene Stearoly Co-A Desaturase (SCD). The gene is obtained from Synechocystis sp., a cyanobacterium.It is involved in the biosynthesize of C18:1 from C18:0. C18:1 corresponds to Oleic acid. The delta 9 desaturase introduces cis carbon double bond on the 9th carbon in C18:0. false false _1334_ 0 15689 9 In stock true Codon optimized for expression in E. coli false Robert Harrison, Lorna Hepworth, Vun Hiang Tan annotation2357079 1 Stop range2357079 1 955 960 annotation2357078 1 Start range2357078 1 1 3 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1027002 1 BBa_K1027002 Delta 12 Desaturase, optimised for expression in Escherichia coli 2013-09-18T11:00:00Z 2015-05-08T01:08:46Z Produced synthetically based on protein sequence from Synechocystis sp. This enzyme is obtained from the gene Oleic acid desaturase (ode).The gene is obtained from Synechocystis sp., a cyanobacterium and introduced in the E.coli. It is a trans-membrane enzyme, involved in the biosynthesis of C18:2 from C18:1. C18:2 corresponds to Linolenic acid. The delta 12 desaturase introduces a second cis carbon double bond on the 12th carbon in C18:1. false false _1334_ 0 15689 9 In stock true Codon optimization for expression in E coli false Robert Harrison, Lorna Hepworth, Vun Hiang Tan annotation2354834 1 Start range2354834 1 1 3 annotation2354835 1 Stop range2354835 1 1057 1062 BBa_K1472612 1 BBa_K1472612 Delta 9,12 and 15 Desaturase 2014-09-28T11:00:00Z 2015-05-08T01:10:39Z All the desaturase sequences are derived from the Synechocystis sp. but optimised for the expression in E. coli. This translational unit is composed of delta9, delta 12 and delta 15 desaturase genes with RBS for each of them. Desaturase introduces a cis-carbon double bond to fatty acid carbon chain. &#8710; 9 desaturase introduce double bond to stearic acid at the carbon 9 position to produce oleic acid. &#8710;12 desaturase introduce double bond at carbon 12 of oleic acid so to produce linolenic acid. At the end, &#8710;15 desaturase convert linolenic acid into alpha-linolenic acid by adding a double bond at carbon 15. false false _1851_ 0 23007 9 Not in stock false The desaturase genes in plasmids were introduced into E. coli for amplification. The final construct includes iGEM provided RBS (BBa_B0034). false Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu component2389022 1 BBa_B0034 component2389025 1 BBa_K1027002 component2389020 1 BBa_K1027001 component2389027 1 BBa_B0034 component2389017 1 BBa_B0034 component2389028 1 BBa_K1472610 annotation2389022 1 BBa_B0034 range2389022 1 987 998 annotation2389017 1 BBa_B0034 range2389017 1 1 12 annotation2389027 1 BBa_B0034 range2389027 1 2075 2086 annotation2389028 1 BBa_K1472610 range2389028 1 2095 3218 annotation2389020 1 BBa_K1027001 range2389020 1 19 978 annotation2389025 1 BBa_K1027002 range2389025 1 1005 2066 BBa_K1472610 1 BBa_K1472610 Delta 15 desaturase 2014-09-27T11:00:00Z 2015-05-08T01:10:39Z The amino acid sequence of delta 15 desaturase is derived from Synechocystis sp. but codon optimised for the expression in E. coli. This sequence encoded for the delta15 desaturase. The gene is from Synechocystis sp. and optimised for the expression in E. coli. During the fatty acid conversion process, delta 15 desaturase catalyses the conversion of linolenic acid (18:2) into alpha-Linolenic acid, ALA (18:3) by adding a cis-doube bond at carbon 15. false false _1851_ 0 23007 9 In stock false The enzyme cut site must be designed according to iGEM prefix and suffix requirements. false Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu annotation2390390 1 Delta 15 desaturase range2390390 1 1 1104 BBa_K1472610_sequence 1 atgcgtctggaaattagcagtccgcagaccaaactgccgtatccgaaaaccgaagaactgccgtttaccctgcaagaactgcgtaatgcaattccggcagattgttttgaaccgagcgttgttcgtagcctgggttatttctttctggatgttggtctgattgcaggtttttatgcactggcagcatatctggatagctggtttttctatccgattttttggctgattcagggcaccctgttttggagcctgtttgttgttggtcatgattgtggtcatggtagctttagcaaaagcaaaaccctgaataattggattggtcatctgagccataccccgattctggttccgtatcatggttggcgtattagccatcgtacccatcatgcaaataccggtaatattgataccgatgaaagctggtatccggttagcgaacagaaatataaccagatggcatggtatgaaaaactgctgcgtttttatctgccgctgattgcatatccgatttacctgtttcgtcgtagcccgaatcgtcagggtagccattttatgcctggtagtccgctgtttcgtccgggtgaaaaagcagcagttctgaccagcacctttgccctggcagcatttgttggttttctgggctttctgacctggcagtttggttggctgtttctgctgaaattctatgttgcaccgtatctggtttttgttgtttggctggatctggttacctttctgcatcataccgaagataacattccgtggtatcgtggtgatgattggtattttctgaaaggtgcactgagcaccattgatcgtgattatggttttatcaacccgatccatcatgatattggcacccatgttgcacatcatatctttagcaatatgccgcactataaactgcgtcgtgcaaccgaagcaattaaaccgattctgggtgaatattatcgttatagcgacgaaccgatttggcaggcatttttcaaaagctattgggcctgtcattttgttccgaatcagggtagcggtgtttattatcagagcccgagcaatggtggttatcagaaaaaaccgtaataat BBa_K1027001_sequence 1 atgctgaatccgctgaatattgaatatctgtacctgagcaaactgttcgataatagcctgatcgtgtttaacaaacgtcagctgtttcgtttttttgtgcgcttttttttcatgaccgcagcactgccgaatgatagcaaaccgaaactgacaccggcatggaccgtgatttttttctttaccagcattcatctggttgcactgctggcatttctgccgcagttttttagctggaaagcagttggtatggcctttctgctgtatgttattaccggtggtattggtattaccctgggttttcatcgttgtattagccatcgtagctttaatgttccgaaatggctggaatatatctttgttatttgtggcaccctggcctgtcagggtggtgtttttgaatgggttggtctgcatcgtatgcatcacaaatttagcgataccacaccggatccgcatgatagcaataaaggtttttggtggtcacatattggctggatgatgtttgaaattccggcaaaagcagatattccgcgttataccaaagatatccaggacgataaattctaccagttctgccagaataacctgattctgattcaggttgcactgggtctgattctgtttgccctgggtggttggccgtttgttatttggggtatttttgttcgtctggtgtttgtgtttcactttacctggtttgttaatagcgccacccataaatttggttatgttagccatgagagcaacgattatagccgtaattgttggtgggttgccctgctgacctttggtgaaggttggcataataatcatcacgcctatcagtatagcgcacgtcatggtctgcaatggtgggaagttgatctgacctggatgaccatcaaatttctgagcctgctgggtctggcaaaagatattaaactgcctccggaaaccgcaatggcaaataaagcctaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1472612_sequence 1 aaagaggagaaatactagatgctgaatccgctgaatattgaatatctgtacctgagcaaactgttcgataatagcctgatcgtgtttaacaaacgtcagctgtttcgtttttttgtgcgcttttttttcatgaccgcagcactgccgaatgatagcaaaccgaaactgacaccggcatggaccgtgatttttttctttaccagcattcatctggttgcactgctggcatttctgccgcagttttttagctggaaagcagttggtatggcctttctgctgtatgttattaccggtggtattggtattaccctgggttttcatcgttgtattagccatcgtagctttaatgttccgaaatggctggaatatatctttgttatttgtggcaccctggcctgtcagggtggtgtttttgaatgggttggtctgcatcgtatgcatcacaaatttagcgataccacaccggatccgcatgatagcaataaaggtttttggtggtcacatattggctggatgatgtttgaaattccggcaaaagcagatattccgcgttataccaaagatatccaggacgataaattctaccagttctgccagaataacctgattctgattcaggttgcactgggtctgattctgtttgccctgggtggttggccgtttgttatttggggtatttttgttcgtctggtgtttgtgtttcactttacctggtttgttaatagcgccacccataaatttggttatgttagccatgagagcaacgattatagccgtaattgttggtgggttgccctgctgacctttggtgaaggttggcataataatcatcacgcctatcagtatagcgcacgtcatggtctgcaatggtgggaagttgatctgacctggatgaccatcaaatttctgagcctgctgggtctggcaaaagatattaaactgcctccggaaaccgcaatggcaaataaagcctaataatactagagaaagaggagaaatactagatgaccgcaaccattccgcctctgaccccgaccgttaccccgagcaatccggatcgtccgattgcagatctgaaactgcaagatattatcaaaaccctgccgaaagaatgcttcgaaaaaaaagcaagcaaagcatgggcaagcgttctgattaccctgggtgcaattgcagttggttatctgggtattatctatctgccgtggtattgtctgccgattacctggatttggaccggcaccgcactgaccggtgcatttgttgttggtcatgattgtggtcatcgtagctttgcaaaaaaacgttgggttaatgatctggtgggtcatattgcatttgcaccgctgatttatccgtttcatagctggcgtctgctgcatgatcatcatcatctgcataccaacaaaatcgaagttgataatgcatgggatccgtggtcagttgaagcatttcaggcaagtccggcaattgttcgtctgttttatcgtgcaattcgtggtccgttttggtggaccggtagcatttttcattggagcctgatgcattttaaactgagcaattttgcacagcgcgatcgcaataaagtgaaactgagcattgcagtggtttttctgtttgcagcaattgcatttccggcactgattattaccaccggtgtttggggttttgtgaaattttggctgatgccgtggctggtttatcatttttggatgagcacctttaccatcgtgcatcataccattccggaaattcgttttcgtccggcagcagattggagcgcagccgaagcacagctgaatggcaccgttcattgtgattatccgcgttgggttgaagttctgtgccatgatattaatgtgcatattccgcatcatctgagcgttgcaattccgagctataatctgcgtctggcacatggtagcctgaaagaaaattggggtccgtttctgtatgaacgcacctttaattggcagctgatgcagcagattagcggtcagtgtcatctgtatgatccggaacatggttatcgtacctttggcagcctgaaaaaagtgtaataatgatactagagaaagaggagaaatactagaggaattcgcggccgcttctagatgcgtctggaaattagcagtccgcagaccaaactgccgtatccgaaaaccgaagaactgccgtttaccctgcaagaactgcgtaatgcaattccggcagattgttttgaaccgagcgttgttcgtagcctgggttatttctttctggatgttggtctgattgcaggtttttatgcactggcagcatatctggatagctggtttttctatccgattttttggctgattcagggcaccctgttttggagcctgtttgttgttggtcatgattgtggtcatggtagctttagcaaaagcaaaaccctgaataattggattggtcatctgagccataccccgattctggttccgtatcatggttggcgtattagccatcgtacccatcatgcaaataccggtaatattgataccgatgaaagctggtatccggttagcgaacagaaatataaccagatggcatggtatgaaaaactgctgcgtttttatctgccgctgattgcatatccgatttacctgtttcgtcgtagcccgaatcgtcagggtagccattttatgcctggtagtccgctgtttcgtccgggtgaaaaagcagcagttctgaccagcacctttgccctggcagcatttgttggttttctgggctttctgacctggcagtttggttggctgtttctgctgaaattctatgttgcaccgtatctggtttttgttgtttggctggatctggttacctttctgcatcataccgaagataacattccgtggtatcgtggtgatgattggtattttctgaaaggtgcactgagcaccattgatcgtgattatggttttatcaacccgatccatcatgatattggcacccatgttgcacatcatatctttagcaatatgccgcactataaactgcgtcgtgcaaccgaagcaattaaaccgattctgggtgaatattatcgttatagcgacgaaccgatttggcaggcatttttcaaaagctattgggcctgtcattttgttccgaatcagggtagcggtgtttattatcagagcccgagcaatggtggttatcagaaaaaaccgtaataatactagtagcggccgctgcag BBa_K1027002_sequence 1 atgaccgcaaccattccgcctctgaccccgaccgttaccccgagcaatccggatcgtccgattgcagatctgaaactgcaagatattatcaaaaccctgccgaaagaatgcttcgaaaaaaaagcaagcaaagcatgggcaagcgttctgattaccctgggtgcaattgcagttggttatctgggtattatctatctgccgtggtattgtctgccgattacctggatttggaccggcaccgcactgaccggtgcatttgttgttggtcatgattgtggtcatcgtagctttgcaaaaaaacgttgggttaatgatctggtgggtcatattgcatttgcaccgctgatttatccgtttcatagctggcgtctgctgcatgatcatcatcatctgcataccaacaaaatcgaagttgataatgcatgggatccgtggtcagttgaagcatttcaggcaagtccggcaattgttcgtctgttttatcgtgcaattcgtggtccgttttggtggaccggtagcatttttcattggagcctgatgcattttaaactgagcaattttgcacagcgcgatcgcaataaagtgaaactgagcattgcagtggtttttctgtttgcagcaattgcatttccggcactgattattaccaccggtgtttggggttttgtgaaattttggctgatgccgtggctggtttatcatttttggatgagcacctttaccatcgtgcatcataccattccggaaattcgttttcgtccggcagcagattggagcgcagccgaagcacagctgaatggcaccgttcattgtgattatccgcgttgggttgaagttctgtgccatgatattaatgtgcatattccgcatcatctgagcgttgcaattccgagctataatctgcgtctggcacatggtagcctgaaagaaaattggggtccgtttctgtatgaacgcacctttaattggcagctgatgcagcagattagcggtcagtgtcatctgtatgatccggaacatggttatcgtacctttggcagcctgaaaaaagtgtaataatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z