BBa_K1472612
1
BBa_K1472612
Delta 9,12 and 15 Desaturase
2014-09-28T11:00:00Z
2015-05-08T01:10:39Z
All the desaturase sequences are derived from the Synechocystis sp. but optimised for the expression in E. coli.
This translational unit is composed of delta9, delta 12 and delta 15 desaturase genes with RBS for each of them. Desaturase introduces a cis-carbon double bond to fatty acid carbon chain. ∆ 9 desaturase introduce double bond to stearic acid at the carbon 9 position to produce oleic acid. ∆12 desaturase introduce double bond at carbon 12 of oleic acid so to produce linolenic acid. At the end, ∆15 desaturase convert linolenic acid into alpha-linolenic acid by adding a double bond at carbon 15.
false
false
_1851_
0
23007
9
Not in stock
false
The desaturase genes in plasmids were introduced into E. coli for amplification. The final construct includes iGEM provided RBS (BBa_B0034).
false
Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu
component2389025
1
BBa_K1027002
component2389028
1
BBa_K1472610
component2389020
1
BBa_K1027001
component2389017
1
BBa_B0034
component2389027
1
BBa_B0034
component2389022
1
BBa_B0034
annotation2389020
1
BBa_K1027001
range2389020
1
19
978
annotation2389025
1
BBa_K1027002
range2389025
1
1005
2066
annotation2389027
1
BBa_B0034
range2389027
1
2075
2086
annotation2389022
1
BBa_B0034
range2389022
1
987
998
annotation2389017
1
BBa_B0034
range2389017
1
1
12
annotation2389028
1
BBa_K1472610
range2389028
1
2095
3218
BBa_K1472610
1
BBa_K1472610
Delta 15 desaturase
2014-09-27T11:00:00Z
2015-05-08T01:10:39Z
The amino acid sequence of delta 15 desaturase is derived from Synechocystis sp. but codon optimised for the expression in E. coli.
This sequence encoded for the delta15 desaturase. The gene is from Synechocystis sp. and optimised for the expression in E. coli. During the fatty acid conversion process, delta 15 desaturase catalyses the conversion of linolenic acid (18:2) into alpha-Linolenic acid, ALA (18:3) by adding a cis-doube bond at carbon 15.
false
false
_1851_
0
23007
9
In stock
false
The enzyme cut site must be designed according to iGEM prefix and suffix requirements.
false
Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu
annotation2390390
1
Delta 15 desaturase
range2390390
1
1
1104
BBa_K1027001
1
BBa_K1027001
Delta 9 Desaturase, optimised for expression in Escherichia coli
2013-09-18T11:00:00Z
2015-05-08T01:08:46Z
Synthesized gene based off the protein sequence from Synechocystis sp
This enzyme is obtained fro the gene Stearoly Co-A Desaturase (SCD). The gene is obtained from Synechocystis sp., a cyanobacterium.It is involved in the biosynthesize of C18:1 from C18:0. C18:1 corresponds to Oleic acid. The delta 9 desaturase introduces cis carbon double bond on the 9th carbon in C18:0.
false
false
_1334_
0
15689
9
In stock
true
Codon optimized for expression in E. coli
false
Robert Harrison, Lorna Hepworth, Vun Hiang Tan
annotation2357078
1
Start
range2357078
1
1
3
annotation2357079
1
Stop
range2357079
1
955
960
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1027002
1
BBa_K1027002
Delta 12 Desaturase, optimised for expression in Escherichia coli
2013-09-18T11:00:00Z
2015-05-08T01:08:46Z
Produced synthetically based on protein sequence from Synechocystis sp.
This enzyme is obtained from the gene Oleic acid desaturase (ode).The gene is obtained from Synechocystis sp., a cyanobacterium and introduced in the E.coli. It is a trans-membrane enzyme, involved in the biosynthesis of C18:2 from C18:1. C18:2 corresponds to Linolenic acid. The delta 12 desaturase introduces a second cis carbon double bond on the 12th carbon in C18:1.
false
false
_1334_
0
15689
9
In stock
true
Codon optimization for expression in E coli
false
Robert Harrison, Lorna Hepworth, Vun Hiang Tan
annotation2354834
1
Start
range2354834
1
1
3
annotation2354835
1
Stop
range2354835
1
1057
1062
BBa_K1472610_sequence
1
atgcgtctggaaattagcagtccgcagaccaaactgccgtatccgaaaaccgaagaactgccgtttaccctgcaagaactgcgtaatgcaattccggcagattgttttgaaccgagcgttgttcgtagcctgggttatttctttctggatgttggtctgattgcaggtttttatgcactggcagcatatctggatagctggtttttctatccgattttttggctgattcagggcaccctgttttggagcctgtttgttgttggtcatgattgtggtcatggtagctttagcaaaagcaaaaccctgaataattggattggtcatctgagccataccccgattctggttccgtatcatggttggcgtattagccatcgtacccatcatgcaaataccggtaatattgataccgatgaaagctggtatccggttagcgaacagaaatataaccagatggcatggtatgaaaaactgctgcgtttttatctgccgctgattgcatatccgatttacctgtttcgtcgtagcccgaatcgtcagggtagccattttatgcctggtagtccgctgtttcgtccgggtgaaaaagcagcagttctgaccagcacctttgccctggcagcatttgttggttttctgggctttctgacctggcagtttggttggctgtttctgctgaaattctatgttgcaccgtatctggtttttgttgtttggctggatctggttacctttctgcatcataccgaagataacattccgtggtatcgtggtgatgattggtattttctgaaaggtgcactgagcaccattgatcgtgattatggttttatcaacccgatccatcatgatattggcacccatgttgcacatcatatctttagcaatatgccgcactataaactgcgtcgtgcaaccgaagcaattaaaccgattctgggtgaatattatcgttatagcgacgaaccgatttggcaggcatttttcaaaagctattgggcctgtcattttgttccgaatcagggtagcggtgtttattatcagagcccgagcaatggtggttatcagaaaaaaccgtaataat
BBa_K1027001_sequence
1
atgctgaatccgctgaatattgaatatctgtacctgagcaaactgttcgataatagcctgatcgtgtttaacaaacgtcagctgtttcgtttttttgtgcgcttttttttcatgaccgcagcactgccgaatgatagcaaaccgaaactgacaccggcatggaccgtgatttttttctttaccagcattcatctggttgcactgctggcatttctgccgcagttttttagctggaaagcagttggtatggcctttctgctgtatgttattaccggtggtattggtattaccctgggttttcatcgttgtattagccatcgtagctttaatgttccgaaatggctggaatatatctttgttatttgtggcaccctggcctgtcagggtggtgtttttgaatgggttggtctgcatcgtatgcatcacaaatttagcgataccacaccggatccgcatgatagcaataaaggtttttggtggtcacatattggctggatgatgtttgaaattccggcaaaagcagatattccgcgttataccaaagatatccaggacgataaattctaccagttctgccagaataacctgattctgattcaggttgcactgggtctgattctgtttgccctgggtggttggccgtttgttatttggggtatttttgttcgtctggtgtttgtgtttcactttacctggtttgttaatagcgccacccataaatttggttatgttagccatgagagcaacgattatagccgtaattgttggtgggttgccctgctgacctttggtgaaggttggcataataatcatcacgcctatcagtatagcgcacgtcatggtctgcaatggtgggaagttgatctgacctggatgaccatcaaatttctgagcctgctgggtctggcaaaagatattaaactgcctccggaaaccgcaatggcaaataaagcctaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1472612_sequence
1
aaagaggagaaatactagatgctgaatccgctgaatattgaatatctgtacctgagcaaactgttcgataatagcctgatcgtgtttaacaaacgtcagctgtttcgtttttttgtgcgcttttttttcatgaccgcagcactgccgaatgatagcaaaccgaaactgacaccggcatggaccgtgatttttttctttaccagcattcatctggttgcactgctggcatttctgccgcagttttttagctggaaagcagttggtatggcctttctgctgtatgttattaccggtggtattggtattaccctgggttttcatcgttgtattagccatcgtagctttaatgttccgaaatggctggaatatatctttgttatttgtggcaccctggcctgtcagggtggtgtttttgaatgggttggtctgcatcgtatgcatcacaaatttagcgataccacaccggatccgcatgatagcaataaaggtttttggtggtcacatattggctggatgatgtttgaaattccggcaaaagcagatattccgcgttataccaaagatatccaggacgataaattctaccagttctgccagaataacctgattctgattcaggttgcactgggtctgattctgtttgccctgggtggttggccgtttgttatttggggtatttttgttcgtctggtgtttgtgtttcactttacctggtttgttaatagcgccacccataaatttggttatgttagccatgagagcaacgattatagccgtaattgttggtgggttgccctgctgacctttggtgaaggttggcataataatcatcacgcctatcagtatagcgcacgtcatggtctgcaatggtgggaagttgatctgacctggatgaccatcaaatttctgagcctgctgggtctggcaaaagatattaaactgcctccggaaaccgcaatggcaaataaagcctaataatactagagaaagaggagaaatactagatgaccgcaaccattccgcctctgaccccgaccgttaccccgagcaatccggatcgtccgattgcagatctgaaactgcaagatattatcaaaaccctgccgaaagaatgcttcgaaaaaaaagcaagcaaagcatgggcaagcgttctgattaccctgggtgcaattgcagttggttatctgggtattatctatctgccgtggtattgtctgccgattacctggatttggaccggcaccgcactgaccggtgcatttgttgttggtcatgattgtggtcatcgtagctttgcaaaaaaacgttgggttaatgatctggtgggtcatattgcatttgcaccgctgatttatccgtttcatagctggcgtctgctgcatgatcatcatcatctgcataccaacaaaatcgaagttgataatgcatgggatccgtggtcagttgaagcatttcaggcaagtccggcaattgttcgtctgttttatcgtgcaattcgtggtccgttttggtggaccggtagcatttttcattggagcctgatgcattttaaactgagcaattttgcacagcgcgatcgcaataaagtgaaactgagcattgcagtggtttttctgtttgcagcaattgcatttccggcactgattattaccaccggtgtttggggttttgtgaaattttggctgatgccgtggctggtttatcatttttggatgagcacctttaccatcgtgcatcataccattccggaaattcgttttcgtccggcagcagattggagcgcagccgaagcacagctgaatggcaccgttcattgtgattatccgcgttgggttgaagttctgtgccatgatattaatgtgcatattccgcatcatctgagcgttgcaattccgagctataatctgcgtctggcacatggtagcctgaaagaaaattggggtccgtttctgtatgaacgcacctttaattggcagctgatgcagcagattagcggtcagtgtcatctgtatgatccggaacatggttatcgtacctttggcagcctgaaaaaagtgtaataatgatactagagaaagaggagaaatactagaggaattcgcggccgcttctagatgcgtctggaaattagcagtccgcagaccaaactgccgtatccgaaaaccgaagaactgccgtttaccctgcaagaactgcgtaatgcaattccggcagattgttttgaaccgagcgttgttcgtagcctgggttatttctttctggatgttggtctgattgcaggtttttatgcactggcagcatatctggatagctggtttttctatccgattttttggctgattcagggcaccctgttttggagcctgtttgttgttggtcatgattgtggtcatggtagctttagcaaaagcaaaaccctgaataattggattggtcatctgagccataccccgattctggttccgtatcatggttggcgtattagccatcgtacccatcatgcaaataccggtaatattgataccgatgaaagctggtatccggttagcgaacagaaatataaccagatggcatggtatgaaaaactgctgcgtttttatctgccgctgattgcatatccgatttacctgtttcgtcgtagcccgaatcgtcagggtagccattttatgcctggtagtccgctgtttcgtccgggtgaaaaagcagcagttctgaccagcacctttgccctggcagcatttgttggttttctgggctttctgacctggcagtttggttggctgtttctgctgaaattctatgttgcaccgtatctggtttttgttgtttggctggatctggttacctttctgcatcataccgaagataacattccgtggtatcgtggtgatgattggtattttctgaaaggtgcactgagcaccattgatcgtgattatggttttatcaacccgatccatcatgatattggcacccatgttgcacatcatatctttagcaatatgccgcactataaactgcgtcgtgcaaccgaagcaattaaaccgattctgggtgaatattatcgttatagcgacgaaccgatttggcaggcatttttcaaaagctattgggcctgtcattttgttccgaatcagggtagcggtgtttattatcagagcccgagcaatggtggttatcagaaaaaaccgtaataatactagtagcggccgctgcag
BBa_K1027002_sequence
1
atgaccgcaaccattccgcctctgaccccgaccgttaccccgagcaatccggatcgtccgattgcagatctgaaactgcaagatattatcaaaaccctgccgaaagaatgcttcgaaaaaaaagcaagcaaagcatgggcaagcgttctgattaccctgggtgcaattgcagttggttatctgggtattatctatctgccgtggtattgtctgccgattacctggatttggaccggcaccgcactgaccggtgcatttgttgttggtcatgattgtggtcatcgtagctttgcaaaaaaacgttgggttaatgatctggtgggtcatattgcatttgcaccgctgatttatccgtttcatagctggcgtctgctgcatgatcatcatcatctgcataccaacaaaatcgaagttgataatgcatgggatccgtggtcagttgaagcatttcaggcaagtccggcaattgttcgtctgttttatcgtgcaattcgtggtccgttttggtggaccggtagcatttttcattggagcctgatgcattttaaactgagcaattttgcacagcgcgatcgcaataaagtgaaactgagcattgcagtggtttttctgtttgcagcaattgcatttccggcactgattattaccaccggtgtttggggttttgtgaaattttggctgatgccgtggctggtttatcatttttggatgagcacctttaccatcgtgcatcataccattccggaaattcgttttcgtccggcagcagattggagcgcagccgaagcacagctgaatggcaccgttcattgtgattatccgcgttgggttgaagttctgtgccatgatattaatgtgcatattccgcatcatctgagcgttgcaattccgagctataatctgcgtctggcacatggtagcctgaaagaaaattggggtccgtttctgtatgaacgcacctttaattggcagctgatgcagcagattagcggtcagtgtcatctgtatgatccggaacatggttatcgtacctttggcagcctgaaaaaagtgtaataatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z